GapMind for catabolism of small carbon sources

 

Alignments for a candidate for deoxyribonate-dehyd in Marinobacter adhaerens HP15

Align 2-deoxy-D-ribonate dehydrogenase (characterized)
to candidate GFF846 HP15_825 short-chain dehydrogenase/reductase SDR

Query= metacyc::MONOMER-20835
         (262 letters)



>FitnessBrowser__Marino:GFF846
          Length = 256

 Score =  109 bits (273), Expect = 5e-29
 Identities = 74/250 (29%), Positives = 122/250 (48%), Gaps = 13/250 (5%)

Query: 12  GLRVLISGGAAGIGEVLAAAYLEAGAQVHVC----DVSESALAVFRDKYPGTVATRADVS 67
           G   LI+G + GIGE +A    E GA V V     D  E+  +  RD      A    + 
Sbjct: 11  GKVALITGASRGIGESIARTLAEYGAHVIVSSRKIDGCEAVASSIRDAGGSAEAYACHIG 70

Query: 68  DAAQIEAVFKVQREHLGGLDVLVNNAGIAGPTGGIDAISDAEWQATININLTAQYRFAHH 127
           D  QIE+++    +  G LD+LVNNA      G ++      +  T+++N+   +     
Sbjct: 71  DMDQIESIWAHIDQTHGKLDILVNNAAANPYFGPVEDTDLGAFNKTVDVNIRGYFFMCAR 130

Query: 128 AVPMLKESSHGHLLHIASVAGRLGYAWRTPYAATKWAIVGLMKSLASELGESDIRVNALL 187
              M+K++  G ++++ASV G     ++  Y+ TK A++ + KS A ELG+  IRVNALL
Sbjct: 131 GAQMMKKAGGGSIVNVASVNGVNPGHYQGIYSVTKAAVISMTKSFAMELGQQKIRVNALL 190

Query: 188 PGIVEGPRMDGVIRARAEQVGVPEAEMRQEYLNKISLKRMVTAEDVAAMALFLCSPAARN 247
           PG+ +      +    A         ++++ +  I +KR+    ++A   L+L S A+  
Sbjct: 191 PGLTDTKFASALTTNEA---------IKKQAMAHIPMKRVADPGEMAGTVLYLVSDASSY 241

Query: 248 VTGQAISVDG 257
            TG  I+ DG
Sbjct: 242 TTGACINADG 251


Lambda     K      H
   0.318    0.133    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 162
Number of extensions: 6
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 262
Length of database: 256
Length adjustment: 24
Effective length of query: 238
Effective length of database: 232
Effective search space:    55216
Effective search space used:    55216
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory