Alignments for a candidate for putP in Shewanella oneidensis MR-1

GapMind for catabolism of small carbon sources

Alignments for a candidate for putP in Shewanella oneidensis MR-1

Align L-proline uptake porter, PutP (characterized)
to candidate 370215 SO3097.4 hypothetical sodium/proline symporter (NCBI ptt file)

Query= TCDB::Q9I5F5
         (506 letters)



>FitnessBrowser__MR1:370215
          Length = 246

 Score =  206 bits (523), Expect = 1e-57
 Identities = 114/224 (50%), Positives = 146/224 (65%), Gaps = 12/224 (5%)

Query: 183 VSWTDTVQASLMIFALILTPVIVMLATGGVEPTFTAIELKDATSFDMLKG-ASFIGVISL 241
           +SWTD  QASLM+ AL++ P  V         +   IE  D     ++    + IG++SL
Sbjct: 1   MSWTDFFQASLMLIALLIIPFAVFSRP----ESHAGIESIDPAMLALISDKTTMIGLLSL 56

Query: 242 MAWGLGYFGQPHILARFMAADSVKSIPAARRISMTWMILCLGGAVAVGFFGIAYFQAHPE 301
           +AWGLGYFGQPHIL+RFMA  +V ++P +RRI+M+WMIL L GA+A G  G  YF   P 
Sbjct: 57  LAWGLGYFGQPHILSRFMAIGTVDALPTSRRIAMSWMILSLLGALATGLAGSVYFANEP- 115

Query: 302 QAGAVSENPERVFIELAKILFNPWIAGVLLSAILAAVMSTLSCQLLVCSSALTEDFYKAF 361
                  N E VF  LA+  FNPWI G+L++AIL+A+MST+  QLLVCSS +TEDFY+ +
Sbjct: 116 -----LANAETVFFHLAQAAFNPWIGGLLIAAILSAIMSTIDSQLLVCSSVITEDFYRKW 170

Query: 362 LRKGASQLELVWVGRAMVLLVAVIAIWLASNPENRVLGLVSYAW 405
           LR      EL+ VGR  VL +AVIA  +A NPEN VL LVSYAW
Sbjct: 171 LRP-TDDRELMMVGRMGVLAIAVIAGIIALNPENSVLNLVSYAW 213


Lambda     K      H
   0.326    0.138    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 370
Number of extensions: 17
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 506
Length of database: 246
Length adjustment: 29
Effective length of query: 477
Effective length of database: 217
Effective search space:   103509
Effective search space used:   103509
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 49 (23.5 bits)

Align L-proline uptake porter, PutP (characterized)
to candidate 370285 SO3097.3 hypothetical sodium/proline symporter (NCBI ptt file)

Query= TCDB::Q9I5F5
         (506 letters)



>FitnessBrowser__MR1:370285
          Length = 115

 Score =  137 bits (346), Expect = 1e-37
 Identities = 67/114 (58%), Positives = 82/114 (71%)

Query: 68  VYLSGLSESWIAIGLIVGAYLNWLFVAGRLRVQTEHNGNALTLPDYFTNRFEDNSRLLRI 127
           +YL GL E+WI IGL++GA+LN LFVA RLR+ T+   NALTLPD+F  RF D    L++
Sbjct: 1   MYLGGLEEAWIGIGLVIGAWLNRLFVAKRLRIYTQLADNALTLPDFFEKRFHDKQGYLKL 60

Query: 128 FSALVILVFFTIYCASGIVAGARLFESTFGLSYETALWAGAAATIAYTFIGGFL 181
            SA+ ILVFFT Y +SG+V GA LFE  FGL Y  AL  G+A  + YTFIGGFL
Sbjct: 61  VSAVTILVFFTFYASSGMVGGAILFEKVFGLDYNVALVIGSAIIVGYTFIGGFL 114


Lambda     K      H
   0.326    0.138    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 225
Number of extensions: 12
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 506
Length of database: 115
Length adjustment: 23
Effective length of query: 483
Effective length of database: 92
Effective search space:    44436
Effective search space used:    44436
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources