Alignments for a candidate for dauA in Marinobacter adhaerens HP15

GapMind for catabolism of small carbon sources

Alignments for a candidate for dauA in Marinobacter adhaerens HP15

Align C4-dicarboxylic acid transporter DauA; Dicarboxylic acid uptake system A (characterized)
to candidate GFF3298 HP15_3240 sulfate permease family protein

Query= SwissProt::P0AFR2
         (559 letters)



>FitnessBrowser__Marino:GFF3298
          Length = 572

 Score =  605 bits (1559), Expect = e-177
 Identities = 316/542 (58%), Positives = 399/542 (73%), Gaps = 11/542 (2%)

Query: 14  ALIDACWKEKYTAARFTRDLIAGITVGIIAIPLAMALAIGSGVAPQYGLYTAAVAGIVIA 73
           A  +AC  E Y   RF RD++AG+TVGIIAIPLAMALAI SGVAPQYGLYTA +AG VIA
Sbjct: 4   AFREACIDEPYRGRRFLRDVMAGLTVGIIAIPLAMALAIASGVAPQYGLYTAIIAGFVIA 63

Query: 74  LTGGSRFSVSGPTAAFVVILYPVSQQFGLAGLLVATLLSGIFLILMGLARFGRLIEYIPV 133
           LTGGSRFS+SGPTAAFVVILYP++Q +GL GLL+ATL+SG+ LILM L R GR IEYIP 
Sbjct: 64  LTGGSRFSISGPTAAFVVILYPIAQSYGLGGLLLATLMSGVLLILMALMRLGRFIEYIPE 123

Query: 134 SVTLGFTSGIGITIGTMQIKDFLGLQMAHVPEHYLQKVGALFMALPTINVGDAAIGIVTL 193
           SVTLGFT GI + I T+QI+DFLGLQ++ +PEHY  K+  L  ALP  +   A +  VTL
Sbjct: 124 SVTLGFTGGIAVVIATLQIRDFLGLQVSDMPEHYWDKLALLAGALPEFDGMSALVAGVTL 183

Query: 194 GILVFWPRLGIRLPGHLPALLAGCAVMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQL 253
             ++ WPRL   +P HLPA++ G  +   +N  G  + TIGS+F Y+L DG++G GIP  
Sbjct: 184 ACMLLWPRLKTPVPPHLPAVVIGSLLALWLNAQGAAIDTIGSRFSYLLPDGTEGAGIPPF 243

Query: 254 LPQLVLPWDLPNSE---FTLTWDSIRTLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKA 310
           LP+   PW    +      L+W  +R LLPAAF++AMLGAIESLLCAVVLDGMTG +H A
Sbjct: 244 LPEFAWPWQQAGASGEPVGLSWSLVRDLLPAAFAIAMLGAIESLLCAVVLDGMTGKRHSA 303

Query: 311 NSELVGQGLGNIIAPFFGGITATAAIARSAANVRAGATSPISAVIHSILVILALLVLAPL 370
           NSEL+GQGLGNII PFFGGITATAAIARSAAN RAGA SP+SA++HS++V+LAL+ LA L
Sbjct: 304 NSELMGQGLGNIITPFFGGITATAAIARSAANYRAGAESPVSAMVHSLVVLLALVSLAGL 363

Query: 371 LSWLPLSAMAALLLMVAWNMSEAHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVG 430
           L++LP+ AMAALL+MVAWNMSEA K + LL+ AP+ DI+V L C SLTVL DMVIAI+ G
Sbjct: 364 LAYLPMPAMAALLVMVAWNMSEAPKSLHLLKTAPRSDILVFLTCFSLTVLLDMVIAITTG 423

Query: 431 IVLASLLFMRRIARMTRLAPVVVD-------VPDDVLVLRVIGPLFFAAAEGLFTDLESR 483
           ++LA++LFMR +A+MTR+  +          +PD   V ++ GPLFFAAA+ +F +L   
Sbjct: 424 VLLAAVLFMREMAQMTRVTDITQSKRIAESRLPDGWQVFKINGPLFFAAADRIFGELAVL 483

Query: 484 LEGKRIVILKWDAVPVLDAGGLDAFQRFVKRLP-EGCELRVCNVEFQPLRTMARAGIQPI 542
               R  IL  D V +LDAGGL A  + +     +G ++ + +++FQPLRT+ARAG+ PI
Sbjct: 484 ARNARGFILYMDGVTILDAGGLSALNKLIATCERDGTQIVIADLQFQPLRTLARAGVAPI 543

Query: 543 PG 544
            G
Sbjct: 544 AG 545


Lambda     K      H
   0.328    0.142    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 798
Number of extensions: 36
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 559
Length of database: 572
Length adjustment: 36
Effective length of query: 523
Effective length of database: 536
Effective search space:   280328
Effective search space used:   280328
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources