GapMind for catabolism of small carbon sources

 

Aligments for a candidate for aldA in Shewanella oneidensis MR-1

Align lactaldehyde dehydrogenase (EC 1.2.1.22); D-glyceraldehyde dehydrogenase (NADP+) (EC 1.2.1.89) (characterized)
to candidate 203555 SO4480 aldehyde dehydrogenase (NCBI ptt file)

Query= BRENDA::P25553
         (479 letters)



>lcl|FitnessBrowser__MR1:203555 SO4480 aldehyde dehydrogenase (NCBI
           ptt file)
          Length = 506

 Score =  246 bits (629), Expect = 1e-69
 Identities = 152/477 (31%), Positives = 253/477 (53%), Gaps = 19/477 (3%)

Query: 10  YIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAEDARKAIDAAERAQPEWEALPAIERA 69
           +I G++V      + D  +P       +IP   A+D   A+DAA  A+  W      ER+
Sbjct: 22  FIGGKWVAPVNGKYFDNRSPVNGQNFCKIPRSDAQDIELALDAAHAAKDAWGKTSVTERS 81

Query: 70  SWLRKISAGIRERASEISALIVEEGGK-IQQLAEVEVAFTADYIDYMAEWARRYEGEIIQ 128
           + L +I+  + +    ++     E GK +++    ++    D+  Y A   R  EG    
Sbjct: 82  NILLRIADRVEQNLEYLAVAETWENGKAVRETLNADLPLFVDHFRYFAGCIRAQEGSAAD 141

Query: 129 SDRPGENILL-FKRALGVTTGILPWNFPFFLIARKMAPALLTGNTIVIKPSEFTPNNAIA 187
            D  G  +   F   LGV   I+PWNFP  + A K+APAL  GN +V+KP+E TP + + 
Sbjct: 142 ID--GNTVSYHFPEPLGVVGQIIPWNFPLLMAAWKIAPALAAGNCVVLKPAEQTPVSILV 199

Query: 188 FAKIVDEIGLPRGVFNLVLGRGETVGQELAGNPKVAMVSMTGSVSAGEKIMATAAKNITK 247
             ++++++ LP GV N+V G G   GQ LA + ++A ++ TGS   G  I+  AA+++  
Sbjct: 200 LLELIEDL-LPPGVLNVVNGFGAEAGQALATSKRIAKLAFTGSTEVGFHILKCAAESLIP 258

Query: 248 VCLELGGKAP----AIVMDDAD--LELAVKAIVDSRVINSGQVCNCAERVYVQKGIYDQF 301
             +ELGGK+P    A VMD  D  L+ AV+ ++ +   N G+VC C  RV +Q+ IYD+F
Sbjct: 259 STVELGGKSPNLYFADVMDQEDEYLDKAVEGMLLA-FFNQGEVCTCPSRVLIQESIYDRF 317

Query: 302 VNRLGEAMQAVQFGNPAERNDIAMGPLINAAALERVEQKVARAVEEGARVAFGG-----K 356
           + ++    Q ++ GNP +     +G   +    +++   +A   +EGA+V  GG     +
Sbjct: 318 IEKVLARAQTIKQGNPLD-TATQVGAQASQEQFDKILSYLAIGKDEGAQVLLGGSLCQLE 376

Query: 357 AVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVVAFDTLEDAISMANDSDYGLTSSIY 416
             + KGYY  PT++     +M I  EE FGPV+ V  F    +A+++AND++YGL + ++
Sbjct: 377 GEQSKGYYISPTIMKG-HNKMRIFQEEIFGPVISVTTFKDEAEALAIANDTEYGLGAGVW 435

Query: 417 TQNLNVAMKAIKGLKFGETYINRENFEAMQGFHAGWRKSGIGGADGKHGLHEYLQTQ 473
           T+++N A +  +G++ G  +IN  +         G++KSGIG    K  L+ Y  T+
Sbjct: 436 TRDMNRAQRMGRGIQAGRVWINCYHAYPAHAAFGGYKKSGIGRETHKMMLNHYQNTK 492


Lambda     K      H
   0.318    0.135    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 578
Number of extensions: 28
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 479
Length of database: 506
Length adjustment: 34
Effective length of query: 445
Effective length of database: 472
Effective search space:   210040
Effective search space used:   210040
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory