GapMind for catabolism of small carbon sources

 

propionate catabolism in Marinobacter adhaerens HP15

Best path

putP, prpE, prpC, acnD, prpF, acn, prpB

Also see fitness data for the top candidates

Rules

Overview: Propionate degradation in GapMind is based on MetaCyc pathways for the 2-methylcitrate cycle (link, link) and for propanoyl-CoA degradation (link, link).

24 steps (17 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
putP propionate transporter; proline:Na+ symporter HP15_2205
prpE propionyl-CoA synthetase HP15_811 HP15_2053
prpC 2-methylcitrate synthase HP15_1931 HP15_1516
acnD 2-methylcitrate dehydratase (2-methyl-trans-aconitate forming) HP15_1930 HP15_3433
prpF methylaconitate isomerase HP15_1929
acn (2R,3S)-2-methylcitrate dehydratase HP15_1930 HP15_2203
prpB 2-methylisocitrate lyase HP15_1932
Alternative steps:
dddA 3-hydroxypropionate dehydrogenase HP15_2656 HP15_2800
epi methylmalonyl-CoA epimerase
hpcD 3-hydroxypropionyl-CoA dehydratase HP15_12 HP15_2692
iolA malonate semialdehyde dehydrogenase (CoA-acylating) HP15_2655 HP15_906
lctP propionate permease
mcm-large methylmalonyl-CoA mutase, large (catalytic) subunit
mcm-small methylmalonyl-CoA mutase, small (adenosylcobamide-binding) subunit HP15_1792
mcmA methylmalonyl-CoA mutase, fused catalytic and adenosylcobamide-binding components
mctC propionate:H+ symporter
mctP propionate permease
pccA propionyl-CoA carboxylase, alpha subunit HP15_1002 HP15_4179
pccA1 propionyl-CoA carboxylase, biotin carboxyl carrier subunit HP15_3934 HP15_3222
pccA2 propionyl-CoA carboxylase, biotin carboxylase subunit HP15_3935
pccB propionyl-CoA carboxylase, beta subunit HP15_1004 HP15_2538
pco propanyl-CoA oxidase HP15_3936 HP15_2
prpD 2-methylcitrate dehydratase HP15_1928
SLC5A8 sodium-coupled monocarboxylate transporter

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory