GapMind for catabolism of small carbon sources

 

Aligments for a candidate for gtsD in Shewanella oneidensis MR-1

Align ABC transporter for D-Glucose-6-Phosphate, ATPase component (characterized)
to candidate 200449 SO1271 polyamine ABC transporter, ATP-binding protein (NCBI ptt file)

Query= reanno::WCS417:GFF4321
         (386 letters)



>lcl|FitnessBrowser__MR1:200449 SO1271 polyamine ABC transporter,
           ATP-binding protein (NCBI ptt file)
          Length = 378

 Score =  218 bits (555), Expect = 2e-61
 Identities = 116/272 (42%), Positives = 174/272 (63%), Gaps = 2/272 (0%)

Query: 23  NIELSIKEGEFLILVGPSGCGKSTLMNCIAGLETITGGAIMIGDQDVSGMSPKDRDIAMV 82
           ++ L+I +GE   L+G SG GKSTL+  +AG E  T G I +  +D++ + P +R I M+
Sbjct: 38  DVSLTINKGEIFALLGGSGSGKSTLLRMLAGFERPTSGRIYLDGEDITDLPPYERPINMM 97

Query: 83  FQSYALYPTMSVRENIEFGLKIRKMPQADIDAEVARVAKLLQIEHLLNRKPGQLSGGQQQ 142
           FQSYAL+P M+V +NI FGLK  K+P+A+I+  V  + KL+ +E    RKP QLSGGQ+Q
Sbjct: 98  FQSYALFPHMTVAQNIAFGLKQDKLPKAEIEQRVQEMLKLVHMEQYGKRKPHQLSGGQRQ 157

Query: 143 RVAMGRALARRPKIYLFDEPLSNLDAKLRVEMRTEMKLMHQRLKTTTVYVTHDQIEAMTL 202
           RVA+ R+LA+RPK+ L DEP+  LD KLR +M+ E+  + +R+  T V VTHDQ EAMT+
Sbjct: 158 RVALARSLAKRPKLLLLDEPMGALDKKLRTQMQLEVVEILERVGVTCVMVTHDQEEAMTM 217

Query: 203 GDKVAVMKDGIIQQFGTPKEIYNNPANQFVASFIGSPPMNFVPLRLQRKDGRLVALLDSG 262
             ++++M DG I Q G+P +IY +P ++ +A FIGS  +    + +   D  ++   D  
Sbjct: 218 AGRISIMSDGWIAQTGSPMDIYESPNSRMIAEFIGSVNLFSGEIEVDEVDHLIIKPND-- 275

Query: 263 QARCELALNTTEAGLEDRDVILGLRPEQIMLA 294
            AR           LED+ V L +RPE+ +++
Sbjct: 276 LARSFYVGYGVTTSLEDKRVWLAVRPEKTIIS 307


Lambda     K      H
   0.318    0.135    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 310
Number of extensions: 9
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 386
Length of database: 378
Length adjustment: 30
Effective length of query: 356
Effective length of database: 348
Effective search space:   123888
Effective search space used:   123888
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory