Align Citrate/2-methylcitrate dehydratase; EC 4.2.1.- (characterized)
to candidate GFF1971 HP15_1928 2-methylcitrate dehydratase PrpD
Query= SwissProt::P45859 (472 letters) >FitnessBrowser__Marino:GFF1971 Length = 494 Score = 605 bits (1559), Expect = e-177 Identities = 294/481 (61%), Positives = 378/481 (78%), Gaps = 13/481 (2%) Query: 2 PKTDRVIEEITDYVLEKEITSAEAYTTAGHVLLDTLGCGILALRYPECTKLLGPIVPGTT 61 P D V+++I DYVL EITS EA+ TA + L+DTLGC +LALR+PECTK LGPIV GT Sbjct: 11 PDYDEVLQKIADYVLTYEITSEEAWNTARYCLMDTLGCALLALRFPECTKHLGPIVEGTM 70 Query: 62 VPNGSKVPGTSYVLDPVRAAFNIGCMIRWLDYNDTWLAAEWGHPSDNLGGILAAADYVSR 121 VP+G++VPGT Y LDPV+AA++IGC +RWLDYNDTWLAAEWGHPSDNLG ILA AD++S+ Sbjct: 71 VPHGARVPGTPYRLDPVKAAWDIGCNVRWLDYNDTWLAAEWGHPSDNLGAILAVADHLSQ 130 Query: 122 VRLSEGKEPLTVRDVLEMMIKAHEIQGVLALENSLNRVGLDHVLFVKVATTAVAAKLLGG 181 R++EGKEPLT+R VLE MI AHEIQGVLALENS NRVGLDHV+ VKVA+TAVA++L+G Sbjct: 131 KRVAEGKEPLTMRTVLEAMIMAHEIQGVLALENSFNRVGLDHVVLVKVASTAVASRLMGA 190 Query: 182 GREEIKNALSNAWIDNAALRTYRHSPNTGSRKSWAAGDATSRGVHLALMSLKGEMGYPTA 241 RE++ +ALS+AW+D +LRTYRH+PN GSRKSWAAGDATSR V LA ++++GEMG P Sbjct: 191 DREQLLSALSHAWVDGQSLRTYRHAPNAGSRKSWAAGDATSRAVRLADIAMRGEMGVPGV 250 Query: 242 LSAPGWGFQDVLF------------NKKEIKLARPLDAYVMENVLFKVSYPAEFHAQTAA 289 L+AP WGF DVLF +K++ L + +YVMEN+LFK+S+PAEFHAQTAA Sbjct: 251 LTAPQWGFYDVLFSKTNKDQKLKPEDKRQFSLPQEFGSYVMENILFKISFPAEFHAQTAA 310 Query: 290 ESAVILHPQVKNRIDEIDRVVIRTHESAIRIIDKKGPLHNPADRDHCLQYITAIGLLFGD 349 E+AVIL+P+VK+R++EI+++VI THESAIRII K G L N ADRDHCLQY+ A+ L FG Sbjct: 311 EAAVILYPEVKDRLNEINKIVITTHESAIRIISKVGKLANAADRDHCLQYMAAVPLAFGS 370 Query: 350 ITAQHYEAETAN-DPRIDKLRDKMEVTENKTYTEDYLKPDKRSISNAVQVHFKDGTSTEM 408 +TA+HYE +P ID+LRDKMEV E++ YT +YL+P+KRSI+NA+QV F DG++T+ Sbjct: 371 LTAEHYEDSFHRANPIIDELRDKMEVVEDERYTREYLEPEKRSIANAIQVFFNDGSNTDK 430 Query: 409 VECEFPLGHRFRREEAVPKLLEKFSDNLKTHFPDKQHKHIYERCTSYETLQTMRVNEFVD 468 + E+P+GHR RR+E +P L EKF NLKT P K+ + + C E L+ V+EF++ Sbjct: 431 IAVEYPIGHRRRRKEGMPLLEEKFLSNLKTRLPSKRCDTVLKMCQDPEKLENTPVHEFMN 490 Query: 469 M 469 + Sbjct: 491 L 491 Lambda K H 0.319 0.135 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 676 Number of extensions: 23 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 472 Length of database: 494 Length adjustment: 34 Effective length of query: 438 Effective length of database: 460 Effective search space: 201480 Effective search space used: 201480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 52 (24.6 bits)
Align candidate GFF1971 HP15_1928 (2-methylcitrate dehydratase PrpD)
to HMM TIGR02330 (prpD: 2-methylcitrate dehydratase (EC 4.2.1.79))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR02330.hmm # target sequence database: /tmp/gapView.9573.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR02330 [M=468] Accession: TIGR02330 Description: prpD: 2-methylcitrate dehydratase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 6.4e-264 861.7 0.0 7.2e-264 861.5 0.0 1.0 1 lcl|FitnessBrowser__Marino:GFF1971 HP15_1928 2-methylcitrate dehydr Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__Marino:GFF1971 HP15_1928 2-methylcitrate dehydratase PrpD # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 861.5 0.0 7.2e-264 7.2e-264 1 468 [] 14 494 .] 14 494 .] 1.00 Alignments for each domain: == domain 1 score: 861.5 bits; conditional E-value: 7.2e-264 TIGR02330 1 davlediadyvleyeidskeaydtaryvlldtlgcgllaleypectkllgpvvegtlvpngarvpgtsyqldpvk 75 d+vl++iadyvl yei+s+ea++tary+l+dtlgc llal++pectk+lgp+vegt+vp+garvpgt y+ldpvk lcl|FitnessBrowser__Marino:GFF1971 14 DEVLQKIADYVLTYEITSEEAWNTARYCLMDTLGCALLALRFPECTKHLGPIVEGTMVPHGARVPGTPYRLDPVK 88 89************************************************************************* PP TIGR02330 76 aafnigalvrwldyndtwlaaewghpsdnlggilavadylsrkriaegkeplkvkevleamikaheiqgvlalen 150 aa++ig+ vrwldyndtwlaaewghpsdnlg+ilavad+ls+kr+aegkepl+++ vleami+aheiqgvlalen lcl|FitnessBrowser__Marino:GFF1971 89 AAWDIGCNVRWLDYNDTWLAAEWGHPSDNLGAILAVADHLSQKRVAEGKEPLTMRTVLEAMIMAHEIQGVLALEN 163 *************************************************************************** PP TIGR02330 151 sfnrvgldhvllvkvastavvakllgatreeilnalshafvdgqalrtyrhapntgsrkswaagdatsrgvrlal 225 sfnrvgldhv+lvkvastav+++l+ga+re++l+alsha+vdgq+lrtyrhapn+gsrkswaagdatsr+vrla+ lcl|FitnessBrowser__Marino:GFF1971 164 SFNRVGLDHVVLVKVASTAVASRLMGADREQLLSALSHAWVDGQSLRTYRHAPNAGSRKSWAAGDATSRAVRLAD 238 *************************************************************************** PP TIGR02330 226 ialkgemgypsalsapvwgfedvlfkke............klklareygsyvmenvlfkisfpaefhaqtaveaa 288 ia++gemg+p++l+ap+wgf+dvlf+k+ +++l++e+gsyvmen+lfkisfpaefhaqta+eaa lcl|FitnessBrowser__Marino:GFF1971 239 IAMRGEMGVPGVLTAPQWGFYDVLFSKTnkdqklkpedkrQFSLPQEFGSYVMENILFKISFPAEFHAQTAAEAA 313 **************************999********************************************** PP TIGR02330 289 vklheevkerldeierivitthesairiidkkgplanpadrdhclqylvavpllfgdlvaedyeda.vaadprid 362 v l++evk+rl+ei++ivitthesairii+k+g lan adrdhclqy++avpl fg l+ae+yed+ + a+p id lcl|FitnessBrowser__Marino:GFF1971 314 VILYPEVKDRLNEINKIVITTHESAIRIISKVGKLANAADRDHCLQYMAAVPLAFGSLTAEHYEDSfHRANPIID 388 ******************************************************************8999***** PP TIGR02330 363 elreklevvedkrysreyleadkrsianavevffkdgskteeveveyplghrrrrdegipklvdkfkanlatkfs 437 elr+k+evved+ry+reyle++krsiana++vff+dgs+t++++veyp+ghrrrr+eg+p+l +kf nl t+++ lcl|FitnessBrowser__Marino:GFF1971 389 ELRDKMEVVEDERYTREYLEPEKRSIANAIQVFFNDGSNTDKIAVEYPIGHRRRRKEGMPLLEEKFLSNLKTRLP 463 *************************************************************************** PP TIGR02330 438 skkqerilelcldqakleatpvnefldlfvi 468 sk+++ +l++c d +kle+tpv ef++l++i lcl|FitnessBrowser__Marino:GFF1971 464 SKRCDTVLKMCQDPEKLENTPVHEFMNLLLI 494 ****************************986 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (468 nodes) Target sequences: 1 (494 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.02 # Mc/sec: 8.50 // [ok]
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory