GapMind for catabolism of small carbon sources

 

Aligments for a candidate for amaB in Marinobacter adhaerens HP15

Align Δ1-piperideine-6-carboxylate dehydrogenase (characterized)
to candidate GFF2711 HP15_2655 methylmalonate-semialdehyde dehydrogenase

Query= metacyc::MONOMER-12387
         (496 letters)



>lcl|FitnessBrowser__Marino:GFF2711 HP15_2655
           methylmalonate-semialdehyde dehydrogenase
          Length = 503

 Score =  199 bits (507), Expect = 1e-55
 Identities = 139/390 (35%), Positives = 195/390 (50%), Gaps = 11/390 (2%)

Query: 38  HPLTGADLFGLRAHTPEDVDRAVEAAHTAFLTWRTTPAPVRGALVKRFGELLTEHKQDLA 97
           +P TG     +     EDVD AV AA  AF  W  TP   R  ++ +F ELL  HK DLA
Sbjct: 27  NPATGQVTGRVALAGREDVDAAVAAADAAFPAWADTPPIRRARVMFKFLELLNTHKDDLA 86

Query: 98  DLVTIEAGKIRSEALGEVQEMIDICDFAVGLSRQLYGRTMPSERPGHRLMETWHPLGVVG 157
             +T E GK+ ++A GEV   IDI +FA G+ + L G        G     T  PLGVV 
Sbjct: 87  RAITAEHGKVFTDAQGEVARGIDIVEFACGIPQLLKGDYTEQVSTGIDNWTTRQPLGVVA 146

Query: 158 VISAFNFPVAVWAWNAAVALVCGDTVVWKPSELTPLNRAACAALLDLAIADAGAPKGLNQ 217
            ++ FNFP  V  W   VA+  G+T V KPS   PL+ +A   + DL +  AG P G+  
Sbjct: 147 GVTPFNFPAMVPMWMFPVAIAAGNTFVLKPS---PLDPSASLMIADL-LKQAGLPDGVFN 202

Query: 218 VVVGAADVGERLVDSPRVPLVSATGSTRMGRAVGPRVAARFGRTILELGG-NNAAVVTPS 276
           VV G  D    L++ P V  +S  GST +   +  +  A+ G+ I  LGG  N  VV P 
Sbjct: 203 VVQGDKDAVNALIEHPDVQALSFVGSTPIANLLYEK-GAKHGKRIQALGGAKNHMVVMPD 261

Query: 277 ADLDLTVNAAVFAAAGTAGQRCTTLRRLIVHEDIADTVVERLTAAFERLPIGDPFQDTTL 336
           ADLD  V+A + AA G+AG+RC  +   ++  D+AD +V RL      L + +  Q    
Sbjct: 262 ADLDKAVDALIGAAYGSAGERCMAISVAVLVGDVADKIVPRLAERARSLKVKNGEQLDAE 321

Query: 337 VGPLVNEAAFGRMREAVERATAEGGTLCAGGE----RQFPDAAPGAYYVRPALV-RMPAQ 391
           +GP+V  AA  R+   +++  AEG  L   G         D     +++  +L   +   
Sbjct: 322 MGPIVTAAAHQRITGYIDKGVAEGAELVVDGRGFDASNTGDGCADGFWMGGSLFDHVTPD 381

Query: 392 TAVVREETFAPILYVLTYRDLDEAIRLNNE 421
             + REE F P+L  +   D+  AIRL N+
Sbjct: 382 MTIYREEIFGPVLACVRVPDIATAIRLIND 411


Lambda     K      H
   0.320    0.135    0.406 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 515
Number of extensions: 28
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 496
Length of database: 503
Length adjustment: 34
Effective length of query: 462
Effective length of database: 469
Effective search space:   216678
Effective search space used:   216678
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory