Align Probable 2-ketoarginine decarboxylase AruI; 2-oxo-5-guanidinopentanoate decarboxylase; 5-guanidino-2-oxopentanoate decarboxylase; EC 4.1.1.75 (characterized)
to candidate GFF1038 HP15_1017 acetolactate synthase, large subunit, biosynthetic type
Query= SwissProt::Q9HUI8 (559 letters) >FitnessBrowser__Marino:GFF1038 Length = 566 Score = 204 bits (519), Expect = 7e-57 Identities = 163/537 (30%), Positives = 243/537 (45%), Gaps = 21/537 (3%) Query: 30 LTAGQALVRLLANYGVDTVFGIPGVHTLELYRGLPGS-GIRHVLTRHEQGAGFMADGYAR 88 + Q ++ + + TVFG PG + LY L G+ HVL RHEQG ADGYAR Sbjct: 1 MNGAQHILEAFHRHNISTVFGYPGGCIMPLYDALVDDVGVEHVLCRHEQGCALAADGYAR 60 Query: 89 VSGKPGVCFVITGPGVTNVATAIGQAYADSVPLLVISSVNHSASLGKGWGCLHETQDQRA 148 SGK GVC +GPG TN+ T + A+ DS+P+LVI+ S +G ET D Sbjct: 61 ASGKLGVCIATSGPGATNLITGVANAHRDSIPMLVITGQVPSGLIGT--DAFQET-DVLG 117 Query: 149 MTAPITAFSALALSPEQLPELIARAYAVFDSERPRPVHISIPLDVLAAPVAHDWSAAVAR 208 MT I S L + LP ++ A + S RP PV I IP D+L + VA A Sbjct: 118 MTLGIVKHSYLINDADSLPIIMEEAIELAQSGRPGPVWIDIPKDLLLSDVAD----APFP 173 Query: 209 RPGRGVPCSEALRAAAERLAAARRPMLIAGGGALA--AGEALAALSERLAAPLFTSVAGK 266 + P S L+ A L AAR+P+L +GGG A E A +E A P ++ G Sbjct: 174 QAQPYEPASPDLKEALAMLRAARKPLLYSGGGISLGHAEENFRAFAESSALPAVVTLKGI 233 Query: 267 GLLPPDAPLNAG--ASLCVAPGWEMIAEADLVLAVGTEMADTDFWR-ERLPLSGELIRVD 323 G P N G + + E DL+L +G + D + + + +I +D Sbjct: 234 GNPGKHNPYNLGMLGMHGSRAANKAVDECDLLLVIGARLDDRATGKLDGFAPNARMIHID 293 Query: 324 IDPRKFNDFYPSAVALRGDARQTLEALLVRLPQE--ARDSAPAAARVARLRAEIRAA--H 379 D + N + +A+RGD L++L L + A R AA Sbjct: 294 ADAAEINKLRDADLAIRGDLNHILKSLAGELHADPLAISEWQKQCRSWYTTGGFHAADNE 353 Query: 380 APLQAL-HQAILDRIAAALPADAFVSTDMTQLAYTGNYAFASRAPRSWLHPTGYGTLGYG 438 PL + A + +++ P D ++ D+ Q + PR L G GT+G+G Sbjct: 354 EPLAPITGPAFIRQLSRIAPDDTVIACDVGQHQMWVAQHYDFDHPRHHLTSGGLGTMGFG 413 Query: 439 LPAGIGAKLGAPQRPGLVLVGDGGFLYTAQELATASEELDSPLVVLLWNNDALGQIR--D 496 LPA IGA+ + + GDG F+ AQELAT + P+ +++ +N LG +R Sbjct: 414 LPAAIGAQFADRNSTVINVTGDGSFMMNAQELATI-RRYNLPVKLIVMDNQCLGMVRQQQ 472 Query: 497 DMLGLDIEPVGVLPRNPDFALLGRAYGCAVRQPQDLDELERDLRAGFGQSGVTLIEL 553 ++ + E L NPDF + RA+ + D++ R + +G L+ + Sbjct: 473 ELFYNNRESQINLDDNPDFVAMARAFDIPALYIERTDQIRRGIETILAYNGPMLLHV 529 Lambda K H 0.321 0.136 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 741 Number of extensions: 38 Number of successful extensions: 7 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 559 Length of database: 566 Length adjustment: 36 Effective length of query: 523 Effective length of database: 530 Effective search space: 277190 Effective search space used: 277190 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory