GapMind for catabolism of small carbon sources

 

Alignments for a candidate for aglG in Marinobacter adhaerens HP15

Align ABC transporter for D-Maltose and D-Trehalose, permease component 2 (characterized)
to candidate GFF2207 HP15_2161 sugar ABC transporter, permease protein

Query= reanno::Smeli:SMc03063
         (380 letters)



>FitnessBrowser__Marino:GFF2207
          Length = 269

 Score =  127 bits (319), Expect = 4e-34
 Identities = 73/232 (31%), Positives = 124/232 (53%), Gaps = 7/232 (3%)

Query: 146 PPRFTLDNYAEVLSAAGIGRSFLNSLTVAVPSTVIPILIAAFAAYALAWMPFPGRAVLLA 205
           P  FTLDNYA + +       ++NS+     + VI +L+A  AAYA +   F G   L  
Sbjct: 45  PQNFTLDNYAVIFTDPSWYNGYINSMIYVSINVVITLLVALPAAYAFSRYKFLGDKHLFF 104

Query: 206 VVVGLLVVPLQMSLIPLLQLYNGVGAFFGVSAKTYMGIWLAHTGFGLPLAIYLLRNYMAG 265
            ++   + P  + L+P  QLY+ +G F      T++ + LAH  F +PLA+++L  +M+G
Sbjct: 105 WLLSNRMAPPAVFLLPFFQLYSSIGLF-----DTHIAVALAHCLFNVPLAVWILEGFMSG 159

Query: 266 LPREIMESARVDGASDFDIFVKIILPLSFPALASFAIFQFLWTWNDLLVAIVFLGAGDDK 325
           +PRE  E A +DG S    FVK+ LP+    +   A F F+++W +LL+A        D 
Sbjct: 160 VPREYDEMAYIDGYSFPKFFVKVFLPMIRSGIGVTAFFAFMFSWVELLLARTLTSV--DA 217

Query: 326 LVLTGRLVNLLGSRGGNWEILTASAFITIVVPLIVFFALQRYLVRGLLAGSV 377
             +   +   +G+ G +W +L A+  +TI+  L+V + ++ ++ +G   G V
Sbjct: 218 QPIGAIMTRTIGASGIDWGVLAAAGSLTIIPGLLVIWFVRNHVAKGFALGRV 269


Lambda     K      H
   0.324    0.139    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 234
Number of extensions: 15
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 380
Length of database: 269
Length adjustment: 28
Effective length of query: 352
Effective length of database: 241
Effective search space:    84832
Effective search space used:    84832
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory