Align Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 2.1.2.- (characterized)
to candidate 8502043 DvMF_2756 phosphoribosylglycinamide formyltransferase 2 (RefSeq)
Query= SwissProt::P33221 (392 letters) >FitnessBrowser__Miya:8502043 Length = 393 Score = 492 bits (1266), Expect = e-144 Identities = 248/392 (63%), Positives = 307/392 (78%), Gaps = 1/392 (0%) Query: 1 MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRYADAPAMHVAHRSHVIN 60 M +GT P+ATR++LLGSGELG+EVAIE RLGVEVIAVDRY +APAM VAHRSHV++ Sbjct: 1 MVTIGTPNTPSATRILLLGSGELGREVAIEAMRLGVEVIAVDRYPNAPAMQVAHRSHVVS 60 Query: 61 MLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVVPCARATKLTMNREGIRRL 120 MLDG ALRR++E E+PH IVPEIEAIAT+ L++LE+EG VVP ARA +LTM+REGIRRL Sbjct: 61 MLDGAALRRIIEAERPHCIVPEIEAIATETLLELEKEGFRVVPTARAARLTMDREGIRRL 120 Query: 121 AAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSSSGKGQTFIRSAEQLAQAWKY 180 AAEEL LPTS YRFA++E+ +REAVA +G PC+VKPVMSSSGKGQ+ +R+ + +AW+Y Sbjct: 121 AAEELGLPTSPYRFAETEAEYREAVAAVGLPCVVKPVMSSSGKGQSTVRTEADVMKAWEY 180 Query: 181 AQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFCAPVGHRQEDGDYRESWQPQQMS 240 AQ GGRAG G+VIVEG V FD+EIT LTV G FC P+GH Q+DGDYRESWQP MS Sbjct: 181 AQTGGRAGGGKVIVEGFVDFDYEITQLTVRHAGGTTFCDPIGHLQKDGDYRESWQPHPMS 240 Query: 241 PLALERAQEIARKVVLALGGYGLFGVELFVCGDEVIFSEVSPRPHDTGMVTLISQDLSEF 300 AL ++ +A V ALGG+G+FGVELF+ GD+V FSEVSPRPHDTG+VTLISQ+LSEF Sbjct: 241 QAALAESRRMAEAVTGALGGWGIFGVELFIKGDKVYFSEVSPRPHDTGLVTLISQNLSEF 300 Query: 301 ALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQNVTFDNVQNAVG-ADLQIRLFGKPEID 359 ALH RA LGLPV +RQ GPAAS V+L + S + + ++ A+ D + LFGKPE+ Sbjct: 301 ALHARAILGLPVPALRQNGPAASCVVLAEGDSMSPGYHGIEAALAEPDTGLCLFGKPEVH 360 Query: 360 GSRRLGVALATAESVVDAIERAKHAAGQVKVQ 391 G RR+GVALA S+ +A +A+ AA V+V+ Sbjct: 361 GKRRMGVALALGASIEEARAKARRAAAAVQVE 392 Lambda K H 0.320 0.136 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 468 Number of extensions: 16 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 392 Length of database: 393 Length adjustment: 31 Effective length of query: 361 Effective length of database: 362 Effective search space: 130682 Effective search space used: 130682 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory