Align Aconitate hydratase A; ACN; Aconitase; (2R,3S)-2-methylisocitrate dehydratase; (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; Iron-responsive protein-like; IRP-like; Probable 2-methyl-cis-aconitate hydratase; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.99 (characterized)
to candidate 8502313 DvMF_3021 aconitate hydratase (RefSeq)
Query= SwissProt::Q5SMF6 (902 letters) >FitnessBrowser__Miya:8502313 Length = 641 Score = 219 bits (559), Expect = 3e-61 Identities = 200/664 (30%), Positives = 269/664 (40%), Gaps = 171/664 (25%) Query: 172 PPGTGIVHQVNIEYLTKVVMTGKRDGLTLAFPDS-LVGTDSHTTMVNGLGVLGWGVGGIE 230 PPGTGI HQ+++E A P + LVG+DSHT G+G L G GG+ Sbjct: 94 PPGTGICHQLHLE--------------NFALPGATLVGSDSHTPTAGGIGSLAMGAGGLS 139 Query: 231 AEAVMLGQPYYMLAPRVVGFKLYGELPEGATATDLVLTVTEMLRKHGVVGKFVEFYGPGV 290 M G+PY++ PRVV +L G L A+A D++L + +L G VGK EF GPGV Sbjct: 140 VALAMAGEPYFISMPRVVRVRLEGRLTGWASAKDVILHLLGLLTVKGGVGKVFEFAGPGV 199 Query: 291 AKLSTPDRATIANMAPEYGATMGFFPVDEETLNYLRQTGRPEELVELVEAYTKAVGLFRT 350 A LS P+RA I NM E GAT FP DE T +L R G ++ Sbjct: 200 ATLSVPERAVITNMGAELGATASLFPSDERTRAFLASMDRE--------------GDWKP 245 Query: 351 PEAEEKVQYSEYLELDLSAVEPSLAGPKRPQDRVPLKEVKKSFLAHLTKPVKERGFGLSE 410 A+ Y + + +DLSA+ P +A P P VP+ E+ GLS Sbjct: 246 LAADADATYDDEIVIDLSALVPLVAQPHMPDRVVPVAELA----------------GLSV 289 Query: 411 DQLQRKVLVKRRDEEFELTHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVEAGLDRKPW 470 DQ+ AI SCTN+S + + A +L + V G D Sbjct: 290 DQV-----------------------AIGSCTNSSYADMRMVAEVLRGRMVHVGTD---- 322 Query: 471 VKTSLAPGSKVVTDYLEMSGLMPFLEALGFHLVGYGCTTCIGNSGPLPEDIAKAVEEGNL 530 T ++PGSK V L GL+ L G ++ C CIG G V G Sbjct: 323 --TMVSPGSKQVLKMLAAEGLVEPLLDAGVRILECSCGPCIGMGG-------SPVSGG-- 371 Query: 531 VVAAVLSGNRNFEGRINPHVKANYLASPMLVVAYALAGRMDIDFTTEPLGFDPNGKPIYL 590 +V + NRNFEGR YL SP+ AL G+ T+P + G P Sbjct: 372 --VSVRTFNRNFEGRSGTKDAKVYLVSPLTAAMAALHGQF-----TDPATW---GTPPAQ 421 Query: 591 KDIWPSMEEIREAIRKTLDPELFKKEYSKVFEGDERWQALPAPTGELYQWDPESTYIQNP 650 ++ IR A P G + ++ P Sbjct: 422 PELPAKAPSIRHLF------------------------AFPPRDGSGVE------VLRGP 451 Query: 651 PFFEDLGERKVEDIRGARVLLVLGDSVTTDHISPAGAIPVKSPAGQYLISKGVKPEDFNS 710 ++D A V++ LGD +TTDHI PAGA Sbjct: 452 NIVALEQFTPMDDTVAAPVVIKLGDDITTDHIMPAGA----------------------E 489 Query: 711 YGSRRGNHEVMMRGTFANIRIKNLMLDGIEGGYAKKLPEGDVDFVYNVAMRYKAEGTPLL 770 S R N + + F + D DFV R +A G + Sbjct: 490 ITSLRSNVPAIAQHVFGRV---------------------DADFV----ARARAAGIG-V 523 Query: 771 VIAGKEYGTGSSRDWAAKGTYLLGIRAVLAESFERIHRSNLVGMGVLPLEFLPGENRETL 830 ++AG YG GSSR+ AA LGIRAV+ S RIHR+NLV G+LPL + + L Sbjct: 524 IVAGDNYGQGSSREHAALAPRHLGIRAVIVRSLARIHRANLVNFGILPLILCDRTDYDKL 583 Query: 831 GLTG 834 + G Sbjct: 584 AVGG 587 Lambda K H 0.317 0.137 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1274 Number of extensions: 75 Number of successful extensions: 8 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 3 Number of HSP's successfully gapped: 3 Length of query: 902 Length of database: 641 Length adjustment: 40 Effective length of query: 862 Effective length of database: 601 Effective search space: 518062 Effective search space used: 518062 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 55 (25.8 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory