Align MalK; aka Sugar ABC transporter, ATP-binding protein, component of The maltose, maltotriose, mannotetraose (MalE1)/maltose, maltotriose, trehalose (MalE2) porter (Nanavati et al., 2005). For MalG1 (823aas) and MalG2 (833aas), the C-terminal transmembrane domain with 6 putative TMSs is preceded by a single N-terminal TMS and a large (600 residue) hydrophilic region showing sequence similarity to MLP1 and 2 (9.A.14; e-12 & e-7) as well as other proteins (characterized)
to candidate 200449 SO1271 polyamine ABC transporter, ATP-binding protein (NCBI ptt file)
Query= TCDB::Q9X103 (369 letters) >FitnessBrowser__MR1:200449 Length = 378 Score = 234 bits (597), Expect = 3e-66 Identities = 143/336 (42%), Positives = 203/336 (60%), Gaps = 24/336 (7%) Query: 8 LENVTKVYENKVVAVKNANLVVEDKEFVVLLGPSGCGKTTTLRMIAGLEEITDGKIYIDG 67 +E V+K++++ V AV + +L + E LLG SG GK+T LRM+AG E T G+IY+DG Sbjct: 23 IERVSKLFDD-VRAVDDVSLTINKGEIFALLGGSGSGKSTLLRMLAGFERPTSGRIYLDG 81 Query: 68 KVVNDVEPKDRDIAMVFQNYALYPHMTVYENMAFGLKLRKYPKDEIDRRVREAAKILGIE 127 + + D+ P +R I M+FQ+YAL+PHMTV +N+AFGLK K PK EI++RV+E K++ +E Sbjct: 82 EDITDLPPYERPINMMFQSYALFPHMTVAQNIAFGLKQDKLPKAEIEQRVQEMLKLVHME 141 Query: 128 NLLDRKPRQLSGGQRQRVAVGRAIVRNPKVFLFDEPLSNLDAKLRVQMRSELKKLHHRLQ 187 RKP QLSGGQRQRVA+ R++ + PK+ L DEP+ LD KLR QM+ E+ ++ R+ Sbjct: 142 QYGKRKPHQLSGGQRQRVALARSLAKRPKLLLLDEPMGALDKKLRTQMQLEVVEILERVG 201 Query: 188 ATIIYVTHDQVEAMTMADKIVVMKDGEIQQIGTPHEIYNSPANVFVAGFIGSPPMNFVNA 247 T + VTHDQ EAMTMA +I +M DG I Q G+P +IY SP + +A FIGS +N + Sbjct: 202 VTCVMVTHDQEEAMTMAGRISIMSDGWIAQTGSPMDIYESPNSRMIAEFIGS--VNLFSG 259 Query: 248 RVVRGEGGLWI-----QASGFKV--KVPKEFEDK---LANYIDKEIIFGIRPEDIYDKLF 297 + E I A F V V EDK LA +K II +PE Y Sbjct: 260 EIEVDEVDHLIIKPNDLARSFYVGYGVTTSLEDKRVWLAVRPEKTIISREQPEGEY---- 315 Query: 298 ALAPSPENTITGVVDVVEPLGSETILHVKVGDDLIV 333 N G+V + LG ++ ++++ + IV Sbjct: 316 -------NWAKGIVHDIAYLGGISVYYIRLENGQIV 344 Lambda K H 0.319 0.138 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 347 Number of extensions: 12 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 369 Length of database: 378 Length adjustment: 30 Effective length of query: 339 Effective length of database: 348 Effective search space: 117972 Effective search space used: 117972 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory