Align Acetoacetate--CoA ligase (EC 6.2.1.16) (characterized)
to candidate GFF1020 HP15_999 acyl-CoA synthase
Query= reanno::acidovorax_3H11:Ac3H11_3009 (578 letters) >FitnessBrowser__Marino:GFF1020 Length = 548 Score = 645 bits (1663), Expect = 0.0 Identities = 317/553 (57%), Positives = 399/553 (72%), Gaps = 7/553 (1%) Query: 24 VPLIEQTIGAFFADMVARQPEREALVSVHQGRRYTYAQLQTEAHRLASALLGMGLTPGDR 83 +PL+ TIG + P+ EALV +HQ R+TY + + A A + +G+ GDR Sbjct: 1 MPLLGMTIGEMLDRTAEKYPDNEALVCLHQDIRWTYKEFVEKVDEAARAFMAIGVKRGDR 60 Query: 84 VGIWSHNNAEWVLMQLATAQVGLVLVNINPAYRTAEVEYALNKVGCKLLVSMARFKTSDY 143 VGIWS N EW + Q ATA+VG +LVNINPAY E++YALN G LV+ FK S+Y Sbjct: 61 VGIWSPNRYEWTVTQFATAKVGAILVNINPAYGVHELQYALNLAGITTLVTADSFKASNY 120 Query: 144 LGMLRELAPEWQGQQPGHLQAAKLPQLKTVVWIDDEAGQGADEPGLLRFTELIA-RGNAA 202 M+ ELAPE + PG L+A +P+L+ V+ + ++ G+ + E + + + Sbjct: 121 REMIYELAPELKRSSPGKLKADHVPELRAVINVHEDK-----HDGMWTWKEFLGFSSDVS 175 Query: 203 DPRLAQVAAGLQATDPINIQFTSGTTGFPKGATLTHRNILNNGFFIGECMKLTPADRLCI 262 L + LQ DPINIQFTSGTTG PKGATLTH NILNNG+F+GE LT DRL I Sbjct: 176 QDDLVKRQGELQFDDPINIQFTSGTTGNPKGATLTHHNILNNGYFVGESQLLTEKDRLVI 235 Query: 263 PVPLYHCFGMVLGNLACFTHGATIVYPNDGFDPLTVLQTVQDERCTGLHGVPTMFIAELD 322 PVPLYHCFGMV+GNL C THG+T++YP +GF+P +VLQ V E+ T L+GVPTMFIAEL Sbjct: 236 PVPLYHCFGMVMGNLGCITHGSTMIYPGEGFEPKSVLQAVHQEKATALYGVPTMFIAELA 295 Query: 323 HPRFAEFNLSTLRTGIMAGSPCPTEVMKRVVEQMNLREITIAYGMTETSPVSCQSSTDTP 382 P F ++LS+LRTGIMAGS CP EVMK+V +MN++E+ IAYGMTETSPVS Q+S+ P Sbjct: 296 EPEFETYDLSSLRTGIMAGSICPAEVMKKVNGKMNMKEVQIAYGMTETSPVSTQTSSLDP 355 Query: 383 LSKRVSTVGQVQPHLEVKIVDPDTGAVVPIGQRGEFCTKGYSVMHGYWGDEAKTREAIDE 442 K+V+TVG+ QPHLE KIVDP TG VVP G+ GE CT+GYSVM YW +E KTREAID Sbjct: 356 FEKQVTTVGRTQPHLETKIVDPGTGNVVPRGEIGELCTRGYSVMLKYWNNEEKTREAIDS 415 Query: 443 GGWMHTGDLATMDAEGYVNIVGRIKDMVIRGGENIYPREIEEFLYRHPQVQDVQVVGVPD 502 GWMHTGDLATMD EGYV IVGRIKDMVIRGGENIYP+EIEEFLY HP +++VQV G+PD Sbjct: 416 AGWMHTGDLATMDEEGYVQIVGRIKDMVIRGGENIYPKEIEEFLYTHPAIEEVQVTGIPD 475 Query: 503 QKYGEELCAWIIAKPGTQP-TEDDIRAFCKGQIAHYKVPRYIRFVTSFPMTVTGKIQKFK 561 KYGEEL AW+ P P T +D++AFCKG+IAH+K+P+ +FV FPMTVTGKIQKFK Sbjct: 476 DKYGEELIAWVKLAPDAAPVTAEDLQAFCKGKIAHFKIPKNYKFVDEFPMTVTGKIQKFK 535 Query: 562 IRDEMKDQLGLEE 574 +R+ +++GL++ Sbjct: 536 MREISIEEMGLKK 548 Lambda K H 0.320 0.136 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 869 Number of extensions: 28 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 578 Length of database: 548 Length adjustment: 36 Effective length of query: 542 Effective length of database: 512 Effective search space: 277504 Effective search space used: 277504 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory