GapMind for catabolism of small carbon sources

 

L-lactate catabolism in Desulfovibrio vulgaris Miyazaki F

Best path

lctP, lutA, lutB, lutC

Also see fitness data for the top candidates

Rules

Overview: L-lactate degradation in GapMind is based on L-lactate dehydrogenases or oxidases.

19 steps (14 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
lctP L-lactate:H+ symporter LctP or LidP DvMF_2129 DvMF_1861
lutA L-lactate dehydrogenase, LutA subunit DvMF_0397 DvMF_1868
lutB L-lactate dehydrogenase, LutB subunit DvMF_0396 DvMF_1868
lutC L-lactate dehydrogenase, LutC subunit DvMF_0395
Alternative steps:
ackA acetate kinase DvMF_2756 DvMF_1865
acs acetyl-CoA synthetase, AMP-forming DvMF_1775 DvMF_2783
DVU3032 L-lactate dehydrogenase, LutC-like component DvMF_1867 DvMF_0395
DVU3033 L-lactate dehydrogenase, fused LutA/LutB components DvMF_1868 DvMF_0396
L-LDH L-lactate dehydrogenase
larD D,L-lactic acid transporter LarD DvMF_0810
lctO L-lactate oxidase or 2-monooxygenase
lldE L-lactate dehydrogenase, LldE subunit DvMF_0397 DvMF_1868
lldF L-lactate dehydrogenase, LldF subunit DvMF_0396 DvMF_1868
lldG L-lactate dehydrogenase, LldG subunit DvMF_1867
mctP D,L-lactic acid transporter MctP
pta phosphate acetyltransferase DvMF_1864 DvMF_2625
SfMCT L-lactate transporter SfMCT
Shew_2731 L-lactate:Na+ symporter, large component DvMF_0315
Shew_2732 L-lactate:Na+ symporter, small component

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the preprint on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory