GapMind for catabolism of small carbon sources

 

Protein GFF2245 in Marinobacter adhaerens HP15

Annotation: FitnessBrowser__Marino:GFF2245

Length: 395 amino acids

Source: Marino in FitnessBrowser

Candidate for 17 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
D-alanine catabolism Pf6N2E2_5403 hi ABC transporter for D-Alanine, permease component 2 (characterized) 58% 100% 433.7 ABC transporter for L-Glutamine, L-Histidine, and other L-amino acids, permease component 1 51% 384.0
L-histidine catabolism aapQ med ABC transporter for L-Glutamine, L-Histidine, and other L-amino acids, permease component 1 (characterized) 51% 95% 384 ABC transporter for D-Alanine, permease component 2 58% 433.7
L-asparagine catabolism aapQ med AapQ, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 51% 100% 382.9 ABC transporter for D-Alanine, permease component 2 58% 433.7
L-aspartate catabolism aapQ med AapQ, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 51% 100% 382.9 ABC transporter for D-Alanine, permease component 2 58% 433.7
L-glutamate catabolism aapQ med AapQ, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 51% 100% 382.9 ABC transporter for D-Alanine, permease component 2 58% 433.7
L-leucine catabolism aapQ med AapQ, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 51% 100% 382.9 ABC transporter for D-Alanine, permease component 2 58% 433.7
L-proline catabolism aapQ med AapQ, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 51% 100% 382.9 ABC transporter for D-Alanine, permease component 2 58% 433.7
L-asparagine catabolism bztB med glutamate/glutamine/aspartate/asparagine transport system permease protein BztB (characterized) 43% 96% 292.7 ABC transporter for D-Alanine, permease component 2 58% 433.7
L-aspartate catabolism bztB med glutamate/glutamine/aspartate/asparagine transport system permease protein BztB (characterized) 43% 96% 292.7 ABC transporter for D-Alanine, permease component 2 58% 433.7
L-glutamate catabolism bztB med glutamate/glutamine/aspartate/asparagine transport system permease protein BztB (characterized) 43% 96% 292.7 ABC transporter for D-Alanine, permease component 2 58% 433.7
L-asparagine catabolism bgtB' med ABC-type permease for basic amino acids and glutamine (characterized, see rationale) 42% 95% 260.4 ABC transporter for D-Alanine, permease component 2 58% 433.7
L-aspartate catabolism bgtB' med ABC-type permease for basic amino acids and glutamine (characterized, see rationale) 42% 95% 260.4 ABC transporter for D-Alanine, permease component 2 58% 433.7
L-asparagine catabolism aatQ lo Glutamate/aspartate import permease protein GltJ (characterized) 36% 61% 88.2 ABC transporter for D-Alanine, permease component 2 58% 433.7
L-aspartate catabolism aatQ lo Glutamate/aspartate import permease protein GltJ (characterized) 36% 61% 88.2 ABC transporter for D-Alanine, permease component 2 58% 433.7
L-glutamate catabolism gltJ lo Glutamate/aspartate import permease protein GltJ (characterized) 36% 61% 88.2 ABC transporter for D-Alanine, permease component 2 58% 433.7
L-asparagine catabolism peb1D lo Amino acid ABC transporter, permease protein PEB1 (characterized, see rationale) 31% 60% 73.6 ABC transporter for D-Alanine, permease component 2 58% 433.7
L-aspartate catabolism peb1D lo Amino acid ABC transporter, permease protein PEB1 (characterized, see rationale) 31% 60% 73.6 ABC transporter for D-Alanine, permease component 2 58% 433.7

Sequence Analysis Tools

View GFF2245 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MKKQTIDTRPAGPKPWYDPRVRSLFFQAVAIALVFWGGWILVDNTLSNMESRGISTGFGF
LGETAGFGIIMNLVPYDATMSYGRTFWVGLTNTLLVSAMGVVAATILGFIIGVARLSSNW
LVAKMALVYIEVIRNIPLLLQIFFWYFAVLSNLPSPRQSVDVGGALFLNNRGLYLPDPVT
QEGFGIVWGGILLAIAAVVGIRIWAKKRQLATGQIFPTFKVGVAILVLVPIISYLVAGRP
LEWDLPALRGFNFGGGITIIPELAALWIALSLYTASFIAEIVRSGILSVSKGQTEASKAL
GLPNGLTLRLVVIPQAMRVIIPPLTSQYLNLVKNSSLATAIGYPDLVAVFMGTTLNQTGQ
AVEVVAITMAVYLTISLLISLFMNIYNRAVAIKER

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory