Aligments for a candidate for pad-dh in Shewanella oneidensis MR-1

GapMind for catabolism of small carbon sources

Aligments for a candidate for pad-dh in Shewanella oneidensis MR-1

Align aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) (characterized)
to candidate 202865 SO3774 proline dehydrogenase/delta-1-pyrroline-5-carboxylate dehydrogenase, putative (NCBI ptt file)

Query= BRENDA::P05091
         (517 letters)



>lcl|FitnessBrowser__MR1:202865 SO3774 proline
            dehydrogenase/delta-1-pyrroline-5-carboxylate
            dehydrogenase, putative (NCBI ptt file)
          Length = 1059

 Score =  191 bits (486), Expect = 9e-53
 Identities = 141/448 (31%), Positives = 222/448 (49%), Gaps = 23/448 (5%)

Query: 61   TGEVICQVAEGDKEDVDKAVKAARAAFQLGSPWRRMDASHRGRLLNRLADLIERDRTYLA 120
            T + + QVA  DK  +++AV +A AAF     W R     R   L +LADL+E +R  L 
Sbjct: 587  TTQTVGQVAFADKAAIEQAVSSAHAAF---GSWTRTPVEVRASALQKLADLLEENREELI 643

Query: 121  ALETLDNGKPYVISYLVDLDMVLKCLRYYAGWADKYHGKTIPIDGDFFS----YTRHEPV 176
            AL T + GK  +   + ++   +   RYYA  A K   K   + G        + +   V
Sbjct: 644  ALCTREAGKS-IQDGIDEVREAVDFCRYYAVQAKKLMSKPELLPGPTGELNELFLQGRGV 702

Query: 177  GVCGQIIPWNFPLLMQAWKLGPALATGNVVVMKVAEQTPLTALYVANLIKEAGFPPGVVN 236
             VC  I PWNFPL +   ++  ALA GN VV K AEQT +       L  +AG P  V+ 
Sbjct: 703  FVC--ISPWNFPLAIFLGQVSAALAAGNTVVAKPAEQTSIIGYRAVQLAHQAGIPTDVLQ 760

Query: 237  IVPGFGPTAGAAIASHEDVDKVAFTGSTEIGRVIQ--VAAGSSNLKRVTLELGGKSPNII 294
             +PG G T G A+ + E +  V FTGST   ++I   +A     +  +  E GG++  ++
Sbjct: 761  YLPGTGATVGNALTADERIGGVCFTGSTGTAKLINRTLANREGAIIPLIAETGGQNAMVV 820

Query: 295  MSDADMDWAVEQAHFALFFNQGQCCCAGSRTFVQEDIYDEFVERSVARAKSRVVGNPFDS 354
             S +  +  V     + F + GQ C A    F+QEDI D  ++         V+GNP   
Sbjct: 821  DSTSQPEQVVNDVVSSSFTSAGQRCSALRVLFLQEDIADRVIDVLQGAMDELVIGNPSSI 880

Query: 355  KTEQGPQVDETQFKKILGYINTGKQEGAKLLCGGGIAA--DRGYFIQPTVFGDVQDGMTI 412
            KT+ GP +D T    +  +I+  KQ G KL+    + A  + G+F+ PT      D + +
Sbjct: 881  KTDVGPVIDATAKANLDAHIDHIKQVG-KLIKQMSLPAGTENGHFVAPTAV--EIDSIKV 937

Query: 413  AKEEIFGPVMQILKFKTIE--EVVGRANNSTYGLAAAVFTKDLDKANYLSQALQAGTVWV 470
             ++E FGP++ ++++K  E   V+   N++ +GL   + +++   A  ++  +  G V++
Sbjct: 938  LEKEHFGPILHVIRYKASELAHVIDEINSTGFGLTLGIHSRNEGHALEVADKVNVGNVYI 997

Query: 471  NCYD---VFGAQSPFGGYKMSGSGRELG 495
            N      V G Q PFGG  +SG+G + G
Sbjct: 998  NRNQIGAVVGVQ-PFGGQGLSGTGPKAG 1024


Lambda     K      H
   0.319    0.136    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1121
Number of extensions: 55
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 517
Length of database: 1059
Length adjustment: 40
Effective length of query: 477
Effective length of database: 1019
Effective search space:   486063
Effective search space used:   486063
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources