GapMind for catabolism of small carbon sources

 

Aligments for a candidate for Ch1CoA in Marinobacter adhaerens HP15

Align Cyclohex-1-ene-1-carbonyl-CoA dehydrogenase; Ch1CoA; EC 1.3.8.10 (characterized)
to candidate GFF2 HP15_2 acyl-CoA dehydrogenase domain protein

Query= SwissProt::Q2LQN9
         (414 letters)



>lcl|FitnessBrowser__Marino:GFF2 HP15_2 acyl-CoA dehydrogenase
           domain protein
          Length = 389

 Score =  246 bits (627), Expect = 1e-69
 Identities = 142/377 (37%), Positives = 212/377 (56%), Gaps = 6/377 (1%)

Query: 37  LTEEQKLLMEMVRNLAVREIAPRAIEIDENHSFPVHARDLFADLGLLSPLVPVEYGGTGM 96
           L E   +L E +   A  EIAPRA EID N+ FP+       D+GLL   V  EYGG+ M
Sbjct: 12  LGETLDMLREQINGFAASEIAPRAEEIDRNNEFPMDLWRKMGDMGLLGITVSEEYGGSDM 71

Query: 97  DITTFAMVLEEIGKVCASTALMLLAQADGMLSII-LDGSPALKEKYLPRF--GEKSTLMT 153
                 + +EEI +  AS  L   A ++  ++ I  +G+   K+KYLP+   GE    + 
Sbjct: 72  GYLAHVIAMEEISRASASVGLSYGAHSNLCVNQIHRNGTEEQKQKYLPKLVSGEH---IG 128

Query: 154 AFAATEPGAGSDLLAMKTRAVKKGDKYVINGQKCFITNGSVADILTVWAYTDPSKGAKGM 213
           A A +EP AGSD+++MK  A  +GD Y++NG K +ITNG  A+   ++A TD S G++G+
Sbjct: 129 ALAMSEPNAGSDVISMKLTAKDEGDHYLLNGNKMWITNGPDANTYVIYAKTDTSAGSRGV 188

Query: 214 STFVVERGTPGLIYGHNEKKMGMRGCPNSELFFEDLEVPAENLVGEEGKGFAYLMGALSI 273
           + F+VER  PG        K+GMRG    EL F+D +VP EN++G  G G   LM  L  
Sbjct: 189 TAFIVERDAPGFSRHQKLDKLGMRGSNTCELVFQDCKVPKENVLGGVGNGAKVLMSGLDY 248

Query: 274 NRVFCASQAVGIAQGALERAMQHTREREQFGKPIAHLTPIQFMIADMATEVEAARLLVRK 333
            R+  +   +GI Q A++  + + RER+QFG+ I     +Q  +ADM T +  A+  V  
Sbjct: 249 ERLVLSGGPLGIMQAAMDVVVPYIRERKQFGQAIGEFELVQGKVADMYTWMNTAKSYVYM 308

Query: 334 ATTLLDAKDKRGPLIGGMAKTFASDTAMKVTTDAVQVMGGSGYMQEYQVERMMREAKLTQ 393
                D   +        A  ++++ A K+  DA+Q++GG+GY+ EY   R++R+AKL +
Sbjct: 309 VAMSADRGAETTRKDAAGAILYSAEMATKIALDAIQLLGGNGYINEYPTGRLLRDAKLYE 368

Query: 394 IYTGTNQITRMVTGRSL 410
           I  GT++I RM+ GR L
Sbjct: 369 IGAGTSEIRRMLIGREL 385


Lambda     K      H
   0.318    0.133    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 379
Number of extensions: 14
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 414
Length of database: 389
Length adjustment: 31
Effective length of query: 383
Effective length of database: 358
Effective search space:   137114
Effective search space used:   137114
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory