Align Succinylarginine dihydrolase (EC 3.5.3.23) (characterized)
to candidate GFF3095 HP15_3038 N-succinylarginine dihydrolase
Query= reanno::SB2B:6937151 (444 letters) >FitnessBrowser__Marino:GFF3095 Length = 446 Score = 540 bits (1391), Expect = e-158 Identities = 280/445 (62%), Positives = 326/445 (73%), Gaps = 3/445 (0%) Query: 1 MKH-FEANFDGLVGPTHNYAGLSFGNVASQSNAAQVSNPKDAAKQGLKKAKALADMGMVQ 59 +KH EANFDGLVGPTHNYAGLS+GNVAS+SN + VSNPK+AA QGL K K LAD G VQ Sbjct: 2 VKHAVEANFDGLVGPTHNYAGLSWGNVASKSNVSSVSNPKEAALQGLAKMKRLADRGYVQ 61 Query: 60 GMLAPQERPDIHTLRRVGFTGSDADVLSQAAKASPVLLQACASASSMWTANAATVSPSAD 119 G+L P ERP I TLR +GF G DA VL QAAK+SP +L A +SAS MWTANAATVSPSAD Sbjct: 62 GVLPPHERPHIPTLRALGFEGPDARVLEQAAKSSPSILAAVSSASPMWTANAATVSPSAD 121 Query: 120 SDDGKLHFTPANLVDKLHRSIEPVTTGNILKAIFTDERYFAHHQHLPEHPHFGDEGAANH 179 + D ++HFTPANL K HRSIE TG LK+IF DE YFAHH LP HFGDEGAANH Sbjct: 122 TSDHRVHFTPANLSAKFHRSIEHAVTGRALKSIFADESYFAHHPALPSVSHFGDEGAANH 181 Query: 180 TRLCHDYGQAGVEVFVYGRSVADLSRPAPVKYPARQTLEASQAVARLHQLSDDRTVYMQQ 239 TRLC YG+ GVE+FVYG+ + PAP KYPARQTLEASQAVARLH L D V+ QQ Sbjct: 182 TRLCAGYGEPGVELFVYGQMAFNEQAPAPKKYPARQTLEASQAVARLHGLRDQNAVFAQQ 241 Query: 240 NPDVIDQGVFHNDVIAVGNQNVLFYHEQAFLETQAKLAEIDKKMHG-NMYFIEVPTAKVS 298 NPD ID GVFHNDVIAVGN N LFYHE AFL LA+I +++ G + + V +A V Sbjct: 242 NPDAIDGGVFHNDVIAVGNGNTLFYHEMAFLNEAQVLADIRERLTGAELEAVRVSSADVP 301 Query: 299 VQDAVKSYLFNTQIITLSDGNMAIIAPTDCQENPAVHAYLNELVTLNTPIKAVHYFDVKQ 358 ++DAV SYLFN+Q++ DG M + P +C+E +V YL+ LV PI AV FDVKQ Sbjct: 302 LEDAVASYLFNSQLLNTPDG-MLLAVPGECREVASVSRYLDGLVKSGGPITAVEVFDVKQ 360 Query: 359 SMQNGGGPACLRLRVAMNETELAAVNPQVMMNDALFARLNQWVDKHYRDRLSTQDLADPQ 418 SM+NGGGPACLRLRV +N+ EL A+N V++ D L+ RL WV+ HYRD LS +L DP Sbjct: 361 SMRNGGGPACLRLRVVLNDDELKAINRGVLLTDELYERLTTWVEAHYRDELSQNELGDPM 420 Query: 419 LLMESRTALDELTQIMKLGSVYQFQ 443 LL E R ALDELT IM LGS+Y FQ Sbjct: 421 LLEEVRKALDELTGIMGLGSIYDFQ 445 Lambda K H 0.318 0.131 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 552 Number of extensions: 18 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 444 Length of database: 446 Length adjustment: 32 Effective length of query: 412 Effective length of database: 414 Effective search space: 170568 Effective search space used: 170568 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
Align candidate GFF3095 HP15_3038 (N-succinylarginine dihydrolase)
to HMM TIGR03241 (astB: succinylarginine dihydrolase (EC 3.5.3.23))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR03241.hmm # target sequence database: /tmp/gapView.17383.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR03241 [M=443] Accession: TIGR03241 Description: arg_catab_astB: succinylarginine dihydrolase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 7.6e-226 736.2 0.0 8.6e-226 736.0 0.0 1.0 1 lcl|FitnessBrowser__Marino:GFF3095 HP15_3038 N-succinylarginine dih Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Marino:GFF3095 HP15_3038 N-succinylarginine dihydrolase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 736.0 0.0 8.6e-226 8.6e-226 1 443 [] 5 445 .. 5 445 .. 0.99 Alignments for each domain: == domain 1 score: 736.0 bits; conditional E-value: 8.6e-226 TIGR03241 1 ayevnfdGlvGlthnyaGlsfGnkastsnkksvsnpklaakqGllkmkaladlGfkqgvlapqerpdiaalrklG 75 a e+nfdGlvG+thnyaGls+Gn+as+sn +svsnpk+aa qGl+kmk lad G+ qgvl+p+erp+i++lr lG lcl|FitnessBrowser__Marino:GFF3095 5 AVEANFDGLVGPTHNYAGLSWGNVASKSNVSSVSNPKEAALQGLAKMKRLADRGYVQGVLPPHERPHIPTLRALG 79 579************************************************************************ PP TIGR03241 76 fsGsdeevlekaareapellsavssassmwtanaatvspsadtadgrvhftaanlnnkfhrsieaettervlkai 150 f G d+ vle+aa+++p++l+avssas+mwtanaatvspsadt+d rvhft+anl++kfhrsie+++t r+lk+i lcl|FitnessBrowser__Marino:GFF3095 80 FEGPDARVLEQAAKSSPSILAAVSSASPMWTANAATVSPSADTSDHRVHFTPANLSAKFHRSIEHAVTGRALKSI 154 *************************************************************************** PP TIGR03241 151 fadekkfavhealpavallGdeGaanhtrlgaeydepgvelfvyGraal.erepkpkryparqtleasqavarlh 224 fade++fa+h alp+v+++GdeGaanhtrl+a y+epgvelfvyG+ a+ e++p+pk+yparqtleasqavarlh lcl|FitnessBrowser__Marino:GFF3095 155 FADESYFAHHPALPSVSHFGDEGAANHTRLCAGYGEPGVELFVYGQMAFnEQAPAPKKYPARQTLEASQAVARLH 229 *************************************************8899********************** PP TIGR03241 225 qleeekvvyaqqnpdvidqGvfhndviavsnrevlfhhekaflnqsqvldelraklaalgqelvaievpdaevsv 299 +l+++++v+aqqnpd+id Gvfhndviav+n ++lf+he afln++qvl+++r++l++ el+a++v++a+v + lcl|FitnessBrowser__Marino:GFF3095 230 GLRDQNAVFAQQNPDAIDGGVFHNDVIAVGNGNTLFYHEMAFLNEAQVLADIRERLTGA--ELEAVRVSSADVPL 302 ********************************************************987..9************* PP TIGR03241 300 edavssylfnsqllskedgkmllvvpeecreneavwayldelvaadgpikevkvfdlresmknGGGpaclrlrvv 374 edav sylfnsqll+ dg mll vp ecre ++v +yld lv+++gpi+ v+vfd+++sm+nGGGpaclrlrvv lcl|FitnessBrowser__Marino:GFF3095 303 EDAVASYLFNSQLLNTPDG-MLLAVPGECREVASVSRYLDGLVKSGGPITAVEVFDVKQSMRNGGGPACLRLRVV 376 ***************9998.999**************************************************** PP TIGR03241 375 lndaelaavnpkvllsdalfatlnkwvdrhyrdrlsakdladpqllvesrtaldeltqilnlGsvyefq 443 lnd+el+a+n+ vll+d+l+++l++wv+ hyrd+ls+++l dp ll+e+r+aldelt i++lGs+y+fq lcl|FitnessBrowser__Marino:GFF3095 377 LNDDELKAINRGVLLTDELYERLTTWVEAHYRDELSQNELGDPMLLEEVRKALDELTGIMGLGSIYDFQ 445 ********************************************************************9 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (443 nodes) Target sequences: 1 (446 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.00s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 9.23 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory