Align Imidazolonepropionase (EC 3.5.2.7) (characterized)
to candidate 199292 SO0095 imidazolonepropionase (NCBI ptt file)
Query= reanno::MR1:199292 (408 letters) >FitnessBrowser__MR1:199292 Length = 408 Score = 814 bits (2103), Expect = 0.0 Identities = 408/408 (100%), Positives = 408/408 (100%) Query: 1 MSWDQVWIDVNVATMDPSISAPYGAITHAAIAVKDGKIAWLGPRSELPAFDVLSIPVYRG 60 MSWDQVWIDVNVATMDPSISAPYGAITHAAIAVKDGKIAWLGPRSELPAFDVLSIPVYRG Sbjct: 1 MSWDQVWIDVNVATMDPSISAPYGAITHAAIAVKDGKIAWLGPRSELPAFDVLSIPVYRG 60 Query: 61 KGGWITPGLIDAHTHLVFAGNRANEFELRLKGATYEEIARAGGGIISTVNACREADEAAL 120 KGGWITPGLIDAHTHLVFAGNRANEFELRLKGATYEEIARAGGGIISTVNACREADEAAL Sbjct: 61 KGGWITPGLIDAHTHLVFAGNRANEFELRLKGATYEEIARAGGGIISTVNACREADEAAL 120 Query: 121 FELGRQRLNALAKEGVTTVEIKSGYGLDTETELKILRVARELGKHHHVDVKTTFLGAHAV 180 FELGRQRLNALAKEGVTTVEIKSGYGLDTETELKILRVARELGKHHHVDVKTTFLGAHAV Sbjct: 121 FELGRQRLNALAKEGVTTVEIKSGYGLDTETELKILRVARELGKHHHVDVKTTFLGAHAV 180 Query: 181 PPEYKGNSDGYVDLIINKMLPAVIKENLADAVDVFCENIAFNLEQTERVLSAAKAAGLQV 240 PPEYKGNSDGYVDLIINKMLPAVIKENLADAVDVFCENIAFNLEQTERVLSAAKAAGLQV Sbjct: 181 PPEYKGNSDGYVDLIINKMLPAVIKENLADAVDVFCENIAFNLEQTERVLSAAKAAGLQV 240 Query: 241 KLHAEQLSNMGGSELAARLGAKSVDHIEYLDEAGVKALSESGTCAVLLPGAFYFLRETQK 300 KLHAEQLSNMGGSELAARLGAKSVDHIEYLDEAGVKALSESGTCAVLLPGAFYFLRETQK Sbjct: 241 KLHAEQLSNMGGSELAARLGAKSVDHIEYLDEAGVKALSESGTCAVLLPGAFYFLRETQK 300 Query: 301 PPIDLLRQYGVPMVLASDFNPGSFPICSTLLMLNMGCTLFRLTPEEALAGLTLNAAKALG 360 PPIDLLRQYGVPMVLASDFNPGSFPICSTLLMLNMGCTLFRLTPEEALAGLTLNAAKALG Sbjct: 301 PPIDLLRQYGVPMVLASDFNPGSFPICSTLLMLNMGCTLFRLTPEEALAGLTLNAAKALG 360 Query: 361 IEDKVGSLVVGKQADFCLWNIATPAQLAYSYGVNPCKDVVKNGKLVHQ 408 IEDKVGSLVVGKQADFCLWNIATPAQLAYSYGVNPCKDVVKNGKLVHQ Sbjct: 361 IEDKVGSLVVGKQADFCLWNIATPAQLAYSYGVNPCKDVVKNGKLVHQ 408 Lambda K H 0.319 0.136 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 728 Number of extensions: 9 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 408 Length of database: 408 Length adjustment: 31 Effective length of query: 377 Effective length of database: 377 Effective search space: 142129 Effective search space used: 142129 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate 199292 SO0095 (imidazolonepropionase (NCBI ptt file))
to HMM TIGR01224 (hutI: imidazolonepropionase (EC 3.5.2.7))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01224.hmm # target sequence database: /tmp/gapView.8927.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01224 [M=377] Accession: TIGR01224 Description: hutI: imidazolonepropionase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.8e-152 492.4 0.0 4.4e-152 492.2 0.0 1.0 1 lcl|FitnessBrowser__MR1:199292 SO0095 imidazolonepropionase (NC Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__MR1:199292 SO0095 imidazolonepropionase (NCBI ptt file) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 492.2 0.0 4.4e-152 4.4e-152 3 376 .. 28 404 .. 26 405 .. 0.98 Alignments for each domain: == domain 1 score: 492.2 bits; conditional E-value: 4.4e-152 TIGR01224 3 daailveegkiaaigqkaalpgeeaakiidl..eGklvvPGlvDpHtHlvfagdRvkefelklqGasYleilaeGgGil 79 +aai+v++gkia++g++++lp+ + + i + +G+ ++PGl+D+HtHlvfag+R++efel+l+Ga+Y+ei+++GgGi+ lcl|FitnessBrowser__MR1:199292 28 HAAIAVKDGKIAWLGPRSELPAFDVLSIPVYrgKGGWITPGLIDAHTHLVFAGNRANEFELRLKGATYEEIARAGGGII 106 689*******************987776655226789****************************************** PP TIGR01224 80 stvratraAseeellkeaeerlkkllrsGtttlEvKsGYGLdleaElkmLrvikalkeelpvdvvttflgaHavPkevq 158 stv+a r+A+e++l++ ++rl+ l ++G+tt+E+KsGYGLd+e+Elk+Lrv+++l ++++vdv+ttflgaHavP+e++ lcl|FitnessBrowser__MR1:199292 107 STVNACREADEAALFELGRQRLNALAKEGVTTVEIKSGYGLDTETELKILRVARELGKHHHVDVKTTFLGAHAVPPEYK 185 ******************************************************************************* PP TIGR01224 159 ededeyvdaileelipkvaeeklaeavDvFcekgvFsveqsrrilkaaqeaGlavklHaeelkalggaelaaklgavsa 237 +++d yvd i+++++p+v +e+la+avDvFce+ +F+ eq++r+l+aa++aGl+vklHae+l+++gg+elaa+lga s+ lcl|FitnessBrowser__MR1:199292 186 GNSDGYVDLIINKMLPAVIKENLADAVDVFCENIAFNLEQTERVLSAAKAAGLQVKLHAEQLSNMGGSELAARLGAKSV 264 ******************************************************************************* PP TIGR01224 238 dHleeasdedikalaeagtvavlLPgtaflLr.eaapparklidekvivalatDlnPgsspllslqlilslavtllklt 315 dH+e+++++++kal e+gt avlLPg++++Lr +++pp++ l++++v++ la+D+nPgs p+ s+ l+l++++tl++lt lcl|FitnessBrowser__MR1:199292 265 DHIEYLDEAGVKALSESGTCAVLLPGAFYFLReTQKPPIDLLRQYGVPMVLASDFNPGSFPICSTLLMLNMGCTLFRLT 343 ********************************999******************************************** PP TIGR01224 316 aeealaaatvnaAqalglgeekGtleeGkdadlvvlsaesyeeiaYrlgvnvveaviknGe 376 +eeala+ t+naA+alg +++ G+l Gk+ad+ ++++ ++++aY +gvn + v+knG+ lcl|FitnessBrowser__MR1:199292 344 PEEALAGLTLNAAKALGIEDKVGSLVVGKQADFCLWNIATPAQLAYSYGVNPCKDVVKNGK 404 ************************************************************7 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (377 nodes) Target sequences: 1 (408 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 7.35 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory