Aligments for a candidate for HSERO_RS05250 in Desulfovibrio vulgaris Miyazaki F

GapMind for catabolism of small carbon sources

Aligments for a candidate for HSERO_RS05250 in Desulfovibrio vulgaris Miyazaki F

Align Ribose import ATP-binding protein RbsA; EC 7.5.2.7 (characterized, see rationale)
to candidate 8502321 DvMF_3029 ABC transporter related (RefSeq)

Query= uniprot:D8J111
         (520 letters)



>FitnessBrowser__Miya:8502321
          Length = 537

 Score =  274 bits (701), Expect = 5e-78
 Identities = 165/496 (33%), Positives = 272/496 (54%), Gaps = 18/496 (3%)

Query: 21  PVIALRNVCKRFPGVLALDNCQFELAAGEVHALMGENGAGKSTLMKILSGVYQRDSGDIL 80
           PV+ L  +CK F  V A  +   ++  G + AL+GENGAGKSTLM IL+G  ++D+G I+
Sbjct: 28  PVVRLDGICKSFGKVRANHDITLDIRPGCIKALLGENGAGKSTLMSILAGKLRQDAGTIV 87

Query: 81  LDGKPVEITEPRQAQALGIGIIHQELNLMNHLSAAQNIFIGREPRKAMGLFIDEDELNRQ 140
           +DG P     PR A   GIG+++Q   L++ ++ A+N+ +G+ P     + +    +  +
Sbjct: 88  VDGVPTVFASPRDALRAGIGMVYQHFMLVDSMTVAENVLLGQSP----DMLLRPARMRDE 143

Query: 141 AAAIFARMRLDMDPSTPVGELTVARQQMVEIAKALSFDSRVLIMDEPTAALNNAEIAELF 200
            AA+  R  L +DP+  VG L++  +Q VEI K L  DSRVLI+DEPTA L   E  +LF
Sbjct: 144 VAALAERYGLAVDPAARVGGLSMGERQRVEILKLLYRDSRVLILDEPTAVLTPRETDQLF 203

Query: 201 RIIRDLQAQGVGIVYISHKMDELRQIADRVSVMRDGKYI-----ATVPMQETSMDTIISM 255
             +  +  QG  +V+ISHK+ E+  +AD ++++R G+ +     A VP Q    + ++  
Sbjct: 204 EAMWRMADQGKALVFISHKLQEVLTVADEIAILRRGEVVDEFSEADVPNQTVLANRMVGR 263

Query: 256 MVGRALDGEQRIPPDTSRNDVVLEVRGLNRGRAIRDVSFTLRKGEILGFAGLMGAGRTEV 315
            V   +D ++  P DT     VL V  L+ G  + DVS  +R+GEI+  AG+ G G+ E+
Sbjct: 264 DVVLQVDAKRLTPVDT-----VLSVEHLS-GAGLSDVSLQVRRGEIVAIAGVAGNGQKEL 317

Query: 316 ARAIFGADPLEAGEIIIHGGKAVIKSPADAVAHGIGYLSEDRKHFGLAVGMDVQANIALS 375
             AI G    EAGE+ I G              G+ Y+ EDR+       +D+  N  L+
Sbjct: 318 VEAICGLARPEAGEVRILGRPWREFFAGPPGRRGLAYIPEDRQGLATCRHLDLVDNFLLT 377

Query: 376 SMGRFTRVGFMDQRAIREAAQMYVRQLAIKTPSVEQQARLLSGGNQQKIVIAKWLLRDCD 435
           +  +F +  F+D+     A +  V +  ++   +   AR LSGGN QK+VI +   R  +
Sbjct: 378 TRNQFAKGVFLDRTEATNAVKRVVWEYNVQPGDITAPARALSGGNLQKLVIGREFFRKPE 437

Query: 436 ILFFDEPTRGIDVGAKSEIY-KLLDALAEQGKAIVMISSELPEVLRMSHRVLVMCEGRIT 494
           ++  + PT+G+D+ A  E++ +LL+A +  G  +++++ +L E L ++ R+ VM  GR  
Sbjct: 438 VIVAENPTQGLDISATEEVWGRLLEARSTSG--VLLVTGDLNEALELADRIAVMYRGRFI 495

Query: 495 GELARADATQEKIMQL 510
               + D  + + + L
Sbjct: 496 DVFDKDDTAKVQAIGL 511



 Score = 65.1 bits (157), Expect = 6e-15
 Identities = 46/224 (20%), Positives = 101/224 (45%), Gaps = 5/224 (2%)

Query: 38  LDNCQFELAAGEVHALMGENGAGKSTLMKILSGVYQRDSGDILLDGKPVEITEPRQAQAL 97
           L +   ++  GE+ A+ G  G G+  L++ + G+ + ++G++ + G+P            
Sbjct: 291 LSDVSLQVRRGEIVAIAGVAGNGQKELVEAICGLARPEAGEVRILGRPWREFFAGPPGRR 350

Query: 98  GIGII---HQELNLMNHLSAAQNIFIGREPRKAMGLFIDEDELNRQAAAIFARMRLDM-D 153
           G+  I    Q L    HL    N  +    + A G+F+D  E       +     +   D
Sbjct: 351 GLAYIPEDRQGLATCRHLDLVDNFLLTTRNQFAKGVFLDRTEATNAVKRVVWEYNVQPGD 410

Query: 154 PSTPVGELTVARQQMVEIAKALSFDSRVLIMDEPTAALNNAEIAELFRIIRDLQAQGVGI 213
            + P   L+    Q + I +       V++ + PT  L+ +   E++  + + ++   G+
Sbjct: 411 ITAPARALSGGNLQKLVIGREFFRKPEVIVAENPTQGLDISATEEVWGRLLEARSTS-GV 469

Query: 214 VYISHKMDELRQIADRVSVMRDGKYIATVPMQETSMDTIISMMV 257
           + ++  ++E  ++ADR++VM  G++I      +T+    I +M+
Sbjct: 470 LLVTGDLNEALELADRIAVMYRGRFIDVFDKDDTAKVQAIGLMM 513


Lambda     K      H
   0.320    0.135    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 627
Number of extensions: 32
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 520
Length of database: 537
Length adjustment: 35
Effective length of query: 485
Effective length of database: 502
Effective search space:   243470
Effective search space used:   243470
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources