Aligments for a candidate for etoh-dh-nad in Marinobacter adhaerens HP15

GapMind for catabolism of small carbon sources

Aligments for a candidate for etoh-dh-nad in Marinobacter adhaerens HP15

Align alcohol dehydrogenase (EC 1.1.1.1) (characterized)
to candidate GFF2856 HP15_2800 glucose-methanol-choline oxidoreductase

Query= BRENDA::Q76HN6
         (526 letters)



>lcl|FitnessBrowser__Marino:GFF2856 HP15_2800
           glucose-methanol-choline oxidoreductase
          Length = 537

 Score =  548 bits (1413), Expect = e-160
 Identities = 286/538 (53%), Positives = 363/538 (67%), Gaps = 14/538 (2%)

Query: 1   MEFDYLIVGAGSAGCVLANRLSADPSVTVCLLEAGPEDRSPLIHTPLGLAAILPTRHV-- 58
           M FDY+IVG GSAG V+A RLS DP V+VCLLEAG +    L   P G+ AI+P      
Sbjct: 1   MIFDYIIVGGGSAGAVMAARLSEDPDVSVCLLEAGGKGDHLLTRAPAGVVAIMPGHGKIN 60

Query: 59  NWAFKTTPQPGLGGRVGYQPRGKVLGGSSSINGMIYIRGHQDDFNDWQALGNEGWGFDDV 118
           NWA  T  QP L GR G+QPRG+ LGGSS IN M+Y+RGH  D++ W  LG +GWG+DDV
Sbjct: 61  NWALNTEQQPELAGRRGFQPRGRGLGGSSLINAMLYVRGHSADYDGWAELGCDGWGWDDV 120

Query: 119 LPYFRKSEMHHGGSSEYHGGDGELYV-SPANRHAASEAFVESALRAGHSYNPDFNGATQE 177
           LPYFRK+E H GG+SEYHG DG L+V    +    SEAF+E+A   G+  + DFN    E
Sbjct: 121 LPYFRKAECHEGGASEYHGADGPLHVCKQRSPRPISEAFIEAAKERGYPASEDFNTGDNE 180

Query: 178 GAGYYDVTI-----RDGRRWSTATAFLKPV-RHRSNLTVLTHTHVESIVLLGKQATGVQA 231
           G G ++VT      R+G R STA A+L P+   R+NL V+T      I+  GK+A+GV+ 
Sbjct: 181 GVGLFEVTQFHDTERNGERCSTAAAYLYPIIEQRNNLKVVTGARATRILFNGKRASGVEY 240

Query: 232 LIKGSRVHLRARKEVILSAGAFGSPHLLMLSGIGSAAELEPQGIAPRHELPGVGQNLQDH 291
            +KG  +   A +EVILSAGAFGSP LL LSG+G+  ++ P GI   HELPGVG+NLQDH
Sbjct: 241 RLKGQSLTASANREVILSAGAFGSPQLLQLSGVGNPDDILPHGIPMVHELPGVGRNLQDH 300

Query: 292 ADVVLCYKSNDTSLLGFSLSGGVKMGKAMFDYARHRNGPVASNCAEAGAFLKTDPGLERP 351
            D +L YKS DT   GFSL+G   M +    +     G +AS  AE  AFLK+DP  ++P
Sbjct: 301 LDFILAYKSADTDNFGFSLTGMKNMLRHSLQWRTDGTGMIASPFAEGAAFLKSDPEQDKP 360

Query: 352 DIQLHSVIGTVDDHNRKLHWGHGFSCHVCVLRPKSIGSVGLASPDPRKAPRIDPNFLAHD 411
           D+QLH V+  V+DH RKLHWGHGFSCHVC LRPKS G V L S DP   P IDPN+L+  
Sbjct: 361 DLQLHFVVSIVEDHARKLHWGHGFSCHVCNLRPKSRGRVFLLSADPMADPGIDPNYLSDP 420

Query: 412 DDVATLLKGYRITRDIIAQTPMASFGLRDMYSAGLH---NDEQLIELLRKRTDTIYHPIG 468
           DD+   +KG +ITR+I+    ++ +   +M+  G+H   +DE+    +R R DTIYHP+G
Sbjct: 421 DDLNLTIKGAKITREILEGPALSPYRQSEMF--GVHDGMSDEEWERHIRARADTIYHPVG 478

Query: 469 TCKMGQDEMAVVDSQLRVHGIEGLRVVDASIMPTLVGGNTNAAAIMIAERAAEWIAHG 526
           TCKMG D++AVVDS L+VHG+EGLRVVDAS+MPTL+GGNTNA  IMIAE+ A+ I  G
Sbjct: 479 TCKMGIDDLAVVDSSLKVHGLEGLRVVDASVMPTLIGGNTNAPTIMIAEKNADTIRTG 536


Lambda     K      H
   0.319    0.137    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 950
Number of extensions: 34
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 526
Length of database: 537
Length adjustment: 35
Effective length of query: 491
Effective length of database: 502
Effective search space:   246482
Effective search space used:   246482
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources