GapMind for catabolism of small carbon sources

 

Alignments for a candidate for aldox-small in Marinobacter adhaerens HP15

Align glycolaldehyde oxidoreductase small subunit (characterized)
to candidate GFF1287 HP15_1257 (2Fe-2S)-binding domain protein

Query= metacyc::MONOMER-18073
         (163 letters)



>FitnessBrowser__Marino:GFF1287
          Length = 161

 Score =  127 bits (319), Expect = 9e-35
 Identities = 65/143 (45%), Positives = 84/143 (58%)

Query: 12  IKVKVNGVLYERYVSPRILLVDFLREELGLTGTKIGCDTTTCGACTVLLNGKSVKSCTLF 71
           + + VNG      +     L+  LR+ELGL GTK GC    CGACTV LNG+ V+SC+  
Sbjct: 3   VSLTVNGDERSLNIEGDTPLLWVLRDELGLKGTKFGCGAGLCGACTVHLNGQPVRSCSTP 62

Query: 72  AVQADGAEITTIEGLSVDSKLHPIQEAFKENFALQCGFCTPGMIMQAYFLLKENPNPSEE 131
              A G ++ TIEGL    +LH +QEA+ E+   QCG+C  G IM A  LL  NP PS E
Sbjct: 63  VEMAAGGDVVTIEGLGEGRRLHALQEAWIEHGVPQCGYCQSGQIMSAAHLLASNPAPSRE 122

Query: 132 EVRDGLHGNICRCTGYQNIVKAV 154
           E+   + GN+CRC  Y  I+ AV
Sbjct: 123 EIVAAMTGNLCRCGTYSRIISAV 145


Lambda     K      H
   0.322    0.138    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 99
Number of extensions: 4
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 163
Length of database: 161
Length adjustment: 18
Effective length of query: 145
Effective length of database: 143
Effective search space:    20735
Effective search space used:    20735
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 43 (21.2 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory