Align UTP--glucose-1-phosphate uridylyltransferase; EC 2.7.7.9 (characterized)
to candidate 200830 SO1665 UTP-glucose-1-phosphate uridylyltransferase (NCBI ptt file)
Query= CharProtDB::CH_021667 (302 letters) >FitnessBrowser__MR1:200830 Length = 302 Score = 408 bits (1048), Expect = e-119 Identities = 210/298 (70%), Positives = 242/298 (81%), Gaps = 3/298 (1%) Query: 7 KVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQYVVNECIAAGITEIVLVTHSSKN 66 +++KAVIPVAGLGTRMLPATKAIPKEMLP+VDKPLIQYVV+E IAAGI EIVLVTH+SKN Sbjct: 5 QIRKAVIPVAGLGTRMLPATKAIPKEMLPVVDKPLIQYVVSEAIAAGIKEIVLVTHASKN 64 Query: 67 SIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHVTIMQVRQGLAKGLGHAVLCAHPVV 126 SIENHFDTSFELEA LE+RVKRQLL+ VQSICP VT++ VRQ AKGLGHA+LCA VV Sbjct: 65 SIENHFDTSFELEAQLERRVKRQLLEAVQSICPKDVTVISVRQSQAKGLGHAILCAKSVV 124 Query: 127 GDEPVAVILPDVILDEYESDLSQDNLAEMIRRFDETGHSQIMVEPVAD--VTAYGVVDCK 184 GD P AV+LPDVI+DE DL DNLA M+ FDET QIMVE V V YG+ D Sbjct: 125 GDAPFAVLLPDVIIDEASCDLKTDNLASMVSLFDETQVGQIMVEGVPHHLVNQYGIADVN 184 Query: 185 GVELAPGESVPMVGVVEKPKADVAPSNLAIVGRYVLSADIWPLLAKTPPGAGDEIQLTDA 244 G +L PGES P+V +VEKP D APSNLA+VGRYVL A IWPLLAKTP GAGDEIQLTDA Sbjct: 185 GHDLQPGESEPLVELVEKPPVDEAPSNLAVVGRYVLPAAIWPLLAKTPAGAGDEIQLTDA 244 Query: 245 IDMLIEKETVEAYHMKGKSHDCGNKLGYMQAFVEYGIRHNTLGTEFKAWLEEEM-GIK 301 I ML+++ETV AY+M+GKSHDCGNK GYM+A VEY +RH+ +G +F +L+ + GIK Sbjct: 245 IAMLMKEETVNAYYMQGKSHDCGNKQGYMRANVEYALRHSEIGEDFAHYLKTVVKGIK 302 Lambda K H 0.317 0.135 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 367 Number of extensions: 14 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 302 Length of database: 302 Length adjustment: 27 Effective length of query: 275 Effective length of database: 275 Effective search space: 75625 Effective search space used: 75625 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 48 (23.1 bits)
Align candidate 200830 SO1665 (UTP-glucose-1-phosphate uridylyltransferase (NCBI ptt file))
to HMM TIGR01099 (galU: UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01099.hmm # target sequence database: /tmp/gapView.10994.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01099 [M=261] Accession: TIGR01099 Description: galU: UTP--glucose-1-phosphate uridylyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.8e-114 367.7 0.1 2.1e-114 367.5 0.1 1.0 1 lcl|FitnessBrowser__MR1:200830 SO1665 UTP-glucose-1-phosphate u Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__MR1:200830 SO1665 UTP-glucose-1-phosphate uridylyltransferase (NCBI ptt file) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 367.5 0.1 2.1e-114 2.1e-114 1 261 [] 6 275 .. 6 275 .. 0.97 Alignments for each domain: == domain 1 score: 367.5 bits; conditional E-value: 2.1e-114 TIGR01099 1 irkaviPaaGlGtrlLPatkaiPkemlpivdkPliqyvveeaveaGieeivlvtgrskraiedhfDtsyeleaklekkn 79 irkaviP+aGlGtr+LPatkaiPkemlp+vdkPliqyvv ea++aGi+eivlvt+ sk++ie+hfDts+elea+le++ lcl|FitnessBrowser__MR1:200830 6 IRKAVIPVAGLGTRMLPATKAIPKEMLPVVDKPLIQYVVSEAIAAGIKEIVLVTHASKNSIENHFDTSFELEAQLERRV 84 89***************************************************************************** PP TIGR01099 80 keellkevrkiael.atilyvrqkeakGLGhavllaeelvgdepfavllgDdlvseeee.....alkqlielyektgas 152 k++ll+ v++i+++ +t++ vrq +akGLGha+l+a+++vgd pfavll+D++++e + +l+ +++l+++t+ lcl|FitnessBrowser__MR1:200830 85 KRQLLEAVQSICPKdVTVISVRQSQAKGLGHAILCAKSVVGDAPFAVLLPDVIIDEASCdlktdNLASMVSLFDETQVG 163 *************99***************************************99876677779************** PP TIGR01099 153 iiaveevpkeevskYGvidgeeveeelye...vkdlvekPkpeeapsnlaivGrYvltpeifelleetkaGkggeiqlt 228 +i+ve vp++ v++YG++d+++ + + e + +lvekP ++eapsnla+vGrYvl ++i+ ll++t+aG+g+eiqlt lcl|FitnessBrowser__MR1:200830 164 QIMVEGVPHHLVNQYGIADVNGHDLQPGEsepLVELVEKPPVDEAPSNLAVVGRYVLPAAIWPLLAKTPAGAGDEIQLT 242 ********************99888877666799********************************************* PP TIGR01099 229 DalrlllekeevlavklkgkryDvGdklgylka 261 Da+++l+++e+v a+ ++gk++D+G+k+gy++a lcl|FitnessBrowser__MR1:200830 243 DAIAMLMKEETVNAYYMQGKSHDCGNKQGYMRA 275 *******************************87 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (261 nodes) Target sequences: 1 (302 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00 # Mc/sec: 9.27 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory