Align ABC transporter for L-Arginine and L-Citrulline, permease component 1 (characterized)
to candidate GFF3090 HP15_3033 amino acid ABC transporter, inner membrane subunit
Query= reanno::pseudo3_N2E3:AO353_03045 (232 letters) >FitnessBrowser__Marino:GFF3090 Length = 240 Score = 231 bits (589), Expect = 9e-66 Identities = 113/226 (50%), Positives = 162/226 (71%), Gaps = 2/226 (0%) Query: 6 NVIWEALPL--YFGGLVTTLKLLALSLLFGLLAALPLGLMRVSKQPIVNMSAWLYTYVIR 63 N I+ A+ + Y+ G+VTT+ L+ LSL+ GLL A+PL ++R + P V+ WLYTY+ R Sbjct: 12 NEIFTAMTIMEYWDGMVTTVHLVFLSLVIGLLVAVPLAILRTVRNPFVSGPVWLYTYLFR 71 Query: 64 GTPMLVQLFLIYYGLAQFEAVRESFLWPWLSSATFCACLAFAINTSAYTAEIIAGSLRAT 123 GTP+L+QL++IYYGLAQ E ++E+F W + A LAF +NT+AYT EII G++ +T Sbjct: 72 GTPLLIQLYIIYYGLAQIEGIQETFWWEIFREPFYPALLAFTLNTAAYTTEIIRGAIIST 131 Query: 124 PNGEIEAAKAMGMSRFKMYKRILLPSALRRALPQYSNEVIMMLQTTSLASIVTLIDITGA 183 PNGEIEAAKA GM+ F +RI+LPSA RRA+ YSNEVI ML +++AS+VT++D+TGA Sbjct: 132 PNGEIEAAKAYGMNWFMRMRRIVLPSAARRAVQAYSNEVIFMLHASAIASVVTIVDLTGA 191 Query: 184 ARTVNAQYYLPFEAYITAGVFYLCMTFILVRLFKMAEHRWLGYLAP 229 AR + +++Y PF+A+I + Y+ +TFILV F+ E L + P Sbjct: 192 ARNIYSRFYAPFDAFIFVALCYMALTFILVFAFRKLETHLLKHQRP 237 Lambda K H 0.329 0.139 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 156 Number of extensions: 5 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 232 Length of database: 240 Length adjustment: 23 Effective length of query: 209 Effective length of database: 217 Effective search space: 45353 Effective search space used: 45353 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory