Align Branched chain amino acid ABC transporter substrate-binding protein (characterized, see rationale)
to candidate GFF3112 HP15_3055 high-affinity leucine-specific leucine-specific-binding periplasmic protein of high-affinity branched-chain amino acid ABC transporter transport system periplasmic binding protein
Query= uniprot:A0A165KTD4 (375 letters) >lcl|FitnessBrowser__Marino:GFF3112 HP15_3055 high-affinity leucine-specific leucine-specific-binding periplasmic protein of high-affinity branched-chain amino acid ABC transporter transport system periplasmic binding protein Length = 370 Score = 172 bits (435), Expect = 2e-47 Identities = 118/381 (30%), Positives = 193/381 (50%), Gaps = 24/381 (6%) Query: 3 LKLKLTVVAAIAAAAGVASAQEQVVKIGHVAPVSGAQAHYGKDNENGARMAIEELNAQGV 62 +K +T V+ A G A ++ +IG P++G A YG +GARMAIE++NA G Sbjct: 5 VKKLVTAVSTSVALMGAGHAAAEI-QIGIAGPMTGPVAQYGDMQFSGARMAIEQINANGG 63 Query: 63 TIGGKKIKFELVAEDDAADPKQGTAAAQKLCDAKVAGVVGHLNSGTTIPASKVYNDCGIP 122 +G + + E DD DPKQ A L + V V+GHL S +T PAS +Y D GI Sbjct: 64 VMGEELVAVEY---DDVCDPKQAVTVANSLVNDGVRFVIGHLCSSSTQPASDIYEDEGIL 120 Query: 123 HVTGAATNPNLTKPGYKTTFRIIANDNALGAGLAFYAVDTLKLKTVAIIDDRTAYGQGVA 182 VT A+T+P +T+ GY+ FR I D+ G A Y + + + VAI+ D+ YG+G+A Sbjct: 121 MVTPASTSPEITERGYELVFRTIGLDSMQGPVAARY-IASQNPERVAIVHDKQQYGEGIA 179 Query: 183 DVFKKTATAKGMKVVDEQFTTDKATDFMAILTAIKAKNPDAIFYGGMDPQGGPMLRQMEQ 242 + T G+++ + T DF +++T +K + D ++YGG P+ G +LRQ Sbjct: 180 TAVRDTLKDAGVEIAMFEGITAGDKDFSSLVTKLKQADVDYVYYGGYHPELGLILRQANS 239 Query: 243 LGMGNVKYFGGDGICTSEIAKLAAGAKTLGNVICAEGGSSLAKMPGGTAWKAK------- 295 + + ++ G +G+ +I +A A AEG L +P KA+ Sbjct: 240 ADL-DARFMGPEGVGNKDINTIAGEA--------AEG--LLVTLPPAFDQKAENQALVKA 288 Query: 296 YDAKYPNQFQVYSPYTYDATFLIVDAMKRANSVDPKVYTPELAKSSFKGVTSTIAFEPNG 355 ++ K + + +Y A L+ + ++ A S DP L + +F+ T+ ++ G Sbjct: 289 FEDKGEDPSGPFVLTSYTAVQLVAEGIEAAGSTDPFDVAAALREGTFQTPIGTVEYDKAG 348 Query: 356 EMKNPAITLYV-YKDGKKTPL 375 +MK+ +Y + DG KTP+ Sbjct: 349 DMKSFEFVVYEWHSDGSKTPV 369 Lambda K H 0.315 0.131 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 379 Number of extensions: 17 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 375 Length of database: 370 Length adjustment: 30 Effective length of query: 345 Effective length of database: 340 Effective search space: 117300 Effective search space used: 117300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory