GapMind for catabolism of small carbon sources

 

Aligments for a candidate for patD in Marinobacter adhaerens HP15

Align 4-(gamma-glutamylamino)butanal dehydrogenase (EC 1.2.1.99) (characterized)
to candidate GFF4092 HP15_4032 betaine aldehyde dehydrogenase

Query= BRENDA::P23883
         (495 letters)



>lcl|FitnessBrowser__Marino:GFF4092 HP15_4032 betaine aldehyde
           dehydrogenase
          Length = 486

 Score =  337 bits (865), Expect = 4e-97
 Identities = 189/483 (39%), Positives = 286/483 (59%), Gaps = 15/483 (3%)

Query: 23  FINGEYTAAAENETFETVDPVTQAPLAKIARGKSVDIDRAMSAARGVFERGDWSLSSPAK 82
           +ING+Y  +A    F+ V+P     ++K+      ++D A+ AAR    RG W   +  +
Sbjct: 7   YINGQYVGSASGRLFDNVNPANGKVISKVHEAGREEVDAAVKAARAAL-RGPWGKMTLEQ 65

Query: 83  RKAVLNKLADLMEAHAEELALLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEV 142
           R A+L+K+AD + A  +E    E LDTGKP   +   DIP  A   + +A+ I  V  E 
Sbjct: 66  RTAILHKVADGINARFDEFLEAECLDTGKPKSLASHIDIPRGAANFKVFADMIKNVPTES 125

Query: 143 ATTSSHE----LAMIVREPVGVIAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPL 198
               + +    L   VR P GVI  I PWN PLLL  WK+GPALA GN+V++KPSE++P 
Sbjct: 126 FEMPTPDGTGALNYAVRRPKGVIGVISPWNLPLLLMTWKVGPALACGNTVVVKPSEETPT 185

Query: 199 SAIRLAGLAKEAGLPDGVLNVVTGFGHE-AGQALSRHNDIDAIAFTGSTRTGKQLLKDAG 257
           +   L  + KEAG+PDGV NVV GFG + AG  L+ H  +D   FTG T TG+ ++K A 
Sbjct: 186 TTALLGEVMKEAGVPDGVYNVVHGFGGDSAGAYLTEHPQVDGFTFTGETGTGEVIMKAAA 245

Query: 258 DSNMKRVWLEAGGKSANIVFADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADE 317
              ++ + LE GGK+A +VFADC D+++A   T    F N GQVC+   R+ +E SI DE
Sbjct: 246 -KGIRDISLELGGKNAGLVFADC-DMEKAIEGTMRSAFANCGQVCLGTERVYVERSIFDE 303

Query: 318 FLALLKQQAQNWQPGHPLDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRN----- 372
           F+  LK+ A+  + G P D    +G L+   H + V S+ ++    G  ++ G       
Sbjct: 304 FVGRLKEAAEGLKIGPPDDADANLGPLVSLKHREKVLSYYQKAVDDGATVVTGGGVPDMP 363

Query: 373 AGLA--AAIGPTIFVDVDPNASLSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVW 430
           A LA  A + PTI+  +  ++++  +EIFGP   +  F +EE+A++LAN   YGL +A+W
Sbjct: 364 AELAGGAWVEPTIWTGLPDDSAVVTDEIFGPCCHIRPFDTEEEAIELANSLPYGLASAIW 423

Query: 431 TRDLSRAHRMSRRLKAGSVFVNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIW 490
           + +++RAHR++ +++AG ++VN++   D+  PFGG KQSG GR+  +H+LE +TE+K I 
Sbjct: 424 SENITRAHRVAGQIEAGIIWVNSWFLRDLRTPFGGSKQSGVGREGGVHSLEFYTEMKNIC 483

Query: 491 ISL 493
           + L
Sbjct: 484 VKL 486


Lambda     K      H
   0.317    0.133    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 612
Number of extensions: 32
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 495
Length of database: 486
Length adjustment: 34
Effective length of query: 461
Effective length of database: 452
Effective search space:   208372
Effective search space used:   208372
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory