Alignments for a candidate for Dshi_1194 in Marinobacter adhaerens HP15

GapMind for catabolism of small carbon sources

Alignments for a candidate for Dshi_1194 in Marinobacter adhaerens HP15

Align TRAP transporter (characterized, see rationale)
to candidate GFF1092 HP15_1070 TRAP-type uncharacterized transport system, fused permease component

Query= uniprot:A8LI82
         (743 letters)



>FitnessBrowser__Marino:GFF1092
          Length = 867

 Score =  216 bits (550), Expect = 4e-60
 Identities = 115/290 (39%), Positives = 174/290 (60%), Gaps = 7/290 (2%)

Query: 185 DLVLAICGVAVATYLITIYGTLMRNSTGTPFAPIGISI-AAVAGTALIMELTRRVAGMAL 243
           D +LA+     A+YL   Y  L       P API   +  A+ G  L++E TRR  G+ L
Sbjct: 102 DWILALVAAFCASYLYVFYEELSTR----PGAPITQDLWVALIGLVLLLEATRRSLGLPL 157

Query: 244 IVIAGIFLAYVFVGQYLPGFLNAPAVTWQRFFSQVYTDA-GILGPTTAVSSTYIILFIIF 302
            ++A +F+ Y   G Y+P  +    V+  +  S  +    G+ G    VS++++ LF++F
Sbjct: 158 TIVAAVFITYSIAGPYMPDVIAHKGVSLSKLASHQWLGTEGVFGVALGVSTSFVFLFVLF 217

Query: 303 AAFLQASKVGDYFVNFAFAAAGQSRGGPAKVAIFASGLMGMINGTSAGNVVATGSLTIPL 362
            A L+ +  G+YF+  A+A  G  RGGPAK A+ +SGL G+I+G+S  NVV TG+ TIPL
Sbjct: 218 GALLERAGAGNYFIKVAYAMLGHMRGGPAKAAVVSSGLSGVISGSSIANVVTTGTFTIPL 277

Query: 363 MKKVGYHKKTAGAVEAAASTGGQIMPPIMGAGAFIMAEITGIPYTEIALAAIIPAILYFV 422
           MK+VG+    AGAVE AAST GQ+ PPIMGA AF+M E  GI Y E+   AI+PA++ +V
Sbjct: 278 MKRVGFPATKAGAVEVAASTNGQLTPPIMGAAAFLMVEYVGISYLEVIKHAILPAMISYV 337

Query: 423 SVYFMVDLEAAKLGMRGMSRDELPKF-NKMVRQVYLFLPIIILIYALFMG 471
           ++ ++V LEA KL M+G+ R   P    +++  V + + + +L  A++ G
Sbjct: 338 ALIYIVHLEACKLNMQGIERLNKPTLAQRLLNWVVILIGLSVLTLAVYYG 387



 Score =  152 bits (384), Expect = 7e-41
 Identities = 93/268 (34%), Positives = 143/268 (53%), Gaps = 26/268 (9%)

Query: 444 ELPKFNKMVRQ-VYLFLPIIILIYAL----------------FMGYSVIRAGTLATVAAA 486
           ELP     V+  +Y  LP+++L++ L                FM + V+    L      
Sbjct: 435 ELPPVGPTVKTGLYFLLPVVVLVWCLTVERFSPQLSAFWATLFMIFIVVTQRPLKAFFYK 494

Query: 487 VVSWFTPFRMGPRSIAKAFEIAGTMSVQIIAVCACAGIIVGVISLTGVGARFSAVLLGIA 546
             ++   F+ G   +A +        V I    A AGI+VG ++LTG+G   +  +  ++
Sbjct: 495 HGNFLGEFKDGVFDLAHSLATGARNMVGIGVATATAGIVVGTVTLTGIGLVMTEFVEFLS 554

Query: 547 DTSQLLALFFAMCIAILLGMGMPTTAAYAVAASVVAPGLVQLG------IPLLTAHFFVF 600
             + LL L F   I+++LGMG+PTTA Y V ++++AP +V LG      +PL+  H FVF
Sbjct: 555 GGNLLLMLIFTAIISLILGMGLPTTANYIVVSTLMAPVIVTLGAQNGLIVPLIAVHLFVF 614

Query: 601 YFAVLSAITPPVALASYAAAGISGANPMETSVTSFKIGIAAFIVPFMFFYNSAILMDGT- 659
           YF +L+  TPPV LA+YAAA ISGA+P+ T +  F   I   I+PFMF +N+ +L+ G  
Sbjct: 615 YFGILADDTPPVGLAAYAAAAISGADPIRTGIQGFTYDIRTAILPFMFIFNTQLLLIGLT 674

Query: 660 -WFEVLRAGATAVVGVFFLSSGVQG-WF 685
            WF++L    +AV  +   S+  QG WF
Sbjct: 675 GWFDLLVTIFSAVTAMLVFSAATQGFWF 702


Lambda     K      H
   0.327    0.140    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1658
Number of extensions: 98
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 4
Number of HSP's successfully gapped: 2
Length of query: 743
Length of database: 867
Length adjustment: 41
Effective length of query: 702
Effective length of database: 826
Effective search space:   579852
Effective search space used:   579852
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources