Align Probable 2-ketoarginine decarboxylase AruI; 2-oxo-5-guanidinopentanoate decarboxylase; 5-guanidino-2-oxopentanoate decarboxylase; EC 4.1.1.75 (characterized)
to candidate 8499376 DvMF_0153 acetolactate synthase, large subunit, biosynthetic type (RefSeq)
Query= SwissProt::Q9HUI8 (559 letters) >FitnessBrowser__Miya:8499376 Length = 562 Score = 241 bits (614), Expect = 7e-68 Identities = 176/541 (32%), Positives = 254/541 (46%), Gaps = 20/541 (3%) Query: 30 LTAGQALVRLLANYGVDTVFGIPGVHTLELYRGLPGSGIRHVLTRHEQGAGFMADGYARV 89 L + L+ L GVD +FG PG +++Y LP IRH+L RHEQ A ADG+AR Sbjct: 3 LNGARILLECLKREGVDVLFGYPGGAVIDIYDELPRHDIRHILVRHEQAAVHAADGFARA 62 Query: 90 SGKPGVCFVITGPGVTNVATAIGQAYADSVPLLVISSVNHSASLGKGWGCLHETQDQRAM 149 SGK GVC V +GPG TN T I AYADS+P++V++ + +G + D + Sbjct: 63 SGKVGVCLVTSGPGATNTVTGIATAYADSIPMVVLTGQVPTPLIGND---AFQEVDIVGI 119 Query: 150 TAPITAFSALALSPEQLPELIARAYAVFDSERPRPVHISIPLDVLAAPVAHDWSAAVARR 209 T P T + L L ++ +A+ + S RP PV + +P DV+ A W V R Sbjct: 120 TRPCTKHNYLVKDVRDLARVLRQAFYLARSGRPGPVLVDLPKDVMQAKTEFVWPEDVKLR 179 Query: 210 PGRGV--PCSEALRAAAERLAAARRPMLIAGGGALA--AGEALAALSERLAAPLFTSVAG 265 P LR A E L A+RP+ AGGG + A E L L+ L+ P+ +++ G Sbjct: 180 SYNPTYRPNLNQLRRAVEALIEAKRPLFFAGGGVIMSDASEELGWLARTLSIPVTSTLMG 239 Query: 266 KGLLPPDAPLNAG--ASLCVAPGWEMIAEADLVLAVGTEMADTDFWRERLPLSG-ELIRV 322 G P D PL G G + + AD ++AVG D R SG +I + Sbjct: 240 LGAFPGDDPLWLGMIGMHGTYAGNKAVNNADCIVAVGARFDDRVTGRLSAFASGARIIHI 299 Query: 323 DIDPRKFNDFYPSAVALRGDARQTL----EALLVRLPQE--ARDSAPAAARVARLRAEIR 376 DIDP + + GD R+ L E RL ++ A P +V+ R E Sbjct: 300 DIDPTSIRKNVQVDIPVVGDCRKALAGIREIATTRLAEKDWATAHEPWLTQVSAWRTEQP 359 Query: 377 AAHAPLQALH-QAILDRIAAALPADAFVSTDMTQLAYTGNYAFASRAPRSWLHPTGYGTL 435 + ++ Q +++ I + DA V+T++ Q F R PR+ L G GT+ Sbjct: 360 LTYVKNGSIKPQQVVETIFSITRGDAIVTTEVGQNQMWTAQFFTFRKPRTLLTSGGLGTM 419 Query: 436 GYGLPAGIGAKLGAPQRPGLVLVGDGGFLYTAQELATASEELDSPLVVLLWNNDALGQIR 495 GYG PA IGA+ P + + + GDG QELATA P+ +++ NN LG +R Sbjct: 420 GYGFPAAIGAQFAFPDKLVIDMAGDGSIQMNIQELATAVCN-KLPIKIVILNNGYLGMVR 478 Query: 496 --DDMLGLDIEPVGVLPRNPDFALLGRAYGCAVRQPQDLDELERDLRAGFGQSGVTLIEL 553 ++ + PDF L AYG + + ELE LRA F +I++ Sbjct: 479 QWQELFYQRNYCSTCMEAQPDFVKLAEAYGAEGYRITEAGELESTLRAAFASPHTAVIDV 538 Query: 554 R 554 R Sbjct: 539 R 539 Lambda K H 0.321 0.136 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 761 Number of extensions: 42 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 559 Length of database: 562 Length adjustment: 36 Effective length of query: 523 Effective length of database: 526 Effective search space: 275098 Effective search space used: 275098 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory