Alignments for a candidate for lctP in Desulfovibrio vulgaris Miyazaki F

GapMind for catabolism of small carbon sources

Alignments for a candidate for lctP in Desulfovibrio vulgaris Miyazaki F

Align Glycolate permease, GlcA or YghK (substrates: L-lactate, D-lactate and glycolate) (characterized)
to candidate 8501398 DvMF_2129 L-lactate transport (RefSeq)

Query= TCDB::Q46839
         (560 letters)



>FitnessBrowser__Miya:8501398
          Length = 551

 Score =  542 bits (1396), Expect = e-158
 Identities = 282/561 (50%), Positives = 373/561 (66%), Gaps = 15/561 (2%)

Query: 2   VTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGAITLILSILIAIFAFKMP 61
           + W Q Y P+  + LSALVA +P+   F  LAV R KGH A A+    ++L+AI  + MP
Sbjct: 1   MNWVQNYDPLSNILLSALVAAVPLYVLFYMLAVRRAKGHFAAALGTTAAVLLAILVWGMP 60

Query: 62  IDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDIIRSSVISITDDQRLQVLLI 121
           +++A  A  YG  YGL+PI WI++ AV++Y +TV SG+F+II+ S+  +TDD+RLQ + I
Sbjct: 61  VNLAVNATLYGAAYGLFPIVWIVITAVWIYNMTVESGEFEIIKDSLARLTDDRRLQAIFI 120

Query: 122 GFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLCLIANTAPVAFGALGVPILVAG 181
            F+FG+ +EG AGFG PVAIT A+LVGLGF PLYAAG+CLIANTAPVAFGA+G+P++VAG
Sbjct: 121 AFAFGSFIEGTAGFGTPVAITAAMLVGLGFNPLYAAGICLIANTAPVAFGAIGIPVIVAG 180

Query: 182 QVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFT 241
           QV+G+D  HI  + GRQLPFLS++VP WL   M G+K   E  PA +VAG  FA +Q+  
Sbjct: 181 QVSGLDTMHISQIVGRQLPFLSIIVPLWLCVTMCGFKRSLEVLPAVIVAGVCFAGSQYAF 240

Query: 242 SNYIGPELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSE 301
           SN+ GP LPDI SA+++I+ L L L+VW+P  T               ++  S    PS 
Sbjct: 241 SNFHGPTLPDIMSAIITIIGLVLLLRVWKPATT-----------WHFPDETPSSLTGPSP 289

Query: 302 YSLGQIIRAWSPFLILTVLVTIWTMKPFKALF--APGGAFYSLVI-NFQIPHLHQQVLKA 358
           YS G+IIRAW P++IL ++V  W +   K     A G  F  L    F  P LH  + K 
Sbjct: 290 YSFGEIIRAWMPYIILAIMVFFWGLPEIKPYLDGALGAGFTPLAAKGFAWPGLHNAIEKT 349

Query: 359 APIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISIFIL-GVGIKKGIGVFAETLISLKWPI 417
           APIVA   P  A+FK + +SA GTAI    + ++ ++   G  + I     T+  L++PI
Sbjct: 350 APIVAANAPYGAMFKLNIISAAGTAILFTGLAAVPMMPKYGYGQAIPCLFRTMHQLRFPI 409

Query: 418 LSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGS 477
           L+I M+L  A + NYSGMS+T+ L    TG +FPFFSP LGWLGVFLTGSDTSSNALF  
Sbjct: 410 LTIAMILGLAQIMNYSGMSSTMGLAFTKTGWLFPFFSPLLGWLGVFLTGSDTSSNALFSG 469

Query: 478 LQSTTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL 537
           LQ TTA  + +   L VAAN+SGGVTGKMISPQSI+VA AAT MVG+E +LFR+T+ HS+
Sbjct: 470 LQRTTATSVGMDPHLAVAANSSGGVTGKMISPQSISVATAATKMVGQEGQLFRFTLWHSV 529

Query: 538 IFASVIGIITLLQAYVFTGML 558
              + I ++T+LQAY    ML
Sbjct: 530 AMTAFICVLTMLQAYPLKWML 550


Lambda     K      H
   0.327    0.141    0.424 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 904
Number of extensions: 35
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 560
Length of database: 551
Length adjustment: 36
Effective length of query: 524
Effective length of database: 515
Effective search space:   269860
Effective search space used:   269860
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources