Potential Gaps in catabolism of small carbon sources in Marinobacter adhaerens HP15
Found 98 low-confidence and 42 medium-confidence steps on the best paths for 62 pathways.
| Pathway | Step | Best candidate | 2nd candidate |
|---|
| 4-hydroxybenzoate | pcaK: 4-hydroxybenzoate transporter pcaK | | |
| 4-hydroxybenzoate | pobA: 4-hydroxybenzoate 3-monooxygenase | | |
| 4-hydroxybenzoate | praA: protocatechuate 2,3-dioxygenase | | |
| acetate | actP: cation/acetate symporter ActP | | |
| arabinose | araA: L-arabinose isomerase | | |
| arabinose | araB: ribulokinase | | |
| arabinose | araD: L-ribulose-5-phosphate epimerase | | |
| arabinose | araE: L-arabinose:H+ symporter | | |
| asparagine | ans: asparaginase | HP15_1914 | |
| cellobiose | bgl: cellobiase | | |
| citrulline | AO353_03040: ABC transporter for L-Citrulline, ATPase component | HP15_3030 | HP15_2920 |
| citrulline | AO353_03050: ABC transporter for L-Citrulline, permease component 1 | HP15_3032 | HP15_2919 |
| citrulline | AO353_03055: ABC transporter for L-Citrulline, periplasmic substrate-binding component | HP15_3031 | |
| citrulline | gabT: gamma-aminobutyrate transaminase | HP15_3708 | HP15_2925 |
| citrulline | odc: L-ornithine decarboxylase | HP15_3785 | |
| citrulline | puuD: gamma-glutamyl-gamma-aminobutyrate hydrolase | HP15_2338 | |
| D-alanine | Pf6N2E2_5402: ABC transporter for D-Alanine, substrate-binding component | HP15_2196 | |
| D-lactate | lctP: D-lactate:H+ symporter LctP or LidP | | |
| D-serine | cycA: D-serine:H+ symporter CycA | | |
| D-serine | dsdA: D-serine ammonia-lyase | HP15_3213 | |
| deoxyinosine | deoB: phosphopentomutase | HP15_3072 | |
| deoxyinosine | deoC: deoxyribose-5-phosphate aldolase | | |
| deoxyinosine | deoD: deoxyinosine phosphorylase | HP15_2259 | |
| deoxyinosine | nupC: deoxyinosine:H+ symporter NupC | | |
| deoxyribonate | deoxyribonate-dehyd: 2-deoxy-D-ribonate 3-dehydrogenase | HP15_825 | HP15_1707 |
| deoxyribonate | deoxyribonate-transport: 2-deoxy-D-ribonate transporter | | |
| deoxyribonate | garK: glycerate 2-kinase | | |
| deoxyribonate | ketodeoxyribonate-cleavage: 2-deoxy-3-keto-D-ribonate cleavage enzyme | | |
| deoxyribose | deoC: deoxyribose-5-phosphate aldolase | | |
| deoxyribose | deoK: deoxyribokinase | | |
| deoxyribose | deoP: deoxyribose transporter | | |
| fucose | aldA: lactaldehyde dehydrogenase | HP15_943 | HP15_3144 |
| fucose | fucA: L-fuculose-phosphate aldolase FucA | | |
| fucose | fucI: L-fucose isomerase FucI | | |
| fucose | fucK: L-fuculose kinase FucK | | |
| fucose | fucP: L-fucose:H+ symporter FucP | | |
| fucose | fucU: L-fucose mutarotase FucU | | |
| galactose | galE: UDP-glucose 4-epimerase | HP15_2048 | HP15_p187g66 |
| galactose | galK: galactokinase (-1-phosphate forming) | | |
| galactose | galT: UDP-glucose:alpha-D-galactose-1-phosphate uridylyltransferase | | |
| galacturonate | exuT: D-galacturonate transporter ExuT | | |
| galacturonate | kdgK: 2-keto-3-deoxygluconate kinase | | |
| galacturonate | uxaA: D-altronate dehydratase | | |
| galacturonate | uxaB: tagaturonate reductase | | |
| galacturonate | uxaC: D-galacturonate isomerase | | |
| gluconate | gntK: D-gluconate kinase | | |
| gluconate | gntT: gluconate:H+ symporter GntT | | |
| glucosamine | nagB: glucosamine 6-phosphate deaminase (isomerizing) | HP15_3676 | HP15_p187g69 |
| glucosamine | SLC2A2: glucosamine transporter SLC2A2 | | |
| glucose-6-P | uhpT: glucose-6-phosphate:phosphate antiporter | | |
| glucuronate | dopDH: 2,5-dioxopentanonate dehydrogenase | HP15_2020 | HP15_3626 |
| glucuronate | exuT: D-glucuronate:H+ symporter ExuT | | |
| glucuronate | gci: D-glucaro-1,4-lactone cycloisomerase | | |
| glucuronate | kdgD: 5-dehydro-4-deoxyglucarate dehydratase | HP15_2027 | |
| glucuronate | udh: D-glucuronate dehydrogenase | | |
| histidine | hutG: N-formiminoglutamate formiminohydrolase | | |
| histidine | hutH: histidine ammonia-lyase | | |
| histidine | hutI: imidazole-5-propionate hydrolase | | |
| histidine | hutU: urocanase | | |
| L-lactate | Shew_2731: L-lactate:Na+ symporter, large component | HP15_1251 | |
| L-lactate | Shew_2732: L-lactate:Na+ symporter, small component | HP15_1252 | |
| lactose | klh: periplasmic 3'-ketolactose hydrolase | | |
| lactose | lacA': periplasmic lactose 3-dehydrogenase, LacA subunit | | |
| lactose | lacB': periplasmic lactose 3-dehydrogenase, cytochrome c component (LacB) | | |
| lactose | lacC': periplasmic lactose 3-dehydrogenase, LacC subunit | | |
| lysine | argT: L-lysine ABC transporter, substrate-binding component ArgT | HP15_3031 | HP15_2918 |
| lysine | davA: 5-aminovaleramidase | HP15_2074 | HP15_3653 |
| lysine | davB: L-lysine 2-monooxygenase | | |
| lysine | gcdG: succinyl-CoA:glutarate CoA-transferase | HP15_1416 | HP15_2609 |
| lysine | hisM: L-lysine ABC transporter, permease component 1 (HisM) | HP15_3033 | HP15_3032 |
| lysine | hisP: L-lysine ABC transporter, ATPase component HisP | HP15_2193 | HP15_3030 |
| lysine | hisQ: L-lysine ABC transporter, permease component 2 (HisQ) | HP15_3032 | HP15_2919 |
| maltose | susB: alpha-glucosidase (maltase) | | |
| mannitol | mtlA: mannitol phosphotransferase system, EII-CBA components | | |
| mannitol | mtlD: mannitol-1-phosphate 5-dehydrogenase | | |
| mannose | manA: mannose-6-phosphate isomerase | HP15_444 | HP15_3072 |
| mannose | manP: mannose PTS system, EII-CBA components | HP15_3725 | |
| myoinositol | iolB: 5-deoxy-D-glucuronate isomerase | | |
| myoinositol | iolC: 5-dehydro-2-deoxy-D-gluconate kinase | | |
| myoinositol | iolD: 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase | | |
| myoinositol | iolE: scyllo-inosose 2-dehydratase | | |
| myoinositol | iolG: myo-inositol 2-dehydrogenase | | |
| myoinositol | iolJ: 5-dehydro-2-deoxyphosphogluconate aldolase | HP15_2910 | |
| myoinositol | iolT: myo-inositol:H+ symporter | | |
| NAG | nagA: N-acetylglucosamine 6-phosphate deacetylase | | |
| NAG | nagB: glucosamine 6-phosphate deaminase (isomerizing) | HP15_3676 | HP15_p187g69 |
| NAG | nagEcba: N-acetylglucosamine phosphotransferase system, EII-CBA components | | |
| phenylacetate | paaF: 2,3-dehydroadipyl-CoA hydratase | HP15_12 | HP15_908 |
| phenylacetate | paaT: phenylacetate transporter Paa | | |
| phenylalanine | paaF: 2,3-dehydroadipyl-CoA hydratase | HP15_12 | HP15_908 |
| propionate | putP: propionate transporter; proline:Na+ symporter | HP15_2205 | |
| putrescine | gabT: gamma-aminobutyrate transaminase | HP15_3708 | HP15_2925 |
| putrescine | puuD: gamma-glutamyl-gamma-aminobutyrate hydrolase | HP15_2338 | |
| rhamnose | aldA: lactaldehyde dehydrogenase | HP15_943 | HP15_3144 |
| rhamnose | LRA1: L-rhamnofuranose dehydrogenase | HP15_42 | HP15_2784 |
| rhamnose | LRA2: L-rhamnono-gamma-lactonase | | |
| rhamnose | LRA3: L-rhamnonate dehydratase | | |
| rhamnose | LRA4: 2-keto-3-deoxy-L-rhamnonate aldolase | | |
| rhamnose | rhaT: L-rhamnose:H+ symporter RhaT | | |
| ribose | rbsK: ribokinase | | |
| ribose | rbsU: probable D-ribose transporter RbsU | | |
| serine | braD: L-alanine/L-serine/L-threonine ABC transporter, permease component 1 (BraD/NatD) | HP15_3056 | HP15_2706 |
| serine | braF: L-alanine/L-serine/L-threonine ABC transporter, ATP-binding component 1 (BraF/NatA) | HP15_3058 | HP15_2704 |
| serine | sdaB: L-serine ammonia-lyase | HP15_3213 | |
| sorbitol | mtlA: PTS system for polyols, EII-CBA components | | |
| sorbitol | srlD: sorbitol 6-phosphate 2-dehydrogenase | HP15_2361 | HP15_2784 |
| sucrose | ams: sucrose hydrolase (invertase) | | |
| threonine | braD: L-alanine/L-serine/L-threonine ABC transporter, permease component 1 (BraD/NatD) | HP15_3056 | HP15_2706 |
| threonine | braF: L-alanine/L-serine/L-threonine ABC transporter, ATP-binding component 1 (BraF/NatA) | HP15_3058 | HP15_2704 |
| threonine | tdcE: 2-ketobutyrate formate-lyase | | |
| thymidine | deoA: thymidine phosphorylase DeoA | HP15_2594 | |
| thymidine | deoB: phosphopentomutase | HP15_3072 | |
| thymidine | deoC: deoxyribose-5-phosphate aldolase | | |
| thymidine | nupG: thymidine permease NupG/XapB | | |
| trehalose | treF: trehalase | | |
| tryptophan | antA: anthranilate 1,2-dioxygenase (deaminating, decarboxylating), large subunit AntA | HP15_1106 | HP15_39 |
| tryptophan | antB: anthranilate 1,2-dioxygenase (deaminating, decarboxylating), small subunit AntB | | |
| tryptophan | antC: anthranilate 1,2-dioxygenase (deaminating, decarboxylating), electron transfer component AntC | HP15_1107 | HP15_4029 |
| tryptophan | aroP: tryptophan:H+ symporter AroP | | |
| tryptophan | kyn: kynureninase | | |
| tryptophan | kynA: tryptophan 2,3-dioxygenase | | |
| tryptophan | kynB: kynurenine formamidase | | |
| tyrosine | Ac3H11_1692: L-tyrosine ABC transporter, ATPase component 2 | HP15_2703 | HP15_3059 |
| tyrosine | Ac3H11_1694: L-tyrosine ABC transporter, permease component 2 | HP15_3057 | HP15_2705 |
| tyrosine | Ac3H11_1695: L-tyrosine ABC transporter, permease component 1 | HP15_3056 | HP15_2706 |
| tyrosine | Ac3H11_2396: L-tyrosine ABC transporter, substrate-binding component component | HP15_3055 | |
| tyrosine | fahA: fumarylacetoacetate hydrolase | | |
| tyrosine | hmgA: homogentisate dioxygenase | | |
| tyrosine | HPD: 4-hydroxyphenylpyruvate dioxygenase | | |
| tyrosine | maiA: maleylacetoacetate isomerase | | |
| valine | acdH: isobutyryl-CoA dehydrogenase | HP15_907 | HP15_2 |
| valine | bch: 3-hydroxyisobutyryl-CoA hydrolase | HP15_3410 | HP15_909 |
| xylitol | fruI: xylitol PTS, enzyme IIABC (FruI) | HP15_3725 | |
| xylitol | x5p-reductase: D-xylulose-5-phosphate 2-reductase | | |
| xylose | gtsA: xylose ABC transporter, periplasmic substrate-binding component GtsA | HP15_2958 | |
| xylose | gtsB: xylose ABC transporter, permease component 1 GtsB | HP15_2957 | |
| xylose | gtsC: xylose ABC transporter, permease component 2 GtsC | HP15_2956 | |
| xylose | gtsD: xylose ABC transporter, ATPase component GtsD | HP15_2955 | HP15_939 |
| xylose | xylA: xylose isomerase | | |
| xylose | xylB: xylulokinase | | |
Confidence: high confidence medium confidence low confidence
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory