Align 2-oxopent-4-enoate hydratase subunit (EC 4.2.1.80) (characterized)
to candidate GFF1135 HP15_1113 hydratase/decarboxylase
Query= metacyc::MONOMER-3403 (222 letters) >FitnessBrowser__Marino:GFF1135 Length = 261 Score = 370 bits (950), Expect = e-107 Identities = 182/221 (82%), Positives = 199/221 (90%) Query: 1 MDKTLINELGDELYQAMVQRETVTPLTSRGFDISVEDAYHISLRMLERRLAAGERVIGKK 60 M+++ I LGDELYQAM+ RE V PLT RG DIS++DAYHISLRMLERRLA G +IGKK Sbjct: 1 MEQSKIQALGDELYQAMLSREAVEPLTGRGEDISIDDAYHISLRMLERRLADGASIIGKK 60 Query: 61 IGVTSKAVQNMLGVHQPDFGYLTDAMVYNSGEAMPISEKLIQPRAEGEIAFILKKDLMGP 120 IGVTSKAVQNML VHQPDFGYLTD MV+NSGE + IS++LI PRAEGEIAFILKKDL GP Sbjct: 61 IGVTSKAVQNMLNVHQPDFGYLTDDMVFNSGETVRISDRLIAPRAEGEIAFILKKDLSGP 120 Query: 121 GVTNADVLAATECVIPCFEVVDSRIQDWKIKIQDTVADNASCGLFVLGDQAVSPRQVDLV 180 GVTNADVLAATECV+PCFE+VDSRIQDWKI IQDT+ADNASCGLFVLGDQAVSPR+VDLV Sbjct: 121 GVTNADVLAATECVMPCFEIVDSRIQDWKIAIQDTIADNASCGLFVLGDQAVSPRKVDLV 180 Query: 181 TCGMLVEKNGQLLSTGAGAAALGSPVNCVAWLANTLGHFGI 221 TCGM+VEKNG +LS GAGAAALGSPVNCV WLANTLG FGI Sbjct: 181 TCGMVVEKNGSVLSAGAGAAALGSPVNCVTWLANTLGEFGI 221 Lambda K H 0.319 0.136 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 239 Number of extensions: 7 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 222 Length of database: 261 Length adjustment: 23 Effective length of query: 199 Effective length of database: 238 Effective search space: 47362 Effective search space used: 47362 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory