GapMind for catabolism of small carbon sources

 

L-asparagine catabolism in Marinobacter adhaerens HP15

Best path

ans, glt

Also see fitness data for the top candidates

Rules

Overview: Asparagine catabolism in GapMind is based on asparaginase, which forms ammonia and aspartate. The asparaginase may be secreted or cytoplasmic. Asparatate can be transaminated to oxaloacetate, which is an intermediate in central metabolism.

34 steps (24 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
ans asparaginase HP15_1914
glt aspartate:proton symporter Glt HP15_2676
Alternative steps:
aapJ ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), substrate-binding component AapJ HP15_2196
aapM ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), permease component 2 (AapM) HP15_2194
aapP ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), ATPase component AapP HP15_2193 HP15_2920
aapQ ABC transporter for amino acids (Asp/Asn/Glu/Pro/Leu), permease component 1 (AapQ) HP15_2195
aatJ aspartate/asparagine ABC transporter, substrate-binding component AatJ
aatM aspartate/asparagine ABC transporter, permease component 2 (AatM) HP15_2919 HP15_2194
aatP aspartate/asparagine ABC transporter, ATPase component HP15_2193 HP15_2920
aatQ aspartate/asparagine ABC transporter, permease component 1 (AatQ) HP15_2919 HP15_3033
acaP aspartate permease AcaP
agcS Probable asparagine:Na+ symporter AgcS HP15_3907
AGP1 L-asparagine permease AGP1
ansP L-asparagine permease AnsP
bgtA aspartate ABC transporter, ATPase component BgtA HP15_2193 HP15_2920
bgtB' aspartate ABC transporter, permease component 1 (BgtB) HP15_2195
BPHYT_RS17540 aspartate:H+ (or asparagine) symporter
bztA aspartate/asparagine ABC transporter, substrate-binding component BztA HP15_2196
bztB aspartate/asparagine ABC transporter, permease component 1 (BztB) HP15_2195
bztC aspartate/asparagine ABC transporter, permease component 2 (BztC) HP15_2194
bztD aspartate/asparagine ABC transporter, ATPase component (BztD) HP15_2193 HP15_2920
dauA dicarboxylic acid transporter DauA HP15_3240
glnP L-asparagine ABC transporter, fused permease and substrate-binding components GlnP
glnQ L-asparagine ABC transporter, ATPase component GlnQ HP15_2920 HP15_3030
natF aspartate ABC transporter, substrate-binding component NatF HP15_2196
natG aspartate ABC transporter, permease component 1 (NatG) HP15_2919
natH aspartate ABC transporter, permease component 2 (NatH) HP15_2194 HP15_2919
peb1A aspartate ABC transporter, perisplasmic substrate-binding component Peb1A
peb1B aspartate ABC transporter, permease component 1 (Peb1B) HP15_2919
peb1C aspartate ABC transporter, ATPase component Peb1C HP15_2193 HP15_2920
peb1D aspartate ABC transporter, permease component 2 (Peb1D) HP15_2919 HP15_2195
SLC7A13 sodium-independent aspartate transporter
yhiT probable L-asparagine transporter YhiT
yveA aspartate:proton symporter YveA

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory