Alignments for a candidate for drdehyd-beta in Marinobacter adhaerens HP15

GapMind for catabolism of small carbon sources

Alignments for a candidate for drdehyd-beta in Marinobacter adhaerens HP15

Align 2-deoxy-D-ribose dehydrogenase β subunit (characterized)
to candidate GFF1288 HP15_1258 isoquinoline 1-oxidoreductase, beta subunit

Query= metacyc::MONOMER-20833
         (739 letters)



>FitnessBrowser__Marino:GFF1288
          Length = 734

 Score =  394 bits (1011), Expect = e-113
 Identities = 260/748 (34%), Positives = 377/748 (50%), Gaps = 48/748 (6%)

Query: 14  LSRRRFL--ASTAVGALVIGFGLPLGAGRVQAATSAERGTQVP-AFLEIRPDGTVRLLSP 70
           ++RR FL  ++ A G+L++   LP G   +Q     + G   P A+LE+  D  V     
Sbjct: 3   MNRRNFLKVSAGASGSLMVSITLP-GCAGIQTGYEPDTGEWRPDAWLELTADDEVFFTLA 61

Query: 71  FMEGGQGTHTAMAQIVGEELDADPATFIVEAAPPGEAYVVMENGLRITGGSMSVRMSYPT 130
            +E GQGT+T +  ++ EEL+  P       AP    Y      L++TGGS SV  S+  
Sbjct: 62  RVEMGQGTYTGLTTLIAEELEIQPERITPRFAPVAPEYRNPLYKLQLTGGSTSVATSWEP 121

Query: 131 MRRLGALARAMLLQAGAKQWGVPVGELTTQPGRVVHAASGRSLGYGELASSALDMPVPDP 190
           +R  GA AR ML+ A A+ W V   E     GRVVH     S+ YG+L   A    +   
Sbjct: 122 LRTAGAEARQMLVMAAARVWNVEATECRASEGRVVHPNGIDSMAYGQLVELAAKEVLR-- 179

Query: 191 ASITLRDPSQFRWIGKPVKRLDAYDKSTGKALYSIDLKVDNMLHAAVQHAPRLGMTVGSL 250
             I L+  S++++IGK   +LDA  KSTG A+Y ID+ + +M++  V   PR G  V   
Sbjct: 180 GDIALKPQSEWKYIGKQRGKLDARAKSTGTAVYGIDVDLPDMVYGVVSRPPRRGGRVRDF 239

Query: 251 RNQSQVEGMKGVHSVHVLPGAVAVVAERWWHAKRAVEAIQVDWQEAAADSALRVMPADFS 310
            N  QV  M GV  V  +   VAVVA+++W A++A +A+++DW  + A S    +     
Sbjct: 240 -NADQVRAMTGVLDVFKIERGVAVVADKYWRARKAQDALKIDWDFSDAVS----LSTGDV 294

Query: 311 SDKHREFLAAQQGPTRDDENEGDVAGALKGAKTQVEATYHNQYLNHAQLEPPSALARFNP 370
            D +R   AA   P   + +EG  A A++     VEA Y   YL HA LEP +A A +  
Sbjct: 295 FDSYRA--AADDDPGESERSEGSFASAMEEGDRVVEAEYAQPYLAHATLEPMNATAWYRE 352

Query: 371 DGTLEIWLPNQAPDMFRADIAKRTGLDPAQITLHSPLLGGFFGRHFLYDSANPYPQAIAL 430
           DG +E+W P QAPD+ R   A+ T L P  IT+++  LGG FGR    D      +A A+
Sbjct: 353 DG-MEVWAPTQAPDLGRIAAARVTNLGPDDITINTTFLGGGFGRRLTQDYIE---EAAAV 408

Query: 431 AKAVSRPIKLIWSREEEFLRDVLRPLAVVKFRAALDDKGL-------------------- 470
           A  V RP+K+IWSREE+   D+ RP  + + +A+L    L                    
Sbjct: 409 AYRVGRPVKVIWSREEDTRHDLYRPAMLHRMKASLSGAELKGWHHQIVGPQILDWYVRNA 468

Query: 471 -----PVAIEAVSATEGPTEALAGKQGDKIDPTAVEGLSGKSYAIPNKRIAQIYVKGAPM 525
                P A + +  T G    +A       D +A+EG     Y +PN  I   +      
Sbjct: 469 APAQYPWAPKFMYDTLGSVGLMAEGIATPKDHSAIEGAIEYPYRVPNIDIRHTHTDPGIP 528

Query: 526 LGYWRSVGNSLNDFFYESFLDELADKGGHDPYELRLHLLRDNPRLTTLLQAAGELSGGWK 585
           + +WRSVG S N F  E+F+DELA + G DPY  R  L+   PR   +L     L+    
Sbjct: 529 ITWWRSVGFSHNGFAVETFMDELAHEAGEDPYRFRRRLIAHEPRHLEVLDRVVRLADWQS 588

Query: 586 RGPFTAEDGTRRARGVAMASPFGSHTAAIAEVSIENGKVKVHDIWQAIDPGSIVNPAIVE 645
             P        RARG+A+   FG++ A + E  IENG ++V+ +  ++D G +VNP IVE
Sbjct: 589 PVP------EGRARGLALFRSFGTYVAQVVEAGIENGAIRVYKVTCSVDCGQVVNPRIVE 642

Query: 646 AQVNGAVALGLSQTLLEEAVYVDGKPRARNYDLYPILAPAQMARVHVRVVESGEKMGGIG 705
            Q+ G +  GL+  L  E    +G+ +  N+  Y ++   +   V V +V S E   G+G
Sbjct: 643 DQIEGGILFGLTAALYGEITLENGEVQQSNFHDYRLMRQYERPEVLVDIVASSESPTGVG 702

Query: 706 EPPLPAVAPAVANAVAQLTGQRVRSLPL 733
           EP +P V PA+ NA+  L G+R RSLPL
Sbjct: 703 EPGVPPVIPALGNALFALNGKRQRSLPL 730


Lambda     K      H
   0.317    0.134    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1293
Number of extensions: 64
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 739
Length of database: 734
Length adjustment: 40
Effective length of query: 699
Effective length of database: 694
Effective search space:   485106
Effective search space used:   485106
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources