Aligments for a candidate for bkdC in Shewanella oneidensis MR-1

GapMind for catabolism of small carbon sources

Aligments for a candidate for bkdC in Shewanella oneidensis MR-1

Align Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase (EC 2.3.1.168) (characterized)
to candidate 201485 SO2341 alpha keto acid dehydrogenase complex, E2 component (NCBI ptt file)

Query= reanno::Marino:GFF1672
         (378 letters)



>FitnessBrowser__MR1:201485
          Length = 535

 Score =  378 bits (971), Expect = e-109
 Identities = 209/388 (53%), Positives = 264/388 (68%), Gaps = 11/388 (2%)

Query: 1   MTDKAMVEITAPKAGRVTKLYHQQQAMAKVHAPLFAFIPRDREEPEEARTKPEPAAQLST 60
           MTDKA+V+I A KAG++ KL++++  +AKVHAPL+A        P  +  +    A  +T
Sbjct: 147 MTDKALVQIPAIKAGKIVKLHYRKGQLAKVHAPLYAIEVEGGVIPAVSAHETTNVAVANT 206

Query: 61  ATASPVAAASRQRIPA-------SPAVRRLVREHELNLSDIQGSGKDGRVLKADVLAYIE 113
           AT++  A AS  + PA       SPAVRR+ R  +++LS + GSGK GRV K D+  +  
Sbjct: 207 ATSAACATASVSQEPARQGKALASPAVRRMARALDIDLSRVPGSGKHGRVYKEDISRFQA 266

Query: 114 EGPKQ--AQNQAPADDAQTATTRSARRAPAADQEAR--VEPIRGIKAAMAKSMVKSATTI 169
           +G         A A   Q++ T+SA     A       VEPIRG+KA MAK MV+S +TI
Sbjct: 267 QGSATPVVAPVATASTQQSSVTQSAVPITVASAARADIVEPIRGVKAVMAKLMVESVSTI 326

Query: 170 PHFIYSEDIDVTDLLKLREQLKPEAEARGSRLTLMPFFMKAMALAVQEFPVLNSQLNDDV 229
           PHF Y E+ D+TDL+ LRE +K +  +   +LT+MPFFMKAM+LA+ +FPVLNSQ+N D 
Sbjct: 327 PHFTYCEEFDLTDLVALRESMKAKYSSDEVKLTMMPFFMKAMSLALTQFPVLNSQVNADC 386

Query: 230 TEIHYLPQCNIGMAVDGKAGLTVPNIKGVESLSLLGIADEVARLTEAARSGRVSQEDLKG 289
           TEI Y  + NIGMAVD K GL VPN+K V+  S+L +A E+ RLT AARSGRV+  DLK 
Sbjct: 387 TEITYKARHNIGMAVDSKVGLLVPNVKDVQDKSILEVAAEITRLTNAARSGRVAPADLKE 446

Query: 290 GTITISNIGALGGTYTAPIINAPEVAIVALGRTQKLPRFDANGQVVERAIMTVSWAGDHR 349
           GTI+ISNIGALGGT   PIIN PEVAIVALG+ Q LPRF+A G+V  R IM VSW+GDHR
Sbjct: 447 GTISISNIGALGGTVATPIINKPEVAIVALGKLQTLPRFNAKGEVEARQIMQVSWSGDHR 506

Query: 350 IIDGGTIARFCNRWKGYLESPQTMLLHM 377
           +IDGGTIARFCN WK YLE PQ MLL M
Sbjct: 507 VIDGGTIARFCNLWKQYLEQPQDMLLAM 534



 Score = 53.5 bits (127), Expect = 1e-11
 Identities = 32/70 (45%), Positives = 46/70 (65%), Gaps = 2/70 (2%)

Query: 1   MTDKAMVEITAPKAGRVTKLYHQQQAMAKVHAPLFAFIPRDREEPEEARTKPEPAAQLST 60
           MTDKA+V+I AP AG VTKLY+ +  +AKVHAPL+A +  + EEP  ++  P+     + 
Sbjct: 40  MTDKALVQIPAPFAGVVTKLYYAKGDIAKVHAPLYA-VQIEAEEP-SSQVAPQTVEHSAP 97

Query: 61  ATASPVAAAS 70
             A+  AA+S
Sbjct: 98  NQAAISAASS 107


Lambda     K      H
   0.316    0.131    0.367 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 476
Number of extensions: 20
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 378
Length of database: 535
Length adjustment: 33
Effective length of query: 345
Effective length of database: 502
Effective search space:   173190
Effective search space used:   173190
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources