Alignments for a candidate for potA in Marinobacter adhaerens HP15

GapMind for catabolism of small carbon sources

Alignments for a candidate for potA in Marinobacter adhaerens HP15

Align spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 (characterized)
to candidate GFF4122 HP15_4062 spermidine/putrescine ABC transporter, ATP-binding protein

Query= CharProtDB::CH_024626
         (378 letters)



>FitnessBrowser__Marino:GFF4122
          Length = 373

 Score =  423 bits (1088), Expect = e-123
 Identities = 219/360 (60%), Positives = 275/360 (76%), Gaps = 5/360 (1%)

Query: 17  LVQLAGIRKCFDGKEVIPQLDLTINNGEFLTLLGPSGCGKTTVLRLIAGLETVDSGRIML 76
           L+ L+ + K F GK V+  LDL I +GEF+TLLGPSGCGKTT+LRL+AG E  D G I L
Sbjct: 5   LLSLSNLSKQFGGKTVLDGLDLEIYDGEFITLLGPSGCGKTTLLRLMAGFEHPDEGTITL 64

Query: 77  DNEDITHVPAENRYVNTVFQSYALFPHMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQ 136
             E++TH   ENR +NTVFQ YALFPHM+VF+NVA+GL+M+K P  EI  RV EAL MVQ
Sbjct: 65  AGENLTHTAPENRPLNTVFQHYALFPHMSVFDNVAYGLKMEKRPKDEIRQRVDEALAMVQ 124

Query: 137 LETFAQRKPHQLSGGQQQRVAIARAVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRK 196
           L+ FA+RKPHQLSGGQQQRVAIARAVV +PRLLLLDE LSALDYKLR+ MQ ELK LQR+
Sbjct: 125 LQDFARRKPHQLSGGQQQRVAIARAVVKRPRLLLLDEPLSALDYKLRRTMQVELKRLQRE 184

Query: 197 LGITFVFVTHDQEEALTMSDRIVVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNA 256
           LGITFVFVTHDQEEAL+MSDR+VV++DG ++Q GTPRE+YE P NLF A F+GE N F  
Sbjct: 185 LGITFVFVTHDQEEALSMSDRVVVLKDGLVQQLGTPREVYERPANLFTARFVGETNFFPG 244

Query: 257 TVIERLDEQRVRANVEGRECNI-YVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIG 315
           TV E + +  ++ +V G +  +   +F V+  Q LHVLLRPED+RV E +D+N   G+ G
Sbjct: 245 TV-ESVQDGSIKVDVFGLKRTLRRPDFPVQAEQSLHVLLRPEDIRVLEPDDEN---GVAG 300

Query: 316 YVRERNYKGMTLESVVELENGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADE 375
            + ERNYKG TL+SV+ L +G  V+ SEFF+EDDP FD+ L + + ++WV+ WE +L +E
Sbjct: 301 KIVERNYKGSTLDSVIHLADGTEVLASEFFDEDDPAFDYRLGEPVKVSWVDGWEWLLPEE 360


Lambda     K      H
   0.319    0.136    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 430
Number of extensions: 18
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 378
Length of database: 373
Length adjustment: 30
Effective length of query: 348
Effective length of database: 343
Effective search space:   119364
Effective search space used:   119364
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources