Aligments for a candidate for patD in Marinobacter adhaerens HP15

GapMind for catabolism of small carbon sources

Aligments for a candidate for patD in Marinobacter adhaerens HP15

Align 4-(gamma-glutamylamino)butanal dehydrogenase (EC 1.2.1.99) (characterized)
to candidate GFF964 HP15_943 aldehyde dehydrogenase family protein

Query= BRENDA::P23883
         (495 letters)



>FitnessBrowser__Marino:GFF964
          Length = 505

 Score =  563 bits (1452), Expect = e-165
 Identities = 279/493 (56%), Positives = 360/493 (73%), Gaps = 6/493 (1%)

Query: 7   AYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVTQAPLAKIARGKSVDIDRAMSAA 66
           A WQ  A +L +E R ++NG Y  AA  E F +V P+    LA IA     D D+A+ AA
Sbjct: 14  AEWQALATNLTLEGRAYLNGTYQWAANGEAFTSVSPIDGRELASIASCDQSDADQAVMAA 73

Query: 67  RGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELALLETLDTGKPIRHSLRDDIPGAAR 126
           R  FE G WS  +PAKRKAVL + A+L+EAH +ELALLETLD GKPI H+   D+P  AR
Sbjct: 74  RAAFEAGIWSQLAPAKRKAVLLRFAELIEAHGDELALLETLDMGKPINHASNVDVPATAR 133

Query: 127 AIRWYAEAIDKVYGEVATTSSHELAMIVREPVGVIAAIVPWNFPLLLTCWKLGPALAAGN 186
           AIRW AEAIDKVYGE+A T  +++ MI REP+GV+AAIVPWNFP+++  WK+ PALA GN
Sbjct: 134 AIRWTAEAIDKVYGELAPTPHNQIGMISREPMGVVAAIVPWNFPMIMAAWKIAPALATGN 193

Query: 187 SVILKPSEKSPLSAIRLAGLAKEAGLPDGVLNVVTGFGHEAGQALSRHNDIDAIAFTGST 246
           SVILKPSEKSPLSAIRLA LA EAG+P GV NV+ G+GH  G+AL+ H D+D + FTGST
Sbjct: 194 SVILKPSEKSPLSAIRLAALAGEAGVPAGVFNVLPGYGHTVGKALALHMDVDCLVFTGST 253

Query: 247 RTGKQLLKDAGDSNMKRVWLEAGGKSANIVFADCPDLQQAASATAAGIFYNQGQVCIAGT 306
              KQL+  AG SNMKRVWLEAGGKS NIVFAD PDL++AA+  A+ I +NQG+VC AG+
Sbjct: 254 NVAKQLMIYAGQSNMKRVWLEAGGKSPNIVFADAPDLKKAAAEAASAIAFNQGEVCTAGS 313

Query: 307 RLLLEESIADEFLALLKQQAQNWQPGHPLDPATTMGTLIDCAHADSVHSFIREGESKGQL 366
           RLL+E SI  EF+ L+ +  + W+PGHPLDPATT G ++D A  D +  +I  G+S+G  
Sbjct: 314 RLLVENSIRAEFVRLICEALKTWRPGHPLDPATTCGAIVDQAQLDRIIDYIGIGQSEGAR 373

Query: 367 LLDG-----RNAGLAAAIGPTIFVDVDPNASLSREEIFGPVLVVTRFTSEEQALQLANDS 421
           L++G      N G    + PT+F  V+    ++ EEIFGPVL V  F + ++A+ +ANDS
Sbjct: 374 LVEGGQRILENTG-GLFVQPTVFDGVNNQMRIASEEIFGPVLSVIGFDTADEAVAIANDS 432

Query: 422 QYGLGAAVWTRDLSRAHRMSRRLKAGSVFVNNYNDGDMTVPFGGYKQSGNGRDKSLHALE 481
            YGL AAVWT +++ AH++++ L+AGSV++N+Y+ GDMT PFGG+KQSGNGRDKS+HA +
Sbjct: 433 IYGLAAAVWTSNINTAHKVAKALRAGSVWINHYDGGDMTAPFGGFKQSGNGRDKSVHAFD 492

Query: 482 KFTELKTIWISLE 494
           K+TELK  W+ LE
Sbjct: 493 KYTELKATWLVLE 505


Lambda     K      H
   0.317    0.133    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 675
Number of extensions: 18
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 495
Length of database: 505
Length adjustment: 34
Effective length of query: 461
Effective length of database: 471
Effective search space:   217131
Effective search space used:   217131
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources