Align aconitate hydratase (EC 4.2.1.3) (characterized)
to candidate 199624 SO0432 aconitate hydratase 2 (NCBI ptt file)
Query= BRENDA::P36683 (865 letters) >FitnessBrowser__MR1:199624 Length = 865 Score = 1437 bits (3720), Expect = 0.0 Identities = 700/865 (80%), Positives = 784/865 (90%) Query: 1 MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEFLLDLLTNRVPPGVDEA 60 MLE YRKHVAERAAEG+ PKPLDA+Q+A LV+L++NPPAGEE F+LDLL NR+PPGVDEA Sbjct: 1 MLEAYRKHVAERAAEGVVPKPLDAHQVAELVKLVQNPPAGEEAFVLDLLENRIPPGVDEA 60 Query: 61 AYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPLIDALDDAKLAPIAAKALS 120 AYVKA FL A+AKG A SP+L+ E+A ELLGTMQGGYNI PLI LD+ LAP+AAKAL+ Sbjct: 61 AYVKAAFLDAVAKGTATSPILSAERATELLGTMQGGYNIEPLIAQLDNPALAPLAAKALA 120 Query: 121 HTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRPALAEKLTVTVFKVTGETNTD 180 HTLLMFD+F+DV EK +AGN++AKQV+++WA+A+W+L+RP LA+K+T+TVFKV+GETNTD Sbjct: 121 HTLLMFDSFHDVVEKMEAGNQFAKQVVEAWANADWYLSRPKLADKVTLTVFKVSGETNTD 180 Query: 181 DLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVGPIKQIEALQQKGFPLAYVGDVV 240 DLSPAPDAWSRPDIPLHALAMLKNAR+GIEPD G VGPIK++E L+ KGFPL YVGDVV Sbjct: 181 DLSPAPDAWSRPDIPLHALAMLKNARDGIEPDVAGSVGPIKKLEELKTKGFPLVYVGDVV 240 Query: 241 GTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGGKIAPIFFNTMEDAGALPIEVDVSN 300 GTGSSRKSATNSVLWFMGDDIP VPNKR GG CLGGKIAPIFFNTMEDAGALPIE+DVS Sbjct: 241 GTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIELDVSK 300 Query: 301 LNMGDVIDVYPYKGEVRNHETGELLATFELKTDVLIDEVRAGGRIPLIIGRGLTTKAREA 360 + MGDVID+YPY G V+ H + E+++ F LKTDVL+DEVRAGGRIPLIIGRGLT KAR Sbjct: 301 MEMGDVIDIYPYAGVVKRHGSDEVISEFSLKTDVLLDEVRAGGRIPLIIGRGLTDKARAV 360 Query: 361 LGLPHSDVFRQAKDVAESDRGFSLAQKMVGRACGVKGIRPGAYCEPKMTSVGSQDTTGPM 420 LGLP S+VF + +D+A++ +G++LAQKMVG+ACGV G+RPG YCEPKMTSVGSQDTTGPM Sbjct: 361 LGLPASEVFVRPQDIADNGKGYTLAQKMVGKACGVAGVRPGQYCEPKMTSVGSQDTTGPM 420 Query: 421 TRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGVSLRPGDGVIH 480 TRDELKDLACLGFSADL MQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGVSLRPGDGVIH Sbjct: 421 TRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGVSLRPGDGVIH 480 Query: 481 SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKM 540 SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKM Sbjct: 481 SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKM 540 Query: 541 QPGITLRDLVHAIPLYAIKQGLLTVEKKGKKNIFSGRILEIEGLPDLKVEQAFELTDASA 600 QPGITLRDLVHAIPL AI+ G+LTVEKKGK NIFSGR+LEIEGL LKVEQAFEL+DASA Sbjct: 541 QPGITLRDLVHAIPLKAIELGMLTVEKKGKVNIFSGRVLEIEGLESLKVEQAFELSDASA 600 Query: 601 ERSAAGCTIKLNKEPIIEYLNSNIVLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEA 660 ERSAAGCTIKL+KEPIIEYLNSNI +LKWMIAEGYGDRRT+ERRI+GME+WLANPEL+ A Sbjct: 601 ERSAAGCTIKLDKEPIIEYLNSNITMLKWMIAEGYGDRRTIERRIKGMEEWLANPELMSA 660 Query: 661 DADAEYAAVIDIDLADIKEPILCAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAA 720 DADAEY AVI+IDL +IKEPILCAPNDPDDA LS+V KIDEVF+GSCMTNIGHFRA Sbjct: 661 DADAEYTAVIEIDLNEIKEPILCAPNDPDDAVLLSSVAQTKIDEVFVGSCMTNIGHFRAT 720 Query: 721 GKLLDAHKGQLPTRLWVAPPTRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVA 780 GK+LD LPTRLW+APPT+MD QLTEEGYY +FG+ GARIEIPGCSLCMGNQARVA Sbjct: 721 GKMLDKFAKTLPTRLWIAPPTKMDKDQLTEEGYYGIFGRVGARIEIPGCSLCMGNQARVA 780 Query: 781 DGATVVSTSTRNFPNRLGTGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDT 840 +GATVVSTSTRNFPNRLGTGANV+LASAELAAVAAL+G+LPT EEYQ Y ++D TA DT Sbjct: 781 EGATVVSTSTRNFPNRLGTGANVYLASAELAAVAALLGRLPTVEEYQEYAKELDATAADT 840 Query: 841 YRYLNFNQLSQYTEKADGVIFQTAV 865 YRYLNF+Q+ YT+KA VIFQ+AV Sbjct: 841 YRYLNFDQIDSYTKKASQVIFQSAV 865 Lambda K H 0.317 0.136 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 2130 Number of extensions: 81 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 865 Length of database: 865 Length adjustment: 42 Effective length of query: 823 Effective length of database: 823 Effective search space: 677329 Effective search space used: 677329 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 56 (26.2 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory