Align α-glucosidase (SusB;Aglu;BFO_2272) (EC 3.2.1.20) (characterized)
to candidate 201594 SO2450 alpha-glucosidase (NCBI ptt file)
Query= CAZy::AEW20125.1 (708 letters) >FitnessBrowser__MR1:201594 Length = 699 Score = 492 bits (1267), Expect = e-143 Identities = 281/708 (39%), Positives = 397/708 (56%), Gaps = 66/708 (9%) Query: 15 SFLVSAPFTMRAEELKSPDGNLLMRFEVNGEGTPVYELTYKRKPVIKPSRLGLEIEGFS- 73 S SA + L SP+ ++ + + G Y++ + K +I+PSRLGL + Sbjct: 33 SSFFSANLVAQTVTLSSPNQHIQISLS-DDTGRAEYKIDFHGKTIIEPSRLGLVFQSIGE 91 Query: 74 LRKAFTLKGVETVTFDETWTPVWGEVKAIRNHYNEMTATLHQADGDRTMVIRFRLYDDGL 133 L + +K + D+ W WGE + I++ +N+++ L DG + + F +DDG+ Sbjct: 92 LGQGLRIKSSHITSTDDKWQQPWGEQEWIKDQHNQLSVVL--TDGKIDLNLEFNAFDDGI 149 Query: 134 GFRYEFPQQKSLNY---FVIKEEYSEFAMAGDHKA--FWIPG-DYDTQEYDYTESRLTEI 187 GFRY +Q +L I +E +EF + +KA +WIP ++ EY Y S L ++ Sbjct: 150 GFRYHLAEQAALPVDTPLSITDELTEFNIGQSNKATAWWIPSRGWNRYEYLYRTSALNQV 209 Query: 188 RGLMQTAITPNTSQTPFSPTGVQTALMMKSDDGLYINLHEAALVDYPCMSLNLDDQTLTF 247 + + TPF+ + DG ++++HEAALV++ M+L+ + Sbjct: 210 ----------DRAHTPFT---------FRLADGTHLSIHEAALVNFASMTLD-QQRDGKL 249 Query: 248 QSWLTPDAQGKKGYMQTPCHTPWRTVIVSDDARRILASHLTLNLNEPCKLDDVSWIRPVK 307 ++ LTP + G + Q +TPWRT+ ++D A +L SHL LNLNEP KL DVSWI+P K Sbjct: 250 KADLTPWSDGIRVKTQAGFYTPWRTIQIADKATGLLNSHLILNLNEPNKLGDVSWIKPGK 309 Query: 308 YIGVWWEMISGKSTWAYTNDLPSVHLDVTDYTKMKPNGTHAANNEKVRRYIDFAAKHGFD 367 Y+G+WW M K+TW H A + +RY+DFAAK+ FD Sbjct: 310 YVGIWWGMHLNKNTWG-------------------SGPKHGATTSETKRYMDFAAKYHFD 350 Query: 368 QVLVEGWNIGWE-DWFGNSKDYVFDFVTPYPDFDLKALNEYAHSKGVKLMMHHETSSSVR 426 VLVEGWN GW+ DWF N F F Y DFDL A+ Y KGV+L+ HHET+ SV Sbjct: 351 GVLVEGWNQGWDGDWFHNGDQ--FSFTKAYDDFDLPAITAYGAKKGVRLIGHHETAGSVT 408 Query: 427 NYERQMEKAYKLMNDYGYNAVKSGYVG----------NIIPRGEYHYGQWMVNHYLYAVK 476 +Y +QM A+ L +G VK+GYV N I R E+H GQ+MV YLY V Sbjct: 409 HYRKQMADAFILYEKHGVTQVKTGYVADGGQIKRFDENGIMRHEWHDGQFMVGEYLYNVT 468 Query: 477 KAAEHRIMVNAHEAVRPTGLCRTYPNLIGNEAARGGEYESFGG--NKVFHTTILPFTRLM 534 +AA+H I +N HE ++ TGL RTYPN I E ARG EY ++G N HT +LPFTR++ Sbjct: 469 EAAKHHISINTHEPIKDTGLRRTYPNWISREGARGQEYNAWGSPPNSPEHTAMLPFTRML 528 Query: 535 GGPMDYTPGIFETRLEKVNPGNNSYVHSTLARQLALYVTMYSPLQMAADLPENYERFMDA 594 GPMD+TPGIF+ + ++ N V +TL +QLALYV +YSP+QMAADLP NY++ +DA Sbjct: 529 AGPMDFTPGIFDLAPKGLDAENR--VQTTLMKQLALYVVLYSPIQMAADLPRNYQKHLDA 586 Query: 595 FQFIKDVAIDWDDSKYLEAEPGRYITVARKAKGTNNWFVGCTANEDGHLSNVSLDFLDPG 654 FQFI+DV DW S L E G Y+ AR+AK +W++G +E V LDFLDP Sbjct: 587 FQFIRDVPTDWYQSIALAGEVGDYVVFARQAKDQKDWYLGALTDETARKVTVKLDFLDPH 646 Query: 655 RKYTATIYADAPTAHYEKNPQAYTIKQMKVTNKSKLTLRAASGGGYAI 702 ++Y A IY D A ++ NP Y I+ +V+ K L L AS G AI Sbjct: 647 KRYQAQIYRDGAKADWKTNPYDYVIETKEVSAKDSLNLALASSSGTAI 694 Lambda K H 0.319 0.135 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1427 Number of extensions: 68 Number of successful extensions: 10 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 708 Length of database: 699 Length adjustment: 39 Effective length of query: 669 Effective length of database: 660 Effective search space: 441540 Effective search space used: 441540 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory