Align ring 1,2-phenylacetyl-CoA epoxidase PaaA subunit (EC 1.14.13.149) (characterized)
to candidate GFF2753 HP15_2697 phenylacetate-CoA oxygenase, PaaG subunit
Query= metacyc::MONOMER-15947 (330 letters) >FitnessBrowser__Marino:GFF2753 Length = 330 Score = 595 bits (1534), Expect = e-175 Identities = 284/330 (86%), Positives = 302/330 (91%) Query: 1 MYAQLVETGVKRVKALEEMAPEERAFQERIDAEIKIEAKNWMPDAYRQTLIRQISQHAHS 60 MYAQLVETG KR+K EEM+PEER FQE++DAE KIE KNWMP+ YR+TLIRQISQHAHS Sbjct: 1 MYAQLVETGAKRLKTKEEMSPEERDFQEKVDAETKIEPKNWMPEGYRKTLIRQISQHAHS 60 Query: 61 EIVGMLPEGNWVTRAPTLKRKLQLMAKIQDEAGHGLYLYSAMETLGADRDEEIAKLHSGK 120 E+VGMLPEGNWVTRAPTLKRKLQLMAKIQDEAGHGLYLYSAMETLGADRDEEI KLH GK Sbjct: 61 EVVGMLPEGNWVTRAPTLKRKLQLMAKIQDEAGHGLYLYSAMETLGADRDEEIEKLHQGK 120 Query: 121 AKYSSIFNYPTLNWADMGAVGWLVDGAAIVNQVVLQRTSYGPYSRAMIRICKEESFHQRQ 180 AKYSSIFNYPTLNWADMGAVGWLVDGAAIVNQVVLQRTSYGPYSRAMIRICKEESFHQRQ Sbjct: 121 AKYSSIFNYPTLNWADMGAVGWLVDGAAIVNQVVLQRTSYGPYSRAMIRICKEESFHQRQ 180 Query: 181 GYEILLTMMRHGTQAQKDMVQDAINRLWWPALMMFGPSDEHSPNSAQSMAWKIKRQSNDE 240 GY+ILL MMR GT+ QK MVQDAINRLWWPALMMFGP D+ SPNS QSMAWKIKR+SNDE Sbjct: 181 GYQILLDMMREGTEEQKAMVQDAINRLWWPALMMFGPHDDESPNSQQSMAWKIKRKSNDE 240 Query: 241 LRQRFIDQTVPQLELLGCTAPDPELKWNEERGHYDFGAIDWSEFYEVLKGNGPCNAERIA 300 LRQ FIDQTVPQLE LGCTAPDP+LKWNEE GHYDFG I+W EFY+VLKGNGPCN ERI Sbjct: 241 LRQMFIDQTVPQLEFLGCTAPDPDLKWNEETGHYDFGEINWQEFYDVLKGNGPCNRERIK 300 Query: 301 TRRNAIDNGAWVREAAVAHARKQQHKRDAA 330 TR+NAID GAWVREAAVA+A KQ+ + AA Sbjct: 301 TRKNAIDEGAWVREAAVAYAEKQKQRAQAA 330 Lambda K H 0.318 0.131 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 511 Number of extensions: 12 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 330 Length of database: 330 Length adjustment: 28 Effective length of query: 302 Effective length of database: 302 Effective search space: 91204 Effective search space used: 91204 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory