GapMind for catabolism of small carbon sources

 

Aligments for a candidate for pccA1 in Marinobacter adhaerens HP15

Align acyl CoA carboxylase biotin carboxylase subunit (EC 2.1.3.15; EC 6.4.1.3; EC 6.3.4.14) (characterized)
to candidate GFF3994 HP15_3934 acetyl-CoA carboxylase biotin carboxylase subunit

Query= metacyc::MONOMER-13597
         (509 letters)



>lcl|FitnessBrowser__Marino:GFF3994 HP15_3934 acetyl-CoA carboxylase
           biotin carboxylase subunit
          Length = 473

 Score =  414 bits (1064), Expect = e-120
 Identities = 219/471 (46%), Positives = 307/471 (65%), Gaps = 7/471 (1%)

Query: 6   RVLVANRGEIATRVLKAIKEMGMTAIAVYSEADKYAVHTKYADEAYYIGKAPALDSYLNI 65
           ++L+ANRGEIA R+ +A  E+G+ ++A++SEAD+Y++H K ADEAY I K P L  YLN 
Sbjct: 5   KLLIANRGEIAVRIARACSELGIRSVAIHSEADEYSLHVKKADEAYQISKDP-LSGYLNP 63

Query: 66  EHIIDAAEKAHVDAIHPGYGFLSENAEFAEAVEKAGITFIGPSSEVMRKIKDKLDGKRLA 125
            HI++ A +   DA+HPGYGFLSENAE A   E+ GITF+GPS+  +  + DK   ++ A
Sbjct: 64  HHIVNMAVETGCDALHPGYGFLSENAELAAICEQRGITFVGPSANAISSMGDKTQARQTA 123

Query: 126 NMAGVPTAPGSDGPVTSIDEALKLAEKIGYPIMVKAASGGGGVGITRVDNQDQLMDVWER 185
             AGVP  PGS+G +  +++A+  A  IGYP+M+KA SGGGG GI R DN+ +L   +ER
Sbjct: 124 LAAGVPVTPGSEGNLADVEDAVVQAADIGYPVMLKATSGGGGRGIRRCDNEKELRQNFER 183

Query: 186 NKRLAYQAFGKADLFIEKYAVNPRHIEFQLIGDKYGNYVVAWERECTIQRRNQKLIEEAP 245
               A +AFG A++F+EK  + PRHIE Q++ D +GN V  +ER+C+IQRRNQKLIE AP
Sbjct: 184 VISEATKAFGSAEVFLEKCIIEPRHIEVQILADTHGNVVHLYERDCSIQRRNQKLIELAP 243

Query: 246 SPALKMEERESMFEPIIKFGKLINYFTLGTFETAFSDVSRDFYFLELNKRLQVEHPTTEL 305
           SP L+  +RE + +   +  K   Y   GT E    D    FYF+E+N R+QVEH  TE 
Sbjct: 244 SPQLEESQREYIGDLAKRVAKQCGYVNAGTVEFLL-DHDGSFYFMEMNTRVQVEHTITEE 302

Query: 306 IFRIDLVKLQIKLAAGEHLPFSQEDLNKRVRGTAIEYRINAEDALNNFTGSSGFVTYYRE 365
           I  +D++K QI++AAGE L   QED++   RG A ++RINAED  N F  S G ++ Y  
Sbjct: 303 ITGVDIIKAQIRIAAGEPLGLKQEDIS--YRGFAAQFRINAEDPKNGFLPSFGRISRYYS 360

Query: 366 PTGPGVRVDSGIESGSYVPPYYDSLVSKLIVYGESREYAIQAGIRALADYKIGGIKTTIE 425
             GPGVR D+ + +G  +PPYYDS+ +KLIV+    +  I    RAL D  I G++TTI 
Sbjct: 361 AGGPGVRTDANMYTGYEIPPYYDSMCAKLIVWAMDWDELIARSRRALGDMGIYGVQTTIP 420

Query: 426 LYKWIMQDPDFQEGKFSTSYISQKTDQFVKYLRE--QEEIKAAIAAEIQSR 474
            YK I++ PDFQ   F+T ++ ++  Q ++Y  +   E I  AIAA I ++
Sbjct: 421 YYKQILEHPDFQAADFNTGFV-ERNPQLLEYSSKTRPESIATAIAAAIAAQ 470


Lambda     K      H
   0.317    0.135    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 595
Number of extensions: 27
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 509
Length of database: 473
Length adjustment: 34
Effective length of query: 475
Effective length of database: 439
Effective search space:   208525
Effective search space used:   208525
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory