Alignments for a candidate for fumD in Desulfovibrio vulgaris Miyazaki F

GapMind for catabolism of small carbon sources

Alignments for a candidate for fumD in Desulfovibrio vulgaris Miyazaki F

Align fumarate hydratase (EC 4.2.1.2); (S)-2-methylmalate dehydratase (EC 4.2.1.34) (characterized)
to candidate 8501745 DvMF_2461 fumarate hydratase (RefSeq)

Query= BRENDA::Q141Z6
         (520 letters)



>FitnessBrowser__Miya:8501745
          Length = 279

 Score =  186 bits (473), Expect = 7e-52
 Identities = 113/283 (39%), Positives = 158/283 (55%), Gaps = 16/283 (5%)

Query: 22  LIQSIADSLQYISYYHPLDYIQALGRAYELEQSPAAKDAIAQILTNSRMCAEGKRPICQD 81
           +++ +A      + Y P D   A  RA   E+S  AK+   Q+L N+ + A    P+CQD
Sbjct: 9   VVEEVARLCVECNRYLPKDVRWAFCRAQAEEESDVAKEVFRQLLENADLAATTGLPLCQD 68

Query: 82  TGIVTVFVKVGMDVRWDGATMGVTDMINEGVRRGYLNPDNVLRASIVSPPEGGRKNTKDN 141
           TG+   FV++G D R DG T+   + I EGVR GY   +  LR S   P    RKNT DN
Sbjct: 69  TGLAVFFVELGEDCRVDGMTL--REAITEGVRIGYA--EGYLRKSACDPMT--RKNTGDN 122

Query: 142 TPAVIHYEIVPGNTVDVQVAAKGGGSENKSKFAMLNPS---DSIVDWILKTVPTMGAGWC 198
           TPAVIH ++VPG+ + +   AKGGGSEN S+  ML P+     I D++++ V   G   C
Sbjct: 123 TPAVIHMDLVPGDRLRIAFMAKGGGSENMSRVTMLAPAQGWQGIKDFVVQRVREAGPNPC 182

Query: 199 PPGMLGIGIGGTAEKAMVMAKESLMDPI-DIQDVIARGPKDWIEELRVELHEKVNALGIG 257
           PP ++G+G+GGT + A ++AK++L+ P+ DI       P   +     EL   +N LGIG
Sbjct: 183 PPTIIGVGVGGTFDYAPILAKKALLRPLTDIH------PDPDMAAKEAELLAAINELGIG 236

Query: 258 AQGLGGLATVLDVKIMAAPTHAASKPVAIIPNCAATRHAHFTL 300
             GLGG  T L VK+  AP H AS P+A+   C + RH    L
Sbjct: 237 PMGLGGKTTCLGVKMAFAPCHLASLPLAVNVQCHSVRHGEIEL 279


Lambda     K      H
   0.316    0.133    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 344
Number of extensions: 17
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 520
Length of database: 279
Length adjustment: 30
Effective length of query: 490
Effective length of database: 249
Effective search space:   122010
Effective search space used:   122010
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)

Align fumarate hydratase (EC 4.2.1.2); (S)-2-methylmalate dehydratase (EC 4.2.1.34) (characterized)
to candidate 8501746 DvMF_2462 hydro-lyase, Fe-S type, tartrate/fumarate subfamily, beta subunit (RefSeq)

Query= BRENDA::Q141Z6
         (520 letters)



>FitnessBrowser__Miya:8501746
          Length = 175

 Score =  131 bits (329), Expect = 2e-35
 Identities = 73/166 (43%), Positives = 98/166 (59%), Gaps = 1/166 (0%)

Query: 336 LTPEEVAAWTPGQTLLLSGKMLTGRDAAHKRIADMLAKGEKLPVDFTNRVIYYVGPVDPV 395
           LT E VA    G  + LSG + T RDAAH+R+A+ L +GE  P D    VIYYVGP    
Sbjct: 10  LTDEAVAQLRSGDVVFLSGTIYTARDAAHRRLAESLDRGEDPPFDLRGAVIYYVGPSPAP 69

Query: 396 RDEAVGPAGPTTATRMDKFTEMMLAQTGLISMIGKAERGPVAIEAIRKHKAAYLMAVGGA 455
               +G AGPTT+ RMD +   + A  GL + IGK  R     +A+++H A YL A GGA
Sbjct: 70  PGRPIGSAGPTTSYRMDSYAPRLHA-LGLKATIGKGRRNTEVRDALKQHTAVYLGATGGA 128

Query: 456 AYLVSKAIRSAKVLAFEDLGMEAIYEFDVQDMPVTVAVDSNGTSVH 501
             L+S+ I +A V+A++DLG EAI E  V+D P+ V  D  G  ++
Sbjct: 129 GALLSQCITAATVIAYDDLGPEAIRELTVKDFPLLVINDCVGGELY 174


Lambda     K      H
   0.316    0.133    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 256
Number of extensions: 8
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 520
Length of database: 175
Length adjustment: 26
Effective length of query: 494
Effective length of database: 149
Effective search space:    73606
Effective search space used:    73606
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources