Align DUF5009 domain-containing protein (characterized, see rationale)
to candidate 202605 SO3504 conserved hypothetical protein (NCBI ptt file)
Query= uniprot:A0KZW6 (395 letters) >FitnessBrowser__MR1:202605 Length = 395 Score = 733 bits (1891), Expect = 0.0 Identities = 356/395 (90%), Positives = 368/395 (93%) Query: 1 MSTTAPESITNTGVNAQEAAAKKRQSKPRLMSLDALRGFDMFWILGGEALFGALLMLTGW 60 MSTTAPE N +NAQ A A Q KPRLMSLDALRGFDMFWILGGEALFGALL+ TGW Sbjct: 1 MSTTAPELAANVSINAQVATANNSQPKPRLMSLDALRGFDMFWILGGEALFGALLIFTGW 60 Query: 61 AGWQWGDTQMHHSEWNGFRFYDLIFPLFIFLSGVALGLSPKRLDKLPMQERMPVYRHGIK 120 AGWQWGDTQMHHSEW+GFR YDLIFPLFIFLSGVALGLSPKRLDKLP+ ER+PVYRHG+K Sbjct: 61 AGWQWGDTQMHHSEWHGFRLYDLIFPLFIFLSGVALGLSPKRLDKLPLHERLPVYRHGVK 120 Query: 121 RLFLLLLLGILYNHGWGTGAPADPEKVRYASVLGRIAFAWFFAALLVWHTSLRTQVLVAL 180 RLFLLLLLGILYNHGWGTGAP DP+K+RYASVLGRIAFAWFFAALLVWHTSLRTQVLVA+ Sbjct: 121 RLFLLLLLGILYNHGWGTGAPVDPDKIRYASVLGRIAFAWFFAALLVWHTSLRTQVLVAV 180 Query: 181 GILVAYGAVQLWLPFPGGQAGVLSPTESINAYVDSLLLPGVSYQGRTPDPEGVLSTLPAV 240 GILV YGA+QLWLPFPGGQAGVLSPT SINAYVDSLLLPGVSYQGR PDPEGVLSTLPAV Sbjct: 181 GILVGYGAMQLWLPFPGGQAGVLSPTVSINAYVDSLLLPGVSYQGRMPDPEGVLSTLPAV 240 Query: 241 VNALAGVFVGHFIVKSHPKGEWAKVGLLSVAGGVCLALGWLLGGVIPVNKELWTSSFVLV 300 VNALAGVFVGHFIVKSHPKGEWAKVGLL AGGVCLALGWLL VIPVNKELWTSSFVLV Sbjct: 241 VNALAGVFVGHFIVKSHPKGEWAKVGLLGAAGGVCLALGWLLDAVIPVNKELWTSSFVLV 300 Query: 301 TSGWSMLLLALFYALVDVLKWQKLAFIFVVIGTNAIIIYLASSLVDWKYIAQSVFGGVIA 360 TSGWSMLLLALFYALVDVLKWQKL F+FVVIGTNAIIIYLASSLVDWKYIAQSVFGGVIA Sbjct: 301 TSGWSMLLLALFYALVDVLKWQKLVFVFVVIGTNAIIIYLASSLVDWKYIAQSVFGGVIA 360 Query: 361 VLPENAQPLGAVIGLLTVQWLVLYWMYRRNIFVRI 395 VLPE AQPLGAV+ LL VQWLVLYWMYRR IFVRI Sbjct: 361 VLPEYAQPLGAVVSLLNVQWLVLYWMYRRKIFVRI 395 Lambda K H 0.326 0.141 0.457 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 807 Number of extensions: 29 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 395 Length of database: 395 Length adjustment: 31 Effective length of query: 364 Effective length of database: 364 Effective search space: 132496 Effective search space used: 132496 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory