GapMind for catabolism of small carbon sources

 

Aligments for a candidate for glk in Shewanella oneidensis MR-1

Align glucokinase (EC 2.7.1.2) (characterized)
to candidate 200821 SO1656 ROK family protein (NCBI ptt file)

Query= reanno::SB2B:6938110
         (299 letters)



>lcl|FitnessBrowser__MR1:200821 SO1656 ROK family protein (NCBI ptt
           file)
          Length = 306

 Score =  497 bits (1279), Expect = e-145
 Identities = 238/306 (77%), Positives = 268/306 (87%), Gaps = 8/306 (2%)

Query: 1   MMRMGVDLGGTKIELVALGEDGSELFRKRIATPREYQGTLNAVVTLVNEAEATLGTQGSL 60
           M+RMG+DLGGTKIELVAL  +G+E+ RKRI TPR+YQGTLNA+V LVNEAE+TLG +GS+
Sbjct: 1   MIRMGIDLGGTKIELVALNNEGNEVVRKRINTPRDYQGTLNAIVDLVNEAESTLGEKGSV 60

Query: 61  GIGIPGVISPYTGLVKNANSTWINGHPLDRDLGALLNREVRVANDANCFAVSEAVDGAAA 120
           G+GIPGVISPY+GLVKNANSTWINGHPLD  LG LL REVRVANDANCFA+SEAVDGAAA
Sbjct: 61  GVGIPGVISPYSGLVKNANSTWINGHPLDVHLGELLGREVRVANDANCFALSEAVDGAAA 120

Query: 121 GKRVVFGAILGTGCGAGLAFDGRVHEGGNGIGGEWGHNPLPWMRPDEFNTTECFCGNKDC 180
           GK VVFG I+GTGCGAG+A +G+VH GGNGIGGEWGHNPLPWM  +EFNTT CFCGN DC
Sbjct: 121 GKSVVFGVIIGTGCGAGVAINGKVHAGGNGIGGEWGHNPLPWMTKEEFNTTRCFCGNPDC 180

Query: 181 IETFVSGTGFVRDFRNSGG--------TAQNGAEIMSLVDAGDELANLVFDRYLDRLARS 232
           IETF+SGTGFVRD+  +           A++G+EIMSLVD GDE+A   F+RY+DRLARS
Sbjct: 181 IETFISGTGFVRDYNEALSRAASVQRVPAKSGSEIMSLVDGGDEIALAAFERYVDRLARS 240

Query: 233 LAHVINMLDPDAIVLGGGMSNVQAIYARLPAILPKYVVGRECRTPVVQNLYGCSSGVRGA 292
           LAHVIN+LDPDAIVLGGGMSNV+AIY RLPA+L  YVVGRECRTPVVQNLYGCSSGVRGA
Sbjct: 241 LAHVINLLDPDAIVLGGGMSNVEAIYPRLPALLSHYVVGRECRTPVVQNLYGCSSGVRGA 300

Query: 293 AWLWEK 298
           AWLWEK
Sbjct: 301 AWLWEK 306


Lambda     K      H
   0.320    0.139    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 410
Number of extensions: 12
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 299
Length of database: 306
Length adjustment: 27
Effective length of query: 272
Effective length of database: 279
Effective search space:    75888
Effective search space used:    75888
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory