GapMind for catabolism of small carbon sources

 

pyruvate catabolism in Marinobacter adhaerens HP15

Best path

yjcH, actP

Also see fitness data for the top candidates

Rules

Overview: Pyruvate is a central metabolite, so GapMind represents transport only.

13 steps (5 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
yjcH putative small subunit of pyruvate transporter (yjcH-like) HP15_1252
actP large subunit of pyruvate transporter (actP-like) HP15_1251
Alternative steps:
btsT pyruvate symporter BtsT
cstA large subunit of pyruvate transporter (CstA)
dctM pyruvate TRAP transporter, large permease component HP15_3249 HP15_3797
dctP pyruvate TRAP transporter, substrate-binding component HP15_3247 HP15_4061
dctQ pyruvate TRAP transporter, small permease component HP15_3796 HP15_3248
JEN1 pyruvate symporter JEN1
MCT4 pyruvate symporter MCT4 (SLC16A3)
mctC pyruvate symporter MctC
mctP pyruvate permease MctP
SLC5A8 sodium-coupled pyruvate transporter
ybdD small subunit of pyruvate transporter (YbdD)

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory