GapMind for catabolism of small carbon sources

 

Alignments for a candidate for peb1C in Marinobacter adhaerens HP15

Align PEB1C, component of Uptake system for glutamate and aspartate (characterized)
to candidate GFF1457 HP15_1422 amino acid ABC transporter ATP-binding protein

Query= TCDB::A3ZI83
         (242 letters)



>FitnessBrowser__Marino:GFF1457
          Length = 240

 Score =  213 bits (541), Expect = 4e-60
 Identities = 112/241 (46%), Positives = 168/241 (69%), Gaps = 4/241 (1%)

Query: 1   MIELKNVNKYYGTHHVLKNINLSVKEGEKLVIIGPSGSGKSTTIRCMNGLEEVSSGEVVV 60
           MI ++NV+K +G   VLK ++L VK+GE + +IG SGSGKST + C+N +E ++ G+++V
Sbjct: 1   MISVQNVHKSFGDLEVLKGVSLDVKKGEVVSVIGGSGSGKSTLLYCINAIEPINKGKILV 60

Query: 61  NNLVLNHK--NKIEICRKYCAMVFQHFNLYPHMTVLQNLTLAPMKLQKKSKKEAEETAFK 118
           +++ ++ K  NK ++ R+   MVFQ +N +PHMTVL+N  LAP  ++K SK+EA E A K
Sbjct: 61  DDVDVHAKETNKDKL-RQKLGMVFQQWNSFPHMTVLENAALAPRIVKKMSKEEAIEIAKK 119

Query: 119 YLKVVGLLDKANVYPATLSGGQQQRVAIARSLCTKKPYILFDEPTSALDPETIQEVLDVM 178
            L+ VGL DK +VYP  +SGGQQQR+AIAR+L  K  Y+L DE TSALDPE + EVLD +
Sbjct: 120 ELEHVGLGDKFDVYPTKMSGGQQQRLAIARALAMKPDYMLLDEVTSALDPELVGEVLDTL 179

Query: 179 KEISHQSNTTMVVVTHEMGFAKEVADRIIFMEDGAIVEENIPSEFFSNPKTERARLFLGK 238
           + ++ +   TM+ VTHEMGFA++V+DR+ +  +G I E     +  ++P+    + FL K
Sbjct: 180 RLLA-EEGMTMICVTHEMGFARDVSDRVAYFHEGVIAEIGEARQVITDPQNPLTQKFLSK 238

Query: 239 I 239
           +
Sbjct: 239 V 239


Lambda     K      H
   0.317    0.133    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 136
Number of extensions: 5
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 242
Length of database: 240
Length adjustment: 23
Effective length of query: 219
Effective length of database: 217
Effective search space:    47523
Effective search space used:    47523
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory