GapMind for catabolism of small carbon sources

 

Aligments for a candidate for HSERO_RS00895 in Marinobacter adhaerens HP15

Align ABC transporter ATP-binding protein (characterized, see rationale)
to candidate GFF3115 HP15_3058 leucine/isoleucine/valine transporter ATP-binding subunit

Query= uniprot:A0A165KC86
         (260 letters)



>lcl|FitnessBrowser__Marino:GFF3115 HP15_3058
           leucine/isoleucine/valine transporter ATP-binding
           subunit
          Length = 251

 Score =  217 bits (553), Expect = 2e-61
 Identities = 104/249 (41%), Positives = 166/249 (66%), Gaps = 1/249 (0%)

Query: 9   VLKVAGISKRFGGLQALSDVGITIKRGQVYGLIGPNGAGKTTFFNVITGLYTPDAGTFEL 68
           +L+V  +S RFGGL A+ +V + ++  ++  +IGPNGAGKTT FN ++G Y P  G    
Sbjct: 1   MLEVQNLSMRFGGLLAVDEVSLDVQEHEIVSIIGPNGAGKTTVFNCMSGFYKPTGGKILF 60

Query: 69  AGKPYEPTAVHEVAKAGIARTFQNIRLFAEMTALENVMVGRHIRTGSGLFGAVFRTKGFK 128
            GK  +    +++++ G+ RTFQ++RLF++MT +EN++V +H    + L   + +T  ++
Sbjct: 61  EGKEVQGKPDYKISRLGMVRTFQHVRLFSQMTVVENLLVAQHRHLNTNLISGLIQTPSYR 120

Query: 129 AEEAAIAKRAQELLDYVGIGKFADYKARTLSYGDQRRLEIARALATDPQLIALDEPAAGM 188
            +E     RA   LD VG+   A+ +A  L+YG QRRLEIAR + T+P+L+ LDEPAAG+
Sbjct: 121 KKEQKSLDRAAYWLDRVGLLDMANREAGNLAYGQQRRLEIARCMVTEPKLLMLDEPAAGL 180

Query: 189 NATEKVQLRELIDRIRND-NRTILLIEHDVKLVMGLCDRVTVLDYGKQIAEGNPAEVQKN 247
           N  E  +L +LI  ++ D N +++LIEHD+ LVM + DR+ V++ G+ +A G P E+++N
Sbjct: 181 NPAETKELNQLIISLKEDYNVSVVLIEHDMSLVMDISDRINVINQGRPLASGTPEEIRQN 240

Query: 248 EKVIEAYLG 256
           + VI+AYLG
Sbjct: 241 DDVIKAYLG 249


Lambda     K      H
   0.319    0.137    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 186
Number of extensions: 8
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 260
Length of database: 251
Length adjustment: 24
Effective length of query: 236
Effective length of database: 227
Effective search space:    53572
Effective search space used:    53572
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory