Align propionyl-CoA carboxylase α subunit (EC 6.4.1.3) (characterized)
to candidate GFF1025 HP15_1004 3-methylcrotonyl CoA carboxylase, beta subunit
Query= metacyc::MONOMER-17283 (535 letters) >FitnessBrowser__Marino:GFF1025 Length = 535 Score = 745 bits (1924), Expect = 0.0 Identities = 363/535 (67%), Positives = 428/535 (80%) Query: 1 MSIIHSHIQPNSPDFQANFAYHQSLAADLRERLAQIRQGGGAEQRRRHEERGKLFVRDRI 60 M+I+ S I P S +FQAN ADLR++++ I+QGGG + RH RGKL R+RI Sbjct: 1 MTILQSKINPRSEEFQANQESMAQAVADLRDKVSTIQQGGGPSYQERHIARGKLLPRERI 60 Query: 61 DTLIDPDSSFLEIGALAAYNVYDEEVPAAGIVCGIGRVAGRPVMIIANDATVKGGTYFPL 120 + L+D S FLEIG AAYNVYDEEVPAAG++ G+GRV+G MIIANDATVKGG+Y+PL Sbjct: 61 NRLLDDGSPFLEIGQFAAYNVYDEEVPAAGVIAGVGRVSGTECMIIANDATVKGGSYYPL 120 Query: 121 TVKKHLRAQEIARENRLPCIYLVDSGGAYLPLQSEVFPDRDHFGRIFYNQAQMSAEGIPQ 180 TVKKHLRAQEIA +NRLPCIYLVDSGGA LP Q EVFPDR+HFGRIFYNQA+MSA+ IPQ Sbjct: 121 TVKKHLRAQEIALQNRLPCIYLVDSGGANLPRQDEVFPDREHFGRIFYNQARMSADDIPQ 180 Query: 181 IACVMGSCTAGGAYVPAMSDEVVIVKGNGTIFLGGPPLVKAATGEEVTAEELGGADVHTR 240 IA VMG CTAGGAYVPAM+DE +IV+ GTIFL GPPLVKAATGE VTAE+LGGADVH + Sbjct: 181 IAVVMGLCTAGGAYVPAMADESIIVRNQGTIFLAGPPLVKAATGEVVTAEDLGGADVHCK 240 Query: 241 ISGVADYFANDDREALAIVRDIVAHLGPRQRANWELRDPEPPRYDPREIYGILPRDFRQS 300 ISGVAD++A +D AL I R V++L R+ + E+R P+ P YD EIYGI+ D R+ Sbjct: 241 ISGVADHYAENDAHALEIARRSVSNLNRRKPTDVEIRKPKAPLYDAEEIYGIVGTDLRKQ 300 Query: 301 YDVREVIARIVDGSRLHEFKTRYGTTLVCGFAHIEGFPVGILANNGILFSESALKGAHFI 360 +DVR+VIAR VDGS EFK YG TLV GFAHI G+PVGI+ANNGILFSE+A KGAHF+ Sbjct: 301 FDVRDVIARTVDGSEFDEFKRYYGQTLVTGFAHIHGYPVGIIANNGILFSEAAQKGAHFV 360 Query: 361 ELCCARNIPLVFLQNITGFMVGKQYENGGIAKDGAKLVTAVSCANVPKFTVIIGGSFGAG 420 ELCC RNIPL+FLQNITGFMVG++YE+ GIAK GAK+V AV+CANVPK TV+IGGSFGAG Sbjct: 361 ELCCQRNIPLLFLQNITGFMVGQKYESEGIAKHGAKMVMAVACANVPKITVLIGGSFGAG 420 Query: 421 NYGMCGRAYQPRQLWMWPNARISVMGGTQAANVLLTIRRDNLRARGQDMTPEEQERFMAP 480 NYGMCGRAY P LWMWPNARISVMGG QAA V+ T+RR+ + +GQ+ + EE+ F P Sbjct: 421 NYGMCGRAYSPDFLWMWPNARISVMGGEQAAGVMATVRREGMERKGQEWSAEEEAEFKKP 480 Query: 481 ILAKYEQEGHPYYASARLWDDGVIDPVETRRVLALGLAAAAEAPVQPTRFGVFRM 535 I+ YE +GHPYYASARLWDDGVIDP +TR V+AL L+A P +PTRFGVFRM Sbjct: 481 IIDTYEHQGHPYYASARLWDDGVIDPAQTREVVALSLSATLNRPAKPTRFGVFRM 535 Lambda K H 0.322 0.139 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 966 Number of extensions: 46 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 535 Length of database: 535 Length adjustment: 35 Effective length of query: 500 Effective length of database: 500 Effective search space: 250000 Effective search space used: 250000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory