Align histidine ammonia-lyase (EC 4.3.1.3) (characterized)
to candidate 199295 SO0098 histidine ammonia-lyase (NCBI ptt file)
Query= BRENDA::P21310 (510 letters) >FitnessBrowser__MR1:199295 Length = 513 Score = 670 bits (1729), Expect = 0.0 Identities = 349/510 (68%), Positives = 411/510 (80%) Query: 1 MTELTLKPGTLTLAQLRAIHAAPVRLQLDASAAPAIDASVACVEQIIAEDRTAYGINTGF 60 + L L PG+L+LAQLR I + L+L A I+ S V++++ E RT YGINTGF Sbjct: 4 VNHLVLTPGSLSLAQLREISRHKLTLELAPEAINDINISAQIVQKVLDEGRTVYGINTGF 63 Query: 61 GLLASTRIASHDLENLQRSLVLSHAAGIGAPLDDDLVRLIMVLKINSLSRGFSGIRRKVI 120 GLLA+T+IA DL+ LQRS+VLSHAAG G + D VRL+MVLKINSLSRGFSGIR +VI Sbjct: 64 GLLANTKIAPEDLQLLQRSIVLSHAAGTGQYMQDATVRLMMVLKINSLSRGFSGIRLEVI 123 Query: 121 DALIALVNAEVYPHIPLKGSVGASGDLAPLAHMSLVLLGEGKARYKGQWLSATEALAVAG 180 + LI+LVNAEVYP +P KGSVGASGDLAPLAHM L LLGEG+ Y+GQ +SA E L +AG Sbjct: 124 NFLISLVNAEVYPCVPEKGSVGASGDLAPLAHMCLPLLGEGEMSYQGQIISAAEGLEIAG 183 Query: 181 LEPLTLAAKEGLALLNGTQASTAYALRGLFYAEDLYAAAIACGGLSVEAVLGSRSPFDAR 240 L+P+ LAAKEGLALLNGTQASTA AL GLF+AEDL+AA+ G +SVEA +GSRSPFD R Sbjct: 184 LKPIDLAAKEGLALLNGTQASTALALEGLFHAEDLFAASSVIGAMSVEAAMGSRSPFDPR 243 Query: 241 IHEARGQRGQIDTAACFRDLLGDSSEVSLSHKNCDKVQDPYSLRCQPQVMGACLTQLRQA 300 IH ARGQ+GQID+A FR LLG+ SE+SLSH NC+KVQDPYSLRCQPQV+GACLTQ+RQA Sbjct: 244 IHAARGQKGQIDSAMVFRYLLGEESEISLSHANCEKVQDPYSLRCQPQVLGACLTQIRQA 303 Query: 301 AEVLGIEANAVSDNPLVFAAEGDVISGGNFHAEPVAMAADNLALAIAEIGSLSERRISLM 360 AEVL EAN V+DNPLVF GD+ISGGNFHAEPVAMAADNLA+AIAE+G+++ERRI+L+ Sbjct: 304 AEVLATEANGVTDNPLVFQDTGDIISGGNFHAEPVAMAADNLAIAIAELGAIAERRIALL 363 Query: 361 MDKHMSQLPPFLVENGGVNSGFMIAQVTAAALASENKALSHPHSVDSLPTSANQEDHVSM 420 +D +S+LPPFLV+NGGVNSGFMIAQVTAAALASENK +HP SVDSLPTSANQEDHVSM Sbjct: 364 IDSSLSKLPPFLVKNGGVNSGFMIAQVTAAALASENKTYAHPASVDSLPTSANQEDHVSM 423 Query: 421 APAAGKRLWEMAENTRGVLAIEWLGACQGLDLRKGLKTSAKLEKARQALRSEVAHYDRDR 480 A A +RL +M+ENTRGVLAIE L A QGLD R L S + +A+ LR VA+YD+DR Sbjct: 424 ATFAARRLRDMSENTRGVLAIELLAAAQGLDFRAPLTPSKAVAQAKAELREVVAYYDKDR 483 Query: 481 FFAPDIEKAVELLAKGSLTGLLPAGVLPSL 510 +FAPDIE A +LL S LP GVLPSL Sbjct: 484 YFAPDIEAATDLLYTASFNAYLPLGVLPSL 513 Lambda K H 0.318 0.132 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 722 Number of extensions: 21 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 510 Length of database: 513 Length adjustment: 35 Effective length of query: 475 Effective length of database: 478 Effective search space: 227050 Effective search space used: 227050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
Align candidate 199295 SO0098 (histidine ammonia-lyase (NCBI ptt file))
to HMM TIGR01225 (hutH: histidine ammonia-lyase (EC 4.3.1.3))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01225.hmm # target sequence database: /tmp/gapView.22678.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01225 [M=506] Accession: TIGR01225 Description: hutH: histidine ammonia-lyase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.7e-219 714.2 4.2 4.2e-219 714.1 4.2 1.0 1 lcl|FitnessBrowser__MR1:199295 SO0098 histidine ammonia-lyase ( Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__MR1:199295 SO0098 histidine ammonia-lyase (NCBI ptt file) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 714.1 4.2 4.2e-219 4.2e-219 2 502 .. 8 509 .. 7 513 .] 0.99 Alignments for each domain: == domain 1 score: 714.1 bits; conditional E-value: 4.2e-219 TIGR01225 2 vldgesltledleavarekarvelsaaaeeavaksravieeivaedktvYGvntGFGklasvkidkedlaeLqrnlvrs 80 vl ++sl+l++l+++ r+k +el+ +a + ++ s+++++++++e +tvYG+ntGFG la++ki+ edl+ Lqr++v+s lcl|FitnessBrowser__MR1:199295 8 VLTPGSLSLAQLREISRHKLTLELAPEAINDINISAQIVQKVLDEGRTVYGINTGFGLLANTKIAPEDLQLLQRSIVLS 86 78999************************************************************************** PP TIGR01225 81 HaaGvGepleeevvRallvlrlnslakGysgvraevlellvallnaevlPvvpekGsvGasGDLAPLahlalvliGeGe 159 HaaG G+++++++vR+++vl++nsl++G+sg+r ev++ l+ l+naev+P vpekGsvGasGDLAPLah+ l l+GeGe lcl|FitnessBrowser__MR1:199295 87 HAAGTGQYMQDATVRLMMVLKINSLSRGFSGIRLEVINFLISLVNAEVYPCVPEKGSVGASGDLAPLAHMCLPLLGEGE 165 ******************************************************************************* PP TIGR01225 160 aefegevmdaaeaLaaaglePvtlkakEGlALinGtqlmtalavlalvdaekllesadiaaalsleallgtskafdpdi 238 + ++g++++aae L+ agl+P+ l+akEGlAL+nGtq++tala+++l++ae+l +++++++a+s+ea++g++++fdp+i lcl|FitnessBrowser__MR1:199295 166 MSYQGQIISAAEGLEIAGLKPIDLAAKEGLALLNGTQASTALALEGLFHAEDLFAASSVIGAMSVEAAMGSRSPFDPRI 244 ******************************************************************************* PP TIGR01225 239 hevrphrgqiavaarlrella.gseiaeshkdedrvqDaYslRciPqvhGavldtldqvkevlaiElnsatDnPlvfad 316 h++r+++gqi++a +r ll +sei+ sh ++++vqD+YslRc+Pqv Ga+l++++q++evla+E+n +tDnPlvf+d lcl|FitnessBrowser__MR1:199295 245 HAARGQKGQIDSAMVFRYLLGeESEISLSHANCEKVQDPYSLRCQPQVLGACLTQIRQAAEVLATEANGVTDNPLVFQD 323 ********************989******************************************************** PP TIGR01225 317 egevvsgGnFHgepvAlaldflaiaiaelgaiseRRierlldpalseLppFLaedsGlnsGlmiaqytaAaLvsenkaL 395 +g+++sgGnFH+epvA+a+d+laiaiaelgai+eRRi++l+d +ls+LppFL++++G+nsG+miaq+taAaL+senk+ lcl|FitnessBrowser__MR1:199295 324 TGDIISGGNFHAEPVAMAADNLAIAIAELGAIAERRIALLIDSSLSKLPPFLVKNGGVNSGFMIAQVTAAALASENKTY 402 ******************************************************************************* PP TIGR01225 396 ahPasvDsiptsanqEDHvsmgaaaarkllevvenvrrviaiEllaaaqglefrkaektaaelekvyeavRevveelee 474 ahPasvDs+ptsanqEDHvsm++ aar+l+++ en+r v+aiEllaaaqgl+fr++ + + +++++ +++Revv+ +++ lcl|FitnessBrowser__MR1:199295 403 AHPASVDSLPTSANQEDHVSMATFAARRLRDMSENTRGVLAIELLAAAQGLDFRAPLTPSKAVAQAKAELREVVAYYDK 481 ******************************************************************************* PP TIGR01225 475 DRvlapDleavkellekesleaaveakv 502 DR++apD+ea+++ll + s++a ++ v lcl|FitnessBrowser__MR1:199295 482 DRYFAPDIEAATDLLYTASFNAYLPLGV 509 ***************9999988777665 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (506 nodes) Target sequences: 1 (513 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.02s 00:00:00.04 Elapsed: 00:00:00.03 # Mc/sec: 7.66 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory