Align High-affinity branched-chain amino acid transport ATP-binding protein BraF, component of Branched chain amino acid uptake transporter. Transports alanine (characterized)
to candidate GFF947 HP15_926 branched-chain amino acid ABC transporter, ATP-binding protein
Query= TCDB::P21629 (255 letters) >FitnessBrowser__Marino:GFF947 Length = 267 Score = 167 bits (424), Expect = 2e-46 Identities = 97/250 (38%), Positives = 147/250 (58%), Gaps = 9/250 (3%) Query: 5 ILEVSGLTMRFGGLLAVNGVNLKVEEKQVVSMIGPNGAGKTTVFNCLTGFYQPTGGLIRL 64 +LE L F G +AV+ VNLKV++ + ++IGPNGAGKTTVFN LT F PT G I Sbjct: 6 VLETRNLVKEFKGFVAVDDVNLKVQKGHIHALIGPNGAGKTTVFNLLTKFLIPTRGQILF 65 Query: 65 DGEEIQGLPGHKIARKGVVRTFQNVRLFKEMTAVENLLVAQHRHLNTNFLAGLFKT-PAF 123 ++I + IAR+G+VR+FQ +F MTA+EN+ VA T+F +K+ + Sbjct: 66 KDQDITTMKSAAIARQGIVRSFQISAVFPHMTALENIRVALQTFEGTSF--SFWKSGNSL 123 Query: 124 RRSEREAMEYAAHWLEEVNLTEFANRSAGTLAYGQQRRLEIARCMMTRPRILMLDEPAAG 183 + + ME L+ V L EFAN + LAYG++R LE+A + P++L+LDEP G Sbjct: 124 HKLNQRCMEL----LDAVGLAEFANTTTVELAYGRKRALELATTLAMEPQLLLLDEPTQG 179 Query: 184 LNPKETDDLKALIAKLRSEHNVTVLLIEHDMKLVMSISDHIVVINQGAPLADGTPEQIRD 243 + ++ D + L+ K + TVL++EH++ +V + D I V+ QGA L +G + Sbjct: 180 MGSEDVDRVVELVRK--AAEGRTVLMVEHNLSVVSKLCDRITVLAQGAVLTEGDYATVSG 237 Query: 244 NPDVIKAYLG 253 +P V + Y+G Sbjct: 238 DPRVREVYMG 247 Lambda K H 0.320 0.137 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 139 Number of extensions: 5 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 255 Length of database: 267 Length adjustment: 25 Effective length of query: 230 Effective length of database: 242 Effective search space: 55660 Effective search space used: 55660 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory